Bacteriocin: Enterocin 7B

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Bacteriocin: Enterocin 7B

Accession: BAC220

Classification
Class: Unclassified
Genetics
Gene: mr10B; Synonyms=entNB
Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]

Taxonomy: BacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Target organismsUnavailable data

NUCLEOTIDE SEQUENCE, STRAIN=MRR 10-3.
PubMed=16751538;DOI=10.1128/AEM.02940-05
Martin-Platero A.M., Valdivia E., Ruiz-Rodriguez M., Soler J.J., Martin-Vivaldi M., Maqueda M., Martinez-Bueno M.
"Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops).", Appl. Environ. Microbiol. 72:4245-4249(2006).
NUCLEOTIDE SEQUENCE, STRAIN=N1-33.
Hata T., Alemu M., Kobayashi M., Tanaka R., Sato T., Suzuki C., Nitisinprasert S., Ohmomo S.
"Identification of enterocin N produced by Enterococcus faecalis N1-33 isolated from fermented vegetables in Thailand and its application to ready-to-eat foods as a biopreservative.", Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=DBH18.
Jimenez J.J., Borrero J., Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB.", Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
3D STRUCTURE
PubMed=23725536;DOI=10.1021/bi400359z
Lohans C.T., Towle K.M., Miskolzie M., McKay R.T., van Belkum M.J., McMullen L.M., Vederas J.C.
"Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide", Biochemistry 52: 3987-3994 (2013).
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End Note
Reference Manager
BibTeX
Gene structure
genome of Enterocin 7B

Gene idNameDescriptionLocation
BACGene1007hypothetical protein orf11..1830
BACGene1008enterocin JSA leader-less enterocin; entJSA2370..2504
BACGene1009enterocin JSB leader-less enterocin; entJSB2525..2656
BACGene1010hypothetical protein orf42877..3146
BACGene1011hypothetical protein orf53147..3416
BACGene1012hypothetical protein orf63420..3839
BACGene1013hypothetical protein orf73836..5212
BACGene1014hypothetical protein orf85222..5713
BACGene1015hypothetical protein orf95706..6926
BACGene1016hypothetical protein orf106923..7606
BACGene1017hypothetical protein orf117603..8802
BACGene1018hypothetical protein orf129758..11383
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 MGAIAKLVAK FGWPFIKKFY KQIMQFIGQG WTIDQIEKWL KRH

Wheel representation
wheel representation of Enterocin 7B
3D Structure
Protein sequence annotations
Features of Enterocin 7B


FeaturePosition(s)LengthDescription
PEPTIDE1↔4343
Enterocin 7B.
Feature identifier =
HELIX4↔85
HELIX13↔164
HELIX20↔2910
HELIX33↔408
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids CNS
Common amino acids K
Mass (Da) 5183.64
Net charge +7
Isoelectric point 10.95
Basic residues 9
Acidic residues 2
Hydrophobic residues 19
Polar residues 6
Aliphatic residues 9
Tiny residues 7
Boman Index -22.63
Hydropathy Index -0.109
Aliphatic Index 86.28
Instability Index 43.32 (unstable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 17990 M-1 cm-1
Absorbance 280nm 428.33

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