Bacteriocin: Enterocin 7B
Accession: BAC220
Classification
Class: Unclassified
Genetics
Gene: mr10B; Synonyms=entNB
Producer and target organisms
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacilli
Lactobacillales
Enterococcaceae
Enterococcus
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: Q1A2D2
PDB Entry2M60 resolved by NMR
NUCLEOTIDE SEQUENCE, STRAIN=MRR 10-3.
PubMed=16751538;DOI=10.1128/AEM.02940-05
Martin-Platero A.M., Valdivia E., Ruiz-Rodriguez M., Soler J.J., Martin-Vivaldi M., Maqueda M., Martinez-Bueno M.
"Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops).", Appl. Environ. Microbiol. 72:4245-4249(2006).
PubMed=16751538;DOI=10.1128/AEM.02940-05
Martin-Platero A.M., Valdivia E., Ruiz-Rodriguez M., Soler J.J., Martin-Vivaldi M., Maqueda M., Martinez-Bueno M.
"Characterization of antimicrobial substances produced by Enterococcus faecalis MRR 10-3, isolated from the uropygial gland of the hoopoe (Upupa epops).", Appl. Environ. Microbiol. 72:4245-4249(2006).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=N1-33.
Hata T., Alemu M., Kobayashi M., Tanaka R., Sato T., Suzuki C., Nitisinprasert S., Ohmomo S.
"Identification of enterocin N produced by Enterococcus faecalis N1-33 isolated from fermented vegetables in Thailand and its application to ready-to-eat foods as a biopreservative.", Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
Hata T., Alemu M., Kobayashi M., Tanaka R., Sato T., Suzuki C., Nitisinprasert S., Ohmomo S.
"Identification of enterocin N produced by Enterococcus faecalis N1-33 isolated from fermented vegetables in Thailand and its application to ready-to-eat foods as a biopreservative.", Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
[Ref. 3]
NUCLEOTIDE SEQUENCE, STRAIN=DBH18.
Jimenez J.J., Borrero J., Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB.", Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
Jimenez J.J., Borrero J., Sanchez J., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Enterococcus faecalis DBH18, isolated from Mallard ducks (Anas platyrhynchos), encodes the bacteriocin-producing genes ef1097, entL50A-entL50B, and the entL50A-entL50B-like genes termed entJSA and entJSB.", Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
3D STRUCTURE
PubMed=23725536;DOI=10.1021/bi400359z
Lohans C.T., Towle K.M., Miskolzie M., McKay R.T., van Belkum M.J., McMullen L.M., Vederas J.C.
"Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide", Biochemistry 52: 3987-3994 (2013).
PubMed=23725536;DOI=10.1021/bi400359z
Lohans C.T., Towle K.M., Miskolzie M., McKay R.T., van Belkum M.J., McMullen L.M., Vederas J.C.
"Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide", Biochemistry 52: 3987-3994 (2013).
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Gene structure
Gene id | Name | Description | Location |
BACGene1007 | hypothetical protein orf1 | 1..1830 | |
BACGene1008 | enterocin JSA leader-less enterocin; entJSA | 2370..2504 | |
BACGene1009 | enterocin JSB leader-less enterocin; entJSB | 2525..2656 | |
BACGene1010 | hypothetical protein orf4 | 2877..3146 | |
BACGene1011 | hypothetical protein orf5 | 3147..3416 | |
BACGene1012 | hypothetical protein orf6 | 3420..3839 | |
BACGene1013 | hypothetical protein orf7 | 3836..5212 | |
BACGene1014 | hypothetical protein orf8 | 5222..5713 | |
BACGene1015 | hypothetical protein orf9 | 5706..6926 | |
BACGene1016 | hypothetical protein orf10 | 6923..7606 | |
BACGene1017 | hypothetical protein orf11 | 7603..8802 | |
BACGene1018 | hypothetical protein orf12 | 9758..11383 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | MGAIAKLVAK FGWPFIKKFY KQIMQFIGQG WTIDQIEKWL KRH |
Wheel representation
3D Structure
Protein sequence annotations
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔43 | 43 | Enterocin 7B.
Feature identifier = |
HELIX | 4↔8 | 5 | |
HELIX | 13↔16 | 4 | |
HELIX | 20↔29 | 10 | |
HELIX | 33↔40 | 8 |
Composition
Hydrophobicity
Composition
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | CNS |
Common amino acids | K |
Mass (Da) | 5183.64 |
Net charge | +7 |
Isoelectric point | 10.95 |
Basic residues | 9 |
Acidic residues | 2 |
Hydrophobic residues | 19 |
Polar residues | 6 |
Aliphatic residues | 9 |
Tiny residues | 7 |
Boman Index | -22.63 |
Hydropathy Index | -0.109 |
Aliphatic Index | 86.28 |
Instability Index | 43.32 (unstable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 17990 M-1 cm-1 |
Absorbance 280nm | 428.33 |
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