Bacteriocin: mutacin 1140 (Mutacin III)
Accession: BAC020
Classification
Class: Lantibiotic
Genetics
Gene: lanA; Synonyms=mutA
Producer and target organisms
Producer Organism: Streptococcus mutans [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor.
Uniprot and PDB links
UniProt Entry: O68586
PDB EntryUnknown
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=JH1005
MEDLINE=98261521;PubMed=9596742;
Hillman J.D., Novak J., Sagura E., Gutierrez J.A., Brooks T.A., Crowley P.J., Hess M., Azizi A., Leung K.-P., Cvitkovitch D., Bleiweis A.S.
"Genetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans.", Infect. Immun. 66:2743-2749(1998).
MEDLINE=98261521;PubMed=9596742;
Hillman J.D., Novak J., Sagura E., Gutierrez J.A., Brooks T.A., Crowley P.J., Hess M., Azizi A., Leung K.-P., Cvitkovitch D., Bleiweis A.S.
"Genetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans.", Infect. Immun. 66:2743-2749(1998).
NUCLEOTIDE SEQUENCE [GENOMIC DNA], STRAIN=UA787
MEDLINE=99402724;PubMed=10473390;
Qi F., Chen P., Caufield P.W.
"Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes.", Appl. Environ. Microbiol. 65:3880-3887(1999).
MEDLINE=99402724;PubMed=10473390;
Qi F., Chen P., Caufield P.W.
"Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutacin III biosynthesis genes.", Appl. Environ. Microbiol. 65:3880-3887(1999).
PROTEIN SEQUENCE OF 42-63, MASS SPECTROMETRY, AND STRUCTURE BY NMR.
MEDLINE=20534754;PubMed=11082191;
Smith L., Novak J., Rocca J., McClung S., Hillman J.D., Edison A.S.
"Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics.", Eur. J. Biochem. 267:6810-6816(2000).
MEDLINE=20534754;PubMed=11082191;
Smith L., Novak J., Rocca J., McClung S., Hillman J.D., Edison A.S.
"Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics.", Eur. J. Biochem. 267:6810-6816(2000).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene44 | mutR | MutR transcription regulator; similar to Streptococcus gordonii Rgg and Streptococcusmutans mutacin II operon encoded MutR | 205..1059 |
| BACGene45 | mutA | MutA precursor prepropeptide of mutacin III; identical to mutacin 1140; similar to mutacinNY266, epidermin and gallidermin | 1646..1837 |
| BACGene46 | mutA' | MutA' similar to MutA; not required for mutacin III production. | 1904..2098 |
| BACGene47 | mutB | MutB modifying enzyme for promutacin III; similar to SpaB, NisB and EpiB | 2253..5225 |
| BACGene48 | mutC | MutC modifying enzyme for promutacin III; similar to EpiC, SpaC, NisC, EciC and PepC | 5212..6486 |
| BACGene49 | mutD | MutD may function in C-terminal oxadative decarboxylation of promutacin III modifying enzyme for promutacin III; similar to EpiD and other flavoproteins | 6509..7075 |
| BACGene50 | mutP | MutP processing of premutacin III serine protease; similar to NisP, EpiP and serine proteases | 7080..8423 |
| BACGene51 | mutT | MutT transportation of premutacin III ABC transporter; similar to LmrA, PepT and ABC transporters | 8424..10049 |
Protein sequence
| ........10 ........20 ........30 | | | FKSWSLCTPG CARTGSFNSY CC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔22 | 22 | Lantibiotic mutacin-1140. Feature identifier = PRO_0000017131. |
| Modified residue | 5 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 14 | 2,3-didehydrobutyrine. | |
| Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 16↔21 | 6 | Lanthionine (Ser-Cys). |
| Cross-link | 19↔22 | 4 | S-(2-aminovinyl)-D-cysteine (Ser-Cys). |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 1 | 4.55 % |
| C | 4 | 18.18 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 2 | 9.09 % |
| G | 2 | 9.09 % |
| H | 0 | 0.00 % |
| I | 0 | 0.00 % |
| K | 1 | 4.55 % |
| L | 1 | 4.55 % |
| M | 0 | 0.00 % |
| N | 1 | 4.55 % |
| P | 1 | 4.55 % |
| Q | 0 | 0.00 % |
| R | 1 | 4.55 % |
| S | 4 | 18.18 % |
| T | 2 | 9.09 % |
| V | 0 | 0.00 % |
| W | 1 | 4.55 % |
| Y | 1 | 4.55 % |
Hydrophobicity
Composition
| Formula | C104
H152
N28
O31
S4 |
| Absent amino acids | DEHIMQV |
| Common amino acids | CS |
| Mass (Da) | 2437.1 |
| Net charge | +2 |
| Isoelectric point | 8.34 |
| Basic residues | 2 |
| Acidic residues | 0 |
| Hydrophobic residues | 5 |
| Polar residues | 14 |
| Aliphatic residues | 1 |
| Tiny residues | 7 |
| Boman Index | -23.97 |
| Hydropathy Index | 0 |
| Aliphatic Index | 22.27 |
| Instability Index | 13.67 (stable) |
| Half Life |
Mammalian : 1.1 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 7240 M-1 cm-1 |
| Absorbance 280nm | 344.76 |