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Bacteriocin: Thuricin CD alpha

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Bacteriocin: Thuricin CD alpha

Accession: BAC216
Classification
Class: two-peptide lantibiotic
Genetics
Gene: Unidentified
Producer and target organisms

Producer Organism: Bacillus thuringiensis DPC 6431 [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Target organismsactive against Bacillus cereus NCIMB 700577 7(+++) - Bacillus cereus NCIMB 700578 8(+++) - Bacillus firmus LMG 7125T T(++++) - Bacillus mycoides DPC 6335 5(+++) - Bacillus thuringiensis LMG 7138 8(+++) - Clostridium difficile ATCC 600 0(++++) - Clostridium difficile ATCC 43594 4(++++) - Clostridium difficile R027 NCTC 13366 6(++++) - Clostridium difficile 6220 0(++++) - Clostridium difficile 6221 1(++++) - Clostridium difficile 6219 9(++++) - Clostridium difficile 6350 0(++++) - Clostridium difficile 6351 1(++++) - Clostridium difficile IBS 16 6(++++) - Clostridium difficile Cr 15 5(++++) - Clostridium difficile UC 38 8(++++) - Clostridium difficile UC 38 8(++++) - Clostridium difficile CR 79 9(++++) - Clostridium difficile UC 47 7(++++) - Clostridium difficile CR11 1(++++) - Clostridium difficile UC 29 9(++++) - Clostridium difficile CR 02 2(++++) - Clostridium difficile UC 59 9(++++) - Clostridium difficile UC 27 7(++++) - Clostridium difficile H 76 6(++++) - Clostridium difficile BS 47 7(++++) - Clostridium histolyticum NCIMB 503 3(+) - Clostridium indolis NCIMB 9731 1(++++) - Clostridium lituseburense NCIMB 10637 7(+++) - Clostridium tyrobutyricum NCIMB 8243 3(+++) - Lactobacillus crispatus LMG 9479T T(+) - Lactobacillus fermentum LMG 6902 2(+++) - Lactobacillus johnsonii DSM 10533T T(+) - Lactococcus lactis DPC 3157 7(+) - Listeria innocua DPC 3572 2(+++) - Listeria monocytogenes DPC 1768 8(+++) - Listeria monocytogenes DPC 3437 7(+++) - Listeria monocytogenes Scott A A(+++) -

NOTE: (Diameter of the zone: Zones of size <=9 mm were designated +; of 10-15 mm were designated ++; of 16-21 mm were designated +++; of >= 22 mm were designated ++++). -

NOTE: not active against Bacillus coagulans LMG 6326 NOTE: not active against Bacillus coagulans LMG 6326T - Bacillus subtilis LMG 8198 - Bacillus subtilis DPC 3344 - Bacillus thuringiensis IBS 14 - Bacteroides fragilis LMG 10263 - Bifidobacterium adolescensis DPC 6169 - Bifidobacterium animalis DPC 5420 - Bifidobacterium breve DPC 6166 - Bifidobacterium breve BB12 - Bifidobacterium longum DSMZ 20097 - Bifidobacterium merycicum DSMZ 6493 - Bifidobacterium pseudolongum DSMZ 20092 - Campylobacter jejuni CI 120 - Clostridium sporogenes NCIMB 9584 - Enterobacter sakazaki ATCC 12869 - Enterobacter sakazaki NCTC 8155 - Enterococcus casseliflavus LMG 10745T - Enterococcus durans LMG 10746T - Enterococcus faecalis LMG 7973T - Enterococcus faecium LMG 7973T - Enterococcus hirae LMG 6399T - Eschericia coli 3786 - Lactobacillus acidopuilus NCDO 1697 - Lactobacillus bulgaricis LMG 6901t - Lactobacillus casei ATCC 334 - Lactobacillus curvatus LMG 12009 - Lactobacillus gallinarum LMG 9435T - Lactobacillus helveticus LH1 - Lactobacillus paracasei 338 - Lactobacillus reuteri NCIMB 11951 - Lactobacillus rhamnosus GG - Lactobacillus ruminis DSM 20403T - Lactobacillus salivarius UCC 118 - Lactococcus lactis HP - Leuconostoc DPC 139 - Leuconostoc DPC 240 - Micrococcus luteus DPC 6275 - Micrococcus luteus LMG 3293 - Pediococcus pentasaceus LMG 11488 - Propionibacterium avidium LMG - Proprionibacterium acne LMG - Propionibacterium jensenii NCFB 565 - Propionibacterium jensenii NCFB 850T - Pseudomonas putida ATCC 33015 - Pseudomonas putida ATCC 17522 - Salmonella enterica serovar Typhimurium DPC 6046 - Salmonella enterica serovar Derby DPC 6049 - Staphylococcus aureus ATCC 25923 - Staphylococcus aureus DPC 3767 - Staphylococcus saphrophyticus DPC 6289 - Streptococcus algalactiae LMG 14694t - Streptococcus bovis DPC 5244 - Streptococcus dysgalactia DPC 5345 - Steptococcus mutans 6159 DPC 6159 - Steptococcus mutans 6155 DPC 6155 - Steptococcus mutans 6143 DPC 6143.}

