Bacteriocin: mutacin B-Ny266
Accession: BAC019
Classification
Class: Lantibiotic
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Streptococcus mutans [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor. RANGE=1-22; NOTE=Ref.1.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The C-terminal lanthionine undergoes decarboxylation. This is followed by membrane translocation and cleavage of the modified precursor. RANGE=1-22; NOTE=Ref.1.
Uniprot and PDB links
UniProt Entry: P80666
PDB EntryUnknown
PROTEIN SEQUENCE, AND MASS SPECTROMETRY, STRAIN=Ny266
MEDLINE=97379322;PubMed=9237644;DOI=10.1016/S0014-5793(97)00425-0
Mota-Meira M., Lacroix C., Lapointe G., Lavoie M.C.
"Purification and structure of mutacin B-Ny266: a new lantibiotic produced by Streptococcus mutans.", FEBS Lett. 410:275-279(1997).
MEDLINE=97379322;PubMed=9237644;DOI=10.1016/S0014-5793(97)00425-0
Mota-Meira M., Lacroix C., Lapointe G., Lavoie M.C.
"Purification and structure of mutacin B-Ny266: a new lantibiotic produced by Streptococcus mutans.", FEBS Lett. 410:275-279(1997).
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Protein sequence
| ........10 ........20 ........30 | | | FKSWSFCTPG CAKTGSFNSY CC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔22 | 22 | Lantibiotic mutacin B-Ny266. Feature identifier = PRO_0000043969. |
| Modified residue | 5 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 14 | 2,3-didehydrobutyrine. | |
| Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 16↔21 | 6 | Lanthionine (Ser-Cys). |
| Cross-link | 19↔22 | 4 | S-(2-aminovinyl)-D-cysteine (Ser-Cys). |
Composition
| Amino acid | Count | Percent |
|---|---|---|
| A | 1 | 4.55 % |
| C | 4 | 18.18 % |
| D | 0 | 0.00 % |
| E | 0 | 0.00 % |
| F | 3 | 13.64 % |
| G | 2 | 9.09 % |
| H | 0 | 0.00 % |
| I | 0 | 0.00 % |
| K | 2 | 9.09 % |
| L | 0 | 0.00 % |
| M | 0 | 0.00 % |
| N | 1 | 4.55 % |
| P | 1 | 4.55 % |
| Q | 0 | 0.00 % |
| R | 0 | 0.00 % |
| S | 4 | 18.18 % |
| T | 2 | 9.09 % |
| V | 0 | 0.00 % |
| W | 1 | 4.55 % |
| Y | 1 | 4.55 % |
Hydrophobicity
Composition
| Formula | C107
H150
N26
O31
S4 |
| Absent amino acids | DEHILMQRV |
| Common amino acids | CS |
| Mass (Da) | 2443.1 |
| Net charge | +2 |
| Isoelectric point | 8.33 |
| Basic residues | 2 |
| Acidic residues | 0 |
| Hydrophobic residues | 5 |
| Polar residues | 14 |
| Aliphatic residues | 0 |
| Tiny residues | 7 |
| Boman Index | -16.54 |
| Hydropathy Index | -0.01 |
| Aliphatic Index | 4.55 |
| Instability Index | 13.67 (stable) |
| Half Life |
Mammalian : 1.1 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 7240 M-1 cm-1 |
| Absorbance 280nm | 344.76 |