Bacteriocin: NAI-107
Accession: BAC222
Classification
Class: Lantibiotic
Genetics
Gene: mlbA
Producer and target organisms
Producer Organism: Microbispora sp. (strain 107891) [Gram-positive]
Taxonomy: Bacteria
ActinobacteriaActinobacteridaeActinomycetalesStreptosporangineaeStreptosporangiaceaeMicrobispora
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: P85065
PDB Entry2MH5 resolved by NMR
NUCLEOTIDE SEQUENCE, STRAIN=ATCC PTA-5024.
PubMed=24459268;
Sosio M., Gallo G., Pozzi R., Serina S., Monciardini P., Bera A., Stegmann E., Weber T.
"Draft Genome Sequence of the Microbispora sp. Strain ATCC-PTA-5024, Producing the Lantibiotic NAI-107.", Genome Announc. 2:e01198-13(2014).
PubMed=24459268;
Sosio M., Gallo G., Pozzi R., Serina S., Monciardini P., Bera A., Stegmann E., Weber T.
"Draft Genome Sequence of the Microbispora sp. Strain ATCC-PTA-5024, Producing the Lantibiotic NAI-107.", Genome Announc. 2:e01198-13(2014).
[Ref. 2]
PROTEIN SEQUENCE, FUNCTION, POST-TRANSLATIONAL MODIFICATIONS, AND MASS SPECTROMETRY.
Lazzarini A., Gastaldo L., Candiani G., Ciciliato I., Losi D., Marinelli F., Selva E., Parenti F.
"Antibiotic 107891, its factors A1 and A2, pharmaceutically acceptable salts and compositions, and use thereof.", Patent number WO2005014628, 17-FEB-2005.
Lazzarini A., Gastaldo L., Candiani G., Ciciliato I., Losi D., Marinelli F., Selva E., Parenti F.
"Antibiotic 107891, its factors A1 and A2, pharmaceutically acceptable salts and compositions, and use thereof.", Patent number WO2005014628, 17-FEB-2005.
PROTEIN STRUCTURE AND MECHANISM OF ACTION
PubMed=24627484;DOI=10.1074/jbc.M113.537449
Munch D., Muller A., Schneider T., Kohl B., Wenzel M., Bandow J.E., Maffioli S., Sosio M., Donadio S., Wimmer R., Sahl H.
"The lantibiotic NAI-107 binds to bactoprenol-bound cell wall precursors and impairs membrane functions.", J Biol Chem 2014, 289 (17):12063-76.
PubMed=24627484;DOI=10.1074/jbc.M113.537449
Munch D., Muller A., Schneider T., Kohl B., Wenzel M., Bandow J.E., Maffioli S., Sosio M., Donadio S., Wimmer R., Sahl H.
"The lantibiotic NAI-107 binds to bactoprenol-bound cell wall precursors and impairs membrane functions.", J Biol Chem 2014, 289 (17):12063-76.
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Protein sequence
| ........10 ........20 ........30 | | | VTSWSLCTPG CTSPGGGSNC SFCC |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔24 | 24 | Lantibiotic 107891. Feature identifier = PRO_0000271410.
|
| Modified residue | 2 | (E)-2,3-didehydrobutyrine. | |
| Modified residue | 4 | 6'-chlorotryptophan. | |
| Modified residue | 5 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 14 | 3,4-dihydroxyproline; in form A1. | |
| Modified residue | 14 | 4-hydroxyproline; in form A2. | |
| Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 13↔20 | 8 | Lanthionine (Ser-Cys). |
| Cross-link | 18↔23 | 6 | Lanthionine (Ser-Cys). |
| Cross-link | 21↔24 | 4 | S-(2-aminovinyl)-D-cysteine (Ser-Cys). |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | ADEHIKMQRY |
| Common amino acids | SC |
| Mass (Da) | 2 |
| Net charge | 0 |
| Isoelectric point | 5.66 |
| Basic residues | 0 |
| Acidic residues | 0 |
| Hydrophobic residues | 4 |
| Polar residues | 18 |
| Aliphatic residues | 2 |
| Tiny residues | 9 |
| Boman Index | -6.92 |
| Hydropathy Index | 0.333 |
| Aliphatic Index | 28.33 |
| Instability Index | 60.6 (unstable) |
| Half Life |
Mammalian : 100 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 5750 M-1 cm-1 |
| Absorbance 280nm | 250 |
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