Bacteriocin: LsbB
Accession: BAC223
Classification
Class: class II leaderless
Genetics
Gene: lsbB
Producer and target organisms
Producer Organism: Lactococcus lactis subsp. lactis (Streptococcus lactis) [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q7X2B5
PDB Entry2MLU resolved by NMR 2MLV resolved by NMR
NUCLEOTIDE SEQUENCE, STRAIN=BGMN1-5; PLASMID=pMN5.
PubMed=12801935;DOI=10.1074/jbc.M211100200
Gajic O., Buist G., Kojic M., Topisirovic L., Kuipers O.P., Kok J.
"Novel mechanism of bacteriocin secretion and immunity carried out by lactococcal multidrug resistance proteins.", J. Biol. Chem. 278:34291-34298(2003).
PubMed=12801935;DOI=10.1074/jbc.M211100200
Gajic O., Buist G., Kojic M., Topisirovic L., Kuipers O.P., Kok J.
"Novel mechanism of bacteriocin secretion and immunity carried out by lactococcal multidrug resistance proteins.", J. Biol. Chem. 278:34291-34298(2003).
PEPTIDE STRUCTURE
PubMed=24993828;DOI=10.1074/jbc.M114.579698
Ovchinnikov K.V., Kristiansen P.E., Uzelac G., Topisirovic L., Kojic M., Nissen-Meyer J., Nes I.F., Diep D.B.
"Defining the structure and receptor binding domain of the leaderless bacteriocin LsbB.", J Biol Chem 2014, ():.
PubMed=24993828;DOI=10.1074/jbc.M114.579698
Ovchinnikov K.V., Kristiansen P.E., Uzelac G., Topisirovic L., Kojic M., Nissen-Meyer J., Nes I.F., Diep D.B.
"Defining the structure and receptor binding domain of the leaderless bacteriocin LsbB.", J Biol Chem 2014, ():.
MECHANISM OF ACTION
PubMed=24123824;DOI=10.1128/JB.00859-13
Uzelac G., Kojic M., Lozo J., Aleksandrzak-Piekarczyk T., Gabrielsen C., Kristensen T., Nes I.F., Diep D.B., Topisirovic L.
"A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5.", J Bacteriol 2013, 195 (24):5614-21.
PubMed=24123824;DOI=10.1128/JB.00859-13
Uzelac G., Kojic M., Lozo J., Aleksandrzak-Piekarczyk T., Gabrielsen C., Kristensen T., Nes I.F., Diep D.B., Topisirovic L.
"A Zn-dependent metallopeptidase is responsible for sensitivity to LsbB, a class II leaderless bacteriocin of Lactococcus lactis subsp. lactis BGMN1-5.", J Bacteriol 2013, 195 (24):5614-21.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene1019 | lsbA | LsbA bacteriocin | 221..424 |
| BACGene1020 | lsbB | LsbB bacteriocin | complement(464..556) |
| BACGene1021 | lmrB | LmrB multidrug resistance protein; involved in secretion of and immunity againstbacteriocins LsbA and LsbB | 653..2356 |
| BACGene1022 | repA | RepA replication protein | 4507..5457 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | MKTILRFVAG YDIASHKKKT GGYPWERGKA |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔30 | 30 | Peptide LsdB.
Feature identifier = |
| HELIX | 4↔17 | 14 |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | CNQ |
| Common amino acids | K |
| Mass (Da) | 3 |
| Net charge | +6 |
| Isoelectric point | 10.75 |
| Basic residues | 8 |
| Acidic residues | 2 |
| Hydrophobic residues | 9 |
| Polar residues | 9 |
| Aliphatic residues | 4 |
| Tiny residues | 8 |
| Boman Index | -50.95 |
| Hydropathy Index | -0.683 |
| Aliphatic Index | 58.67 |
| Instability Index | 31.41 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 8480 M-1 cm-1 |
| Absorbance 280nm | 292.41 |
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