Bacteriocin: Thuricin CD alpha
Accession: BAC216
Producer Organism: Bacillus thuringiensis DPC 6431 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacillales
Bacillaceae
Bacillus
Bacillus cereus group
Target organismsactive against Bacillus cereus NCIMB 700577 7(+++) - Bacillus cereus NCIMB 700578 8(+++) - Bacillus firmus LMG 7125T T(++++) - Bacillus mycoides DPC 6335 5(+++) - Bacillus thuringiensis LMG 7138 8(+++) - Clostridium difficile ATCC 600 0(++++) - Clostridium difficile ATCC 43594 4(++++) - Clostridium difficile R027 NCTC 13366 6(++++) - Clostridium difficile 6220 0(++++) - Clostridium difficile 6221 1(++++) - Clostridium difficile 6219 9(++++) - Clostridium difficile 6350 0(++++) - Clostridium difficile 6351 1(++++) - Clostridium difficile IBS 16 6(++++) - Clostridium difficile Cr 15 5(++++) - Clostridium difficile UC 38 8(++++) - Clostridium difficile UC 38 8(++++) - Clostridium difficile CR 79 9(++++) - Clostridium difficile UC 47 7(++++) - Clostridium difficile CR11 1(++++) - Clostridium difficile UC 29 9(++++) - Clostridium difficile CR 02 2(++++) - Clostridium difficile UC 59 9(++++) - Clostridium difficile UC 27 7(++++) - Clostridium difficile H 76 6(++++) - Clostridium difficile BS 47 7(++++) - Clostridium histolyticum NCIMB 503 3(+) - Clostridium indolis NCIMB 9731 1(++++) - Clostridium lituseburense NCIMB 10637 7(+++) - Clostridium tyrobutyricum NCIMB 8243 3(+++) - Lactobacillus crispatus LMG 9479T T(+) - Lactobacillus fermentum LMG 6902 2(+++) - Lactobacillus johnsonii DSM 10533T T(+) - Lactococcus lactis DPC 3157 7(+) - Listeria innocua DPC 3572 2(+++) - Listeria monocytogenes DPC 1768 8(+++) - Listeria monocytogenes DPC 3437 7(+++) - Listeria monocytogenes Scott A A(+++) -
NOTE: (Diameter of the zone: Zones of size <=9 mm were designated +; of 10-15 mm were designated ++; of 16-21 mm were designated +++; of >= 22 mm were designated ++++). -
NOTE: not active against Bacillus coagulans LMG 6326 NOTE: not active against Bacillus coagulans LMG 6326T - Bacillus subtilis LMG 8198 - Bacillus subtilis DPC 3344 - Bacillus thuringiensis IBS 14 - Bacteroides fragilis LMG 10263 - Bifidobacterium adolescensis DPC 6169 - Bifidobacterium animalis DPC 5420 - Bifidobacterium breve DPC 6166 - Bifidobacterium breve BB12 - Bifidobacterium longum DSMZ 20097 - Bifidobacterium merycicum DSMZ 6493 - Bifidobacterium pseudolongum DSMZ 20092 - Campylobacter jejuni CI 120 - Clostridium sporogenes NCIMB