Bacteriocin: curvacin-A
Accession: BAC065
Classification
Class: class IIA/YGNGV
Genetics
Gene: curA
Producer and target organisms
Producer Organism: Lactobacillus curvatus [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsclosely related Lactobacilli - Listeria monocytogenes - Listeria innocua - Listeria ivanovii - Enterococcus faecalis - Carnobacterium sp - Brochothrix thermosphacta
Uniprot and PDB links
UniProt Entry: P0A311
PDB Entry2A2B resolved by NMR.
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=LTH1174
MEDLINE=94058500;PubMed=7694558;DOI=10.1007/BF00292077
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174.", Arch. Microbiol. 160:279-283(1993).
MEDLINE=94058500;PubMed=7694558;DOI=10.1007/BF00292077
Tichaczek P.S., Vogel R.F., Hammes W.P.
"Cloning and sequencing of curA encoding curvacin A, the bacteriocin produced by Lactobacillus curvatus LTH1174.", Arch. Microbiol. 160:279-283(1993).
[Ref. 2]
PRELIMINARY PARTIAL PROTEIN SEQUENCE OF 19-59, STRAIN=LTH1174
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).
Tichaczek P.S., Nissen-Meyer J., Nes I.F., Vogel R.F., Hammes W.P.
"Characterization of the bacteriocins curvacin A from Lactobacillus curvatus LTH1174 and sakacin P from L. sake LTH673.", Syst. Appl. Microbiol. 15:460-465(1992).
Mutational Analysis / Structure-function relationships
PubMed=18791005;DOI=10.1128/AEM.01068-08
Haugen HS, Kristiansen PE, Fimland G, Nissen-Meyer J
"Mutational Analysis of the Class IIa Bacteriocin Curvacin A and Its Orientation in Target Cell Membranes.", Appl. Environ. Microbiol. 74(21):6766-73 (2008).
PubMed=18791005;DOI=10.1128/AEM.01068-08
Haugen HS, Kristiansen PE, Fimland G, Nissen-Meyer J
"Mutational Analysis of the Class IIa Bacteriocin Curvacin A and Its Orientation in Target Cell Membranes.", Appl. Environ. Microbiol. 74(21):6766-73 (2008).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene229 | curA | pre-curvacinA | 252..431 |
| BACGene230 | curA | ORFB | 586..741 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ARSYGNGVYC NNKKCWVNRG EATQSIIGGM ISGWASGLAG M |
Wheel representation
3D Structure
View PDB entry: 2A2B
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔41 | 41 | Bacteriocin curvacin-A. Feature identifier = PRO_0000002748. |
| DISULFID | 10↔15 | 6 | |
| CONFLICT | 2 | R -> C (in Ref. 2; AA sequence). | |
| CONFLICT | 15 | C -> S (in Ref. 2; AA sequence). | |
| STRAND | 4↔6 | 3 | |
| STRAND | 11↔14 | 4 | |
| HELIX | 19↔24 | 6 | |
| HELIX | 29↔39 | 11 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 4 | 9.76 % |
| C | 2 | 4.88 % |
| D | 0 | 0.00 % |
| E | 1 | 2.44 % |
| F | 0 | 0.00 % |
| G | 8 | 19.51 % |
| H | 0 | 0.00 % |
| I | 3 | 7.32 % |
| K | 2 | 4.88 % |
| L | 1 | 2.44 % |
| M | 2 | 4.88 % |
| N | 4 | 9.76 % |
| P | 0 | 0.00 % |
| Q | 1 | 2.44 % |
| R | 2 | 4.88 % |
| S | 4 | 9.76 % |
| T | 1 | 2.44 % |
| V | 2 | 4.88 % |
| W | 2 | 4.88 % |
| Y | 2 | 4.88 % |
Hydrophobicity
Composition
| Formula | C184
H288
N56
O56
S4 |
| Absent amino acids | DFHP |
| Common amino acids | G |
| Mass (Da) | 4327.58 |
| Net charge | +3 |
| Isoelectric point | 9.37 |
| Basic residues | 4 |
| Acidic residues | 1 |
| Hydrophobic residues | 12 |
| Polar residues | 21 |
| Aliphatic residues | 6 |
| Tiny residues | 16 |
| Boman Index | -41.86 |
| Hydropathy Index | -0.19 |
| Aliphatic Index | 61.95 |
| Instability Index | 27.63 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 352.63 |