Bacteriocin: Cypemycin

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Bacteriocin: Cypemycin

Accession: BAC218

Classification
Class: Unclassified
Genetics
Gene: cypA
Producer and target organisms
Description
FUNCTION:
- Antibiotic peptide structurally related to lantibiotics.
- Active against M.luteus (MIC=0.2 ug/ml). Has cytotoxic activity against mouse P388 leukemia cells (IC(50)=1.3 ug/ml).

SUBCELLULAR LOCATION:
- Secreted. Secreted, extracellular space.

PTM:
- Maturation involves the enzymatic conversion of Cys-61 into 2,3-didehydroalanine followed by formation of a thioether bond with Cys-64. The C-terminal meso-lanthionine then undergoes decarboxylation.
- Dimethylated at the N-terminus. Dimethylation is required for antibiotic activity.
- The configuration of reformed Cys-61 as the D form was attributed by similarity to related structures.

SIMILARITY:
- Belongs to the linaridin family.
Uniprot and PDB links

UniProt Entry: E5KIB6

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 45-60, FUNCTION, MASS SPECTROMETRY, AND METHYLATION AT ALA-43, STRAIN=OH-4156.
PubMed=20805503;DOI=10.1073/pnas.1008608107
Claesen J., Bibb M.
"Genome mining and genetic analysis of cypemycin biosynthesis reveal an unusual class of posttranslationally modified peptides.", Proc. Natl. Acad. Sci. U.S.A. 107:16297-16302(2010).
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Gene structure
genome of Cypemycin

Gene idNameDescriptionLocation
BACGene998putative regulatory protein orf1complement(57..3308)
BACGene999cypemycin structural protein cypA3882..4076
BACGene1000hypothetical protein cypH; contains N-terminal HTTM domain and C-terminal alpha/beta hydrolase fold4191..5945
BACGene1001unknown cypL5935..6495
BACGene1002HFCD family decarboxylase cypD6492..7064
BACGene1003SAM-dependent methyltransferase cypM7057..7794
BACGene1004ABC transporter ATP binding subunit cypT7791..8423
BACGene1005putative transmembrane pore cypP; contains 12 transmembrane helices8420..9970
BACGene1006DUF255 family protein cypI10053..12074
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 ATPATPTVAQ FVIQGSTICL VC

Wheel representation
wheel representation of Cypemycin
Protein sequence annotations
Features of Cypemycin

ATTTITI
FeaturePosition(s)LengthDescription
PEPTIDE1↔2222
Cypemycin.
Feature identifier = PRO_0000408758.
Modified residue1N,N-dimethylalanine.
Modified residue2(E)-2,3-didehydrobutyrine.
Modified residue5(E)-2,3-didehydrobutyrine.
Modified residue7(E)-2,3-didehydrobutyrine.
Modified residue13L-allo-isoleucine.
Modified residue17(E)-2,3-didehydrobutyrine.
Modified residue18L-allo-isoleucine.
Cross-link19↔224S-(2-aminovinyl)-D-cysteine (Cys-Cys).
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids DEHKMNRWY
Common amino acids T
Mass (Da) 2
Net charge 0
Isoelectric point 5.84
Basic residues 0
Acidic residues 0
Hydrophobic residues 10
Polar residues 8
Aliphatic residues 6
Tiny residues 5
Boman Index 14.03
Hydropathy Index 1.109
Aliphatic Index 106.36
Instability Index 23.63 (stable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 125 M-1 cm-1
Absorbance 280nm 5.95

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