Bacteriocin: Thurandacins A & B
Accession: BAC228
Classification
Class: Unclassified
Genetics
Gene: ThuA
Producer and target organisms
Producer Organism: Bacillus thuringiensis serovar andalousiensis BGSC 4AW1 [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Target organismsUnavailable data
Description
Thurandacins A and B both have an S-linked glucose moiety; thurandacin B also contains a β-O-linked glucose unit.
Uniprot and PDB links
UniProt Entry: C3GCL2
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=BGSC 4AW1 {ECO:0000313|EMBL:EEM68348.1}.
PubMed=22645259;DOI=10.1101/gr.134437.111
Zwick M.E., Joseph S.J., Didelot X., Chen P.E., Bishop-Lilly K.A., Stewart A.C., Willner K., Nolan N., Lentz S., Thomason M.K., Sozhamannan S., Mateczun A.J., Du L., Read T.D.
"Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis.", Genome Res. 22:1512-1524(2012).
PubMed=22645259;DOI=10.1101/gr.134437.111
Zwick M.E., Joseph S.J., Didelot X., Chen P.E., Bishop-Lilly K.A., Stewart A.C., Willner K., Nolan N., Lentz S., Thomason M.K., Sozhamannan S., Mateczun A.J., Du L., Read T.D.
"Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis.", Genome Res. 22:1512-1524(2012).
PROTEIN STRUCTURE
PubMed=24325644;DOI=10.1021/ja411159k
Wang H., Oman T.J., Zhang R., Garcia De Gonzalo C.V., Zhang Q., van der Donk W.A.
"The glycosyltransferase involved in thurandacin biosynthesis catalyzes both O- and S-glycosylation.", J Am Chem Soc 2014, 136 (1):84-7.
PubMed=24325644;DOI=10.1021/ja411159k
Wang H., Oman T.J., Zhang R., Garcia De Gonzalo C.V., Zhang Q., van der Donk W.A.
"The glycosyltransferase involved in thurandacin biosynthesis catalyzes both O- and S-glycosylation.", J Am Chem Soc 2014, 136 (1):84-7.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene1028 | hypothetical protein | 501..743 | |
| BACGene1029 | Sublancin 168 processing and transport ATP-binding protein similar to ABC-type bacteriocin/lantibiotic exporters, contain an N-terminaldouble-glycine peptidase domain COG2274; similar to Sublancin 168 processing andtransport ATP-binding protein of | 836..2941 | |
| BACGene1030 | Disulfide bond formation protein A similar to thioredoxin-like protein PHA2278; similar to Disulfide bond formationprotein A of Bacillus cereus E33L UniRef RepID=Q4V266_BACCZ | 3103..3516 | |
| BACGene1031 | hypothetical protein similar to YolJ protein of root UniRef RepID=O31986_BACSU | 3535..4809 | |
| BACGene1032 | Transposase for insertion sequence element IS231B similar to Transposase for insertion sequence element IS231B of Bacillus UniRefRepID=T231B_BACTB | complement(5459..6880) | |
| BACGene1033 | DNA binding protein similar to hypothetical protein PRK05590; similar to Putative DNA bindingprotein of Bacillus thuringiensis serovar israelensis UniRef RepID=Q8KNR7_BACTI | 6947..7207 | |
| BACGene1034 | hypothetical protein similar to C-terminal half of Cry protein of Bacillus thuringiensis UniRefRepID=Q6BE08_BACTU | 7411..11184 | |
| BACGene1035 | hypothetical protein similar to motif pfam03945 | complement(12027..14372) | |
| BACGene1036 | Initiator RepB protein similar to replication protein PRK13742; similar to Initiator RepB protein ofBacillus cereus group UniRef RepID=A9VVF2_BACWK | complement(14727..15818) | |
| BACGene1037 | hypothetical protein | 16294..16407 | |
| BACGene1038 | hypothetical protein | 16561..16707 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | GIGTAQCAYF KALCYSGGSE WLGGYGGCGS TQNNCELARK YC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔42 | 42 | Thurandacins A and B.
Feature identifier = |
| Modified residue | 28 | Thur A,B: S-linked glucose moiety. | |
| Modified residue | 19 | Thur B: β-O-linked glucose unit. | |
| HELIX | 3↔14 | 12 | |
| HELIX | 32↔41 | 10 | |
| DISULFID | 7↔42 | 36 | |
| DISULFID | 14↔35 | 22 |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | DHMPV |
| Common amino acids | G |
| Mass (Da) | 4 |
| Net charge | +1 |
| Isoelectric point | 7.81 |
| Basic residues | 3 |
| Acidic residues | 2 |
| Hydrophobic residues | 10 |
| Polar residues | 25 |
| Aliphatic residues | 4 |
| Tiny residues | 16 |
| Boman Index | -32.81 |
| Hydropathy Index | -0.2 |
| Aliphatic Index | 46.67 |
| Instability Index | 6.58 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 11710 M-1 cm-1 |
| Absorbance 280nm | 285.61 |
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