Description
STRUCTURE:
Thuricin CD is a two-component antimicrobial peptide system with sulfur to α-carbon linkages. NMR analysis of Trn-α and Trn-β sheds light on the nature of the posttranslational modifications at residues 21, 25, and 28.
Uniprot and PDB links

UniProt Entry: C2TQ80

PDB Entry2L9X resolved by NMR

[Ref. 1] | View abstract | Export citation
STRUCTURE BY NMR OF 18-47.
PubMed=21526839;DOI=10.1021/ja201802f
Sit C.S., McKay R.T., Hill C., Ross R.P., Vederas J.C.
"The 3D structure of thuricin CD, a two-component bacteriocin with cysteine sulfur to ?-carbon cross-links.", J. Am. Chem. Soc. 133:7680-7683(2011).
[Ref. 2] | View abstract | Export citation
NUCLEOTIDE SEQUENCE, STRAIN=95/8201.
PubMed=22645259;DOI=10.1101/gr.134437.111
Zwick M.E., Joseph S.J., Didelot X., Chen P.E., Bishop-Lilly K.A., Stewart A.C., Willner K., Nolan N., Lentz S., Thomason M.K., Sozhamannan S., Mateczun A.J., Du L., Read T.D.
"Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis.", Genome Res. 22:1512-1524(2012).
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End Note
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Gene structure
genome of Thuricin CD alpha

Gene idNameDescriptionLocation
BACGene964ABC transporter, permease protein similar to ABC-type transport system involved in multi-copper enzyme maturation,permease component COG1277; similar to Putative ABC transporter, permeaseprotein of Clostridium difficile UniRef RepID=Q187Z2_CLOD649..564
BACGene965hypothetical protein609..758
BACGene966hypothetical protein806..949
BACGene967Radical SAM domain protein similar to Arylsulfatase regulator (Fe-S oxidoreductase) COG0641; similar toRadical SAM domain protein of Petrotoga mobilis SJ95 UniRef RepID=A9BJJ9_PETMO1081..2580
BACGene968Radical SAM domain protein similar to Arylsulfatase regulator (Fe-S oxidoreductase) COG0641; similar toRadical SAM domain protein of Thermosinus carboxydivorans Nor1 UniRefRepID=A1HP80_9FIRM2595..3821
BACGene969Carboxyl-terminal protease similar to Carboxyl-terminal protease of Thermotogaceae UniRefRepID=A8F4J5_THELT4020..5045
BACGene970Recombinase similar to Recombinase of Bacillaceae UniRef RepID=A7GL27_BACCNcomplement(5669..6040)
BACGene971IS3-family transposase, OrfB similar to IS3-family transposase, OrfB of root UniRef RepID=A3I710_9BACI6143..6289
BACGene972hypothetical protein similar to Putative uncharacterized protein of Bacillus cereus G9241 UniRefRepID=Q4MUZ2_BACCEcomplement(6316..6765)
BACGene973hypothetical protein similar to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/S1 RNA-bindingdomain protein PRK00087; similar to Possible ribosomal protein S1 of Bacilluscereus UniRef RepID=Q4V1L4_BACCZ6983..7894
BACGene974hypothetical protein similar to Putative uncharacterized protein of Bacillus cereus UniRefRepID=Q4MUZ4_BACCE7894..8865
BACGene975hypothetical protein9203..9535
BACGene976hypothetical protein similar to Putative uncharacterized protein of Listeria monocytogenes str. 1/2aF6854 UniRef RepID=Q4ESH9_LISMO9601..10020
BACGene977DEAD/DEAH box helicase domain protein similar to Superfamily II helicase COG1204; similar to DEAD/DEAH box helicasedomain protein of Listeria monocytogenes str. 1/2a F6854 UniRefRepID=Q4ESI0_LISMO10124..13372
BACGene978hypothetical protein similar to Putative uncharacterized protein of Desulfitobacterium hafniense Y51UniRef RepID=Q24ZE7_DESHYcomplement(13529..14044)
BACGene979hypothetical proteincomplement(14068..14370)
BACGene980hypothetical proteincomplement(14446..15459)
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 GNAACVIGCI GSCVISEGIG SLVGTAFTLG
Wheel representation
wheel representation of Thuricin CD alpha
3D Structure
Protein sequence annotations
Features of Thuricin CD alpha

STT
FeaturePosition(s)LengthDescription
PEPTIDE1↔3030
Lantibiotic Thuricin CD Trn-α.
Feature identifier =
Modified residue21dehydro-Ser.
Modified residue25dehydro-Thr.
Modified residue28dehydro-Thr.
Cross-link5↔2824α-carbon thioether (Cys-Ser).
Cross-link9↔2517α-carbon thioether (Cys-Thr).
Cross-link13↔219α-carbon thioether (Cys-Thr).
HELIX3↔97
HELIX12↔165
HELIX21↔244
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids DHKMPQRWY
Common amino acids G
Mass (Da) 2763
Net charge -1
Isoelectric point 3.85
Basic residues 0
Acidic residues 1
Hydrophobic residues 13
Polar residues 16
Aliphatic residues 9
Tiny residues 13
Boman Index 31.68
Hydropathy Index 1.343
Aliphatic Index 117
Instability Index 25.25 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 125 M-1 cm-1
Absorbance 280nm 4.31

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