Bacteriocin: Thurandacins A & B

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Bacteriocin: Thurandacins A & B

Accession: BAC228

Classification
Class: Unclassified
Genetics
Gene: ThuA
Producer and target organisms

Producer Organism: Bacillus thuringiensis serovar andalousiensis BGSC 4AW1 [Gram-positive]

Taxonomy: BacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Target organismsUnavailable data

Description
Thurandacins A and B both have an S-linked glucose moiety; thurandacin B also contains a β-O-linked glucose unit.
Uniprot and PDB links

UniProt Entry: C3GCL2

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=BGSC 4AW1 {ECO:0000313|EMBL:EEM68348.1}.
PubMed=22645259;DOI=10.1101/gr.134437.111
Zwick M.E., Joseph S.J., Didelot X., Chen P.E., Bishop-Lilly K.A., Stewart A.C., Willner K., Nolan N., Lentz S., Thomason M.K., Sozhamannan S., Mateczun A.J., Du L., Read T.D.
"Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis.", Genome Res. 22:1512-1524(2012).
PROTEIN STRUCTURE
PubMed=24325644;DOI=10.1021/ja411159k
Wang H., Oman T.J., Zhang R., Garcia De Gonzalo C.V., Zhang Q., van der Donk W.A.
"The glycosyltransferase involved in thurandacin biosynthesis catalyzes both O- and S-glycosylation.", J Am Chem Soc 2014, 136 (1):84-7.
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Gene structure
genome of Thurandacins A & B

Gene idNameDescriptionLocation
BACGene1028hypothetical protein501..743
BACGene1029Sublancin 168 processing and transport ATP-binding protein similar to ABC-type bacteriocin/lantibiotic exporters, contain an N-terminaldouble-glycine peptidase domain COG2274; similar to Sublancin 168 processing andtransport ATP-binding protein of836..2941
BACGene1030Disulfide bond formation protein A similar to thioredoxin-like protein PHA2278; similar to Disulfide bond formationprotein A of Bacillus cereus E33L UniRef RepID=Q4V266_BACCZ3103..3516
BACGene1031hypothetical protein similar to YolJ protein of root UniRef RepID=O31986_BACSU3535..4809
BACGene1032Transposase for insertion sequence element IS231B similar to Transposase for insertion sequence element IS231B of Bacillus UniRefRepID=T231B_BACTBcomplement(5459..6880)
BACGene1033DNA binding protein similar to hypothetical protein PRK05590; similar to Putative DNA bindingprotein of Bacillus thuringiensis serovar israelensis UniRef RepID=Q8KNR7_BACTI6947..7207
BACGene1034hypothetical protein similar to C-terminal half of Cry protein of Bacillus thuringiensis UniRefRepID=Q6BE08_BACTU7411..11184
BACGene1035hypothetical protein similar to motif pfam03945complement(12027..14372)
BACGene1036Initiator RepB protein similar to replication protein PRK13742; similar to Initiator RepB protein ofBacillus cereus group UniRef RepID=A9VVF2_BACWKcomplement(14727..15818)
BACGene1037hypothetical protein16294..16407
BACGene1038hypothetical protein16561..16707
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 GIGTAQCAYF KALCYSGGSE WLGGYGGCGS TQNNCELARK YC

Wheel representation
wheel representation of Thurandacins A & B
Protein sequence annotations
Features of Thurandacins A & B

SC
FeaturePosition(s)LengthDescription
CHAIN1↔4242
Thurandacins A and B.
Feature identifier =
Modified residue28Thur A,B: S-linked glucose moiety.
Modified residue19Thur B: β-O-linked glucose unit.
HELIX3↔1412
HELIX32↔4110
DISULFID7↔4236
DISULFID14↔3522
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids DHMPV
Common amino acids G
Mass (Da) 4
Net charge +1
Isoelectric point 7.81
Basic residues 3
Acidic residues 2
Hydrophobic residues 10
Polar residues 25
Aliphatic residues 4
Tiny residues 16
Boman Index -32.81
Hydropathy Index -0.2
Aliphatic Index 46.67
Instability Index 6.58 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 11710 M-1 cm-1
Absorbance 280nm 285.61

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