Bacteriocin: BacCH91
Accession: BAC226
Classification
Class: Lantibiotic IA
Genetics
Gene: BacCH91
Producer and target organisms
Producer Organism: Staphylococcus aureus CH-91 DSM 26258 [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus
Target organismsMicrococcus luteus (MIC=2.5 nM) - staphylococcus staphylococcus (MIC range 1.3-6.0 μM) - Lactococcus lactis (MIC=100 μM) -
Note: ineffective against tested Gram-negative strains at maximal concentration (100 µM)
Description
It is a thermostable peptide that is highly resistant to cleavage by both prokaryotic and eukaryotic peptidases.
The encoding gene (BacCH91) occurred in two allelic variants distinguishable in the restriction fragment length polymorphism assay. Variant I, identified in S. aureus CH-91, dominated in S. aureus strains of poultry origin, although strains with variant II were also identified in this group. S. aureus strains of human origin were characterized exclusively by variant II.
The encoding gene (BacCH91) occurred in two allelic variants distinguishable in the restriction fragment length polymorphism assay. Variant I, identified in S. aureus CH-91, dominated in S. aureus strains of poultry origin, although strains with variant II were also identified in this group. S. aureus strains of human origin were characterized exclusively by variant II.
Uniprot and PDB links
UniProt Entry: I6XG59
PDB EntryUnknown
STRUCTURE AND ACTIVITY
PubMed=23196985;DOI=10.1007/s00253-012-4578-y
Wladyka B., Wielebska K., Wloka M., Bochenska O., Dubin G., Dubin A., Mak P.
"Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91.", Appl Microbiol Biotechnol 2013, 97 (16):7229-39.
PubMed=23196985;DOI=10.1007/s00253-012-4578-y
Wladyka B., Wielebska K., Wloka M., Bochenska O., Dubin G., Dubin A., Mak P.
"Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91.", Appl Microbiol Biotechnol 2013, 97 (16):7229-39.
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Protein sequence
| ........10 ........20 ........30 | | | ITSFIGCTPG CGKTGSFNSF CC |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | ADEHLMQRVWY |
| Common amino acids | GC |
| Mass (Da) | 2 |
| Net charge | +1 |
| Isoelectric point | 7.92 |
| Basic residues | 1 |
| Acidic residues | 0 |
| Hydrophobic residues | 5 |
| Polar residues | 15 |
| Aliphatic residues | 2 |
| Tiny residues | 7 |
| Boman Index | -2.44 |
| Hydropathy Index | 0.559 |
| Aliphatic Index | 35.45 |
| Instability Index | 16.65 (stable) |
| Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 250 M-1 cm-1 |
| Absorbance 280nm | 11.9 |
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