Bacteriocin: leucocin-B
Accession: BAC042
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Leuconostoc mesenteroides [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLeuconostoc
Target organismsUnknown error type: [8] Uninitialized string offset: -1
wide spectrum of lactic acid bacteria.
Description
Leuconostoc mesenteroides TA33a produces three bacteriocins leucocins A-, B- and C. Leucocin B consists of 31 amino acid residues, with a molecular mass of 3466 Da. Leucocin B-TA33a does not belong to the pediocin family of bacteriocins, but shares 62% homology with mesenterocin 52B.
Circular dichroism spectra of the leucocins in aqueous solution and micellar SDS indicated that they undergo a structural transition when in a membrane-mimicking environment. Theoretical predictions from circular dichroism data suggest that leucocins B adopt a beta-structure (48%) in membrane-mimicking environments.
Circular dichroism spectra of the leucocins in aqueous solution and micellar SDS indicated that they undergo a structural transition when in a membrane-mimicking environment. Theoretical predictions from circular dichroism data suggest that leucocins B adopt a beta-structure (48%) in membrane-mimicking environments.
Uniprot and PDB links
UniProt Entry: P81052
PDB EntryUnknown
PROTEIN SEQUENCE, STRAIN=TA33a
MEDLINE=98274743;PubMed=9611809;
Papathanasopoulos M.A., Dykes G.A., Revol-Junelles A.-M., Delfour A., von Holy A., Hastings J.W.
"Sequence and structural relationships of leucocins A-, B- and C-TA33a from Leuconostoc mesenteroides TA33a.", Microbiology 144:1343-1348(1998).
MEDLINE=98274743;PubMed=9611809;
Papathanasopoulos M.A., Dykes G.A., Revol-Junelles A.-M., Delfour A., von Holy A., Hastings J.W.
"Sequence and structural relationships of leucocins A-, B- and C-TA33a from Leuconostoc mesenteroides TA33a.", Microbiology 144:1343-1348(1998).
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | KGKGFWSWAS KATSWLTGPQ QPGSPLLKKH R |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔31 | 31 | Bacteriocin leucocin-B. Feature identifier = PRO_0000044639. |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 2 | 6.45 % |
| C | 0 | 0.00 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 1 | 3.23 % |
| G | 4 | 12.90 % |
| H | 1 | 3.23 % |
| I | 0 | 0.00 % |
| K | 5 | 16.13 % |
| L | 3 | 9.68 % |
| M | 0 | 0.00 % |
| N | 0 | 0.00 % |
| P | 3 | 9.68 % |
| Q | 2 | 6.45 % |
| R | 1 | 3.23 % |
| S | 4 | 12.90 % |
| T | 2 | 6.45 % |
| V | 0 | 0.00 % |
| W | 3 | 9.68 % |
| Y | 0 | 0.00 % |
Hydrophobicity
Composition
| Formula | C161
H246
N46
O40
|
| Absent amino acids | CDEIMNVY |
| Common amino acids | K |
| Mass (Da) | 3484.5 |
| Net charge | +7 |
| Isoelectric point | 12.05 |
| Basic residues | 7 |
| Acidic residues | 0 |
| Hydrophobic residues | 9 |
| Polar residues | 10 |
| Aliphatic residues | 3 |
| Tiny residues | 10 |
| Boman Index | -45.04 |
| Hydropathy Index | -0.97 |
| Aliphatic Index | 44.19 |
| Instability Index | 27.93 (stable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 16500 M-1 cm-1 |
| Absorbance 280nm | 550 |