Bacteriocin: Enterocin Q
Accession: BAC052
Classification
Class: Class IIc
Genetics
Gene: entQ
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsLactobacillus sakei - Enterococcus faecium
Description
Enterococcus faecium L50 produces, in addition to EntL50, the sec-dependent pediocin-like enterocin P (EntP), and an unmodified non-pediocin-like bacteriocin, termed enterocin Q (EntQ), synthesized without an N-terminal leader sequence or signal peptide. Note=Ref1.
Uniprot and PDB links
UniProt Entry: Q7WYZ9
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=L50
MEDLINE=20528333;PubMed=11073927;DOI=10.1128/JB.182.23.6806-6814.2000
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q.", J. Bacteriol. 182:6806-6814(2000).
MEDLINE=20528333;PubMed=11073927;DOI=10.1128/JB.182.23.6806-6814.2000
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"Biochemical and genetic evidence that Enterococcus faecium L50 produces enterocins L50A and L50B, the sec-dependent enterocin P, and a novel bacteriocin secreted without an N-terminal extension termed enterocin Q.", J. Bacteriol. 182:6806-6814(2000).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=L50
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"", Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
Cintas L.M., Casaus P., Herranz C., Havarstein L.S., Holo H., Hernandez P.E., Nes I.F.
"", Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene186 | entqA | EntQ leader-less bacteriocin; enterocin Q | complement(155..259) |
| BACGene187 | entqB | EntQB EntQ ABC-transporter | 340..2058 |
| BACGene188 | entqC | EntQC EntQ immunity protein | 2069..2272 |
| BACGene189 | entqC | putative mobilization protein ORF4 | 2686..3012 |
| BACGene190 | entqC | putative mobilization protein ORF5; contains relaxase/mobilization nuclease domain | 2994..3908 |
| BACGene191 | entqC | hypothetical protein ORF6 | 3700..4146 |
| BACGene192 | entqC | hypothetical protein ORF7 | 4148..4837 |
| BACGene193 | entqC | putative plasmid replication protein ORF8 | 5690..6427 |
| BACGene194 | entqC | putative plasmid replication protein ORF9 | 6455..6991 |
| BACGene195 | entqC | hypothetical protein ORF10 | 7007..7177 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | MNFLKNGIAK WMTGAELQAY KKKYGCLPWE KISC |
Wheel representation
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 3 | 8.82 % |
| C | 2 | 5.88 % |
| D | 0 | 0.00 % |
| E | 2 | 5.88 % |
| F | 1 | 2.94 % |
| G | 3 | 8.82 % |
| H | 0 | 0.00 % |
| I | 2 | 5.88 % |
| K | 6 | 17.65 % |
| L | 3 | 8.82 % |
| M | 2 | 5.88 % |
| N | 2 | 5.88 % |
| P | 1 | 2.94 % |
| Q | 1 | 2.94 % |
| R | 0 | 0.00 % |
| S | 1 | 2.94 % |
| T | 1 | 2.94 % |
| V | 0 | 0.00 % |
| W | 2 | 5.88 % |
| Y | 2 | 5.88 % |
Hydrophobicity
Composition
| Formula | C181
H281
N45
O46
S4 |
| Absent amino acids | DHRV |
| Common amino acids | K |
| Mass (Da) | 3970.31 |
| Net charge | +4 |
| Isoelectric point | 9.78 |
| Basic residues | 6 |
| Acidic residues | 2 |
| Hydrophobic residues | 11 |
| Polar residues | 11 |
| Aliphatic residues | 5 |
| Tiny residues | 7 |
| Boman Index | -24.24 |
| Hydropathy Index | -0.36 |
| Aliphatic Index | 66.18 |
| Instability Index | 21.66 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 14105 M-1 cm-1 |
| Absorbance 280nm | 427.42 |