Bacteriocin: Elgicins
Accession: BAC227
Classification
Class: Lantibiotic IA
Genetics
Gene: elgA
Producer and target organisms
Producer Organism: Paenibacillus elgii B69 [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
Target organismsStaphylococcus epidermidis CMCC 26069 - Staphylococcus aureus ATCC 43300 - Pseudomonas aeruginosa ATCC 27853 - Escherichia coli ATCC 35218 - Proteus vulgaris CMCC 49027 -
NOTE: not active against Staphylococcus aureus ATCC 25923 - Bacillus subtilis CGMCC 11470
Description
elgA gene encodes the prepeptide of the elgicins, with a length of 64 amino acids. The origin of elgicins from identical pre-peptides by peptide cleavage and the removal of one amino acid from each corresponding N-terminus. ElgA is cleaved at four sites A20-N21, N21-L22, L22-G23, and G23-N24, resulting in the production of elgicin C, B, AII, and AI, respectively.
Uniprot and PDB links
UniProt Entry: I1YCV0
PDB EntryUnknown
SEQUENCE CHARACTERISATION
PubMed=22443157;DOI=10.1186/1471-2180-12-45
Teng Y., Zhao W., Qian C., Li O., Zhu L., Wu X.
"Gene cluster analysis for the biosynthesis of elgicins, novel lantibiotics produced by Paenibacillus elgii B69.", BMC Microbiol 2012, 12:45.
PubMed=22443157;DOI=10.1186/1471-2180-12-45
Teng Y., Zhao W., Qian C., Li O., Zhu L., Wu X.
"Gene cluster analysis for the biosynthesis of elgicins, novel lantibiotics produced by Paenibacillus elgii B69.", BMC Microbiol 2012, 12:45.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene1023 | elgT1 | ElgT1 ABC-type transport protein | 1..1791 |
| BACGene1024 | elgC | ElgC lantibiotic synthetase | complement(2199..3563) |
| BACGene1025 | elgT2 | ElgT2 ABC-type transport protein | complement(3602..5479) |
| BACGene1026 | elgB | ElgB lantibiotic dehydratase | complement(5476..8697) |
| BACGene1027 | elgA | elgicins | complement(8805..8999) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | ANLGNYTSQC YSSQCYSSKC YSDSCYSSNC YTGRHMCGYT HGYSC |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔45 | 45 | Elgicin C.
Feature identifier = |
| Cleavage | 1 | Cleavage site for Egicin B | |
| Cleavage | 2 | Cleavage site for Egicin AII | |
| Cleavage | 3 | Cleavage site for Egicin AI |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | HQ |
| Common amino acids | S |
| Mass (Da) | 279.97 |
| Net charge | +1 |
| Isoelectric point | 7.8 |
| Basic residues | -2 |
| Acidic residues | -3 |
| Hydrophobic residues | -10 |
| Polar residues | 23 |
| Aliphatic residues | 1 |
| Tiny residues | 15 |
| Boman Index | -23.78 |
| Hydropathy Index | -0.283 |
| Aliphatic Index | -39.36 |
| Instability Index | 33.25 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | -1810 M-1 cm-1 |
| Absorbance 280nm | -39.35 |
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