Bacteriocin: lactococcin-G β
Accession: BAC053
Classification
Class: Unclassified
Genetics
Gene: LcnG-beta
Producer and target organisms
Producer Organism: Lactococcus lactis subsp (Streptococcus lactis), lactis [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismsUnavailable data
Description
Mode of action:
Kills Lactococci. beta peptides in a molar ratio of 7:1 or 8:1 respectively.
Kills Lactococci. beta peptides in a molar ratio of 7:1 or 8:1 respectively.
Uniprot and PDB links
UniProt Entry: P36962
PDB Entry2JPK resolved by NMR (lcnG-b in the presence of DPC micelles) 2JPM resolved by NMR (lcnG-b in TFE)
PROTEIN SEQUENCE, STRAIN=LMG 2081
MEDLINE=92380946;PubMed=1512201;
Nissen-Meyer J., Holo H., Havarstein L.S., Sletten K., Nes I.F.
"A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides.", J. Bacteriol. 174:5686-5692(1992).
MEDLINE=92380946;PubMed=1512201;
Nissen-Meyer J., Holo H., Havarstein L.S., Sletten K., Nes I.F.
"A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides.", J. Bacteriol. 174:5686-5692(1992).
PROTEIN STRUCTURE
PubMed=18187052;DOI=10.1016/j.bbapap.2007.12.002
Per Rogne, Gunnar Fimland, Jon Nissen-Meyer and Per Eugen Kristiansen.
"Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin lactococcin G",
PubMed=18187052;DOI=10.1016/j.bbapap.2007.12.002
Per Rogne, Gunnar Fimland, Jon Nissen-Meyer and Per Eugen Kristiansen.
"Three-dimensional structure of the two peptides that constitute the two-peptide bacteriocin lactococcin G",
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | KKWGWLAWVD PAYEFIKGFG KGAIKEGNKD KWKNI |
Wheel representation
3D Structure
View PDB entry: 2JPK
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔35 | 35 | Bacteriocin lactococcin-G subunit beta. Feature identifier = PRO_0000044641. |
| HELIX | 5↔19 | 15 | |
| TURN | 20↔22 | 3 | |
| TURN | 24↔26 | 3 | |
| HELIX | 32↔34 | 3 |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 8.57 % |
| C | 0 | 0.00 % |
| D | 2 | 5.71 % |
| E | 2 | 5.71 % |
| F | 2 | 5.71 % |
| G | 5 | 14.29 % |
| H | 0 | 0.00 % |
| I | 3 | 8.57 % |
| K | 8 | 22.86 % |
| L | 1 | 2.86 % |
| M | 0 | 0.00 % |
| N | 2 | 5.71 % |
| P | 1 | 2.86 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 0 | 0.00 % |
| T | 0 | 0.00 % |
| V | 1 | 2.86 % |
| W | 4 | 11.43 % |
| Y | 1 | 2.86 % |
Hydrophobicity
Composition
| Formula | C198
H291
N49
O47
|
| Absent amino acids | CHMQRST |
| Common amino acids | K |
| Mass (Da) | 4128.38 |
| Net charge | +4 |
| Isoelectric point | 10.42 |
| Basic residues | 8 |
| Acidic residues | 4 |
| Hydrophobic residues | 14 |
| Polar residues | 8 |
| Aliphatic residues | 5 |
| Tiny residues | 8 |
| Boman Index | -39.75 |
| Hydropathy Index | -0.81 |
| Aliphatic Index | 61.43 |
| Instability Index | 10.35 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 23490 M-1 cm-1 |
| Absorbance 280nm | 690.88 |