Bacteriocin: enterocin NKR-5-3B (Ent53B)
Accession: BAC229
Classification
Class: Circular
Genetics
Gene: EntkB
Producer and target organisms
Producer Organism: Enterococcus faecalis NKR-5-3 [Gram-positive]
Taxonomy: cellular organisms
BacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Target organismsBacillus coagulans JCM 2257 (MIC=0.29μM) - Bacillus subtilis JCM 1465 (MIC=0.57μM) - Kocuria rhizophila NBRC 12708 (MIC=1.1μM) - Listeria innocua ATCC 33090 (MIC=2.3μM) - Pediococcus pentosaceus JCM 5885 (MIC=0.57μM) - Enterococcus faecalis JCM 5803 (MIC=1.1μM) - Lactococcus lactis ssp. Lactis ATCC 19435 (MIC=0.29μM) - Lactobacillus plantarum ATCC 14917 (MIC=1.1μM) - Lactobacillus sakei ssp. Sakei JCM 1157 (MIC=0.57μM)
Description
NULL
Uniprot and PDB links
UniProt Entry: A0A0M3KKS4
PDB Entry2MP8 resolved by NMR
STRUCTURE
PubMed=26174911;DOI=10.1021/acs.biochem.5b00196
Himeno K., Rosengren K.J., Inoue T., Perez R.H., Colgrave M.L., Lee H.S., Chan L.Y., Henriques S.T., Fujita K., Ishibashi N., Zendo T., Wilaipun P., Nakayama J., Leelawatcharamas V., Jikuya H., Craik D.J., Sonomoto K.
"Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium.", Biochemistry 2015, 54 (31):4863-76.
PubMed=26174911;DOI=10.1021/acs.biochem.5b00196
Himeno K., Rosengren K.J., Inoue T., Perez R.H., Colgrave M.L., Lee H.S., Chan L.Y., Henriques S.T., Fujita K., Ishibashi N., Zendo T., Wilaipun P., Nakayama J., Leelawatcharamas V., Jikuya H., Craik D.J., Sonomoto K.
"Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium.", Biochemistry 2015, 54 (31):4863-76.
IDENTIFICATION
PubMed=22738965;DOI=10.1271/bbb.110972
Ishibashi N., Himeno K., Fujita K., Masuda Y., Perez R.H., Zendo T., Wilaipun P., Leelawatcharamas V., Nakayama J., Sonomoto K.
"Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.", Biosci Biotechnol Biochem 2012, 76 (5):947-53.
PubMed=22738965;DOI=10.1271/bbb.110972
Ishibashi N., Himeno K., Fujita K., Masuda Y., Perez R.H., Zendo T., Wilaipun P., Leelawatcharamas V., Nakayama J., Sonomoto K.
"Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.", Biosci Biotechnol Biochem 2012, 76 (5):947-53.
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Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 | | | | | | | LTANLGISSY AAKKVIDIIN TGSAVATIIA LVTAVVGGGL ITAGIVATAK SLIKKYGAKY AAAW |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔64 | 64 | Enterocin NKR-5-3B.
Feature identifier = |
| HELIX | 9↔21 | 13 | |
| HELIX | 25↔36 | 12 | |
| HELIX | 43↔55 | 13 | |
| HELIX | 57↔64 | 8 | |
| Cross-link | 1↔64 | 64 |
Composition
Hydrophobicity
Composition
| Formula | C
H
N
O1
|
| Absent amino acids | CEFHMPQR |
| Common amino acids | A |
| Mass (Da) | 6 |
| Net charge | +5 |
| Isoelectric point | 10.47 |
| Basic residues | 6 |
| Acidic residues | 1 |
| Hydrophobic residues | 35 |
| Polar residues | 22 |
| Aliphatic residues | 20 |
| Tiny residues | 25 |
| Boman Index | 42.63 |
| Hydropathy Index | 0.953 |
| Aliphatic Index | 134.38 |
| Instability Index | 9.52 (stable) |
| Half Life |
Mammalian : 5.5 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 9970 M-1 cm-1 |
| Absorbance 280nm | 158.25 |
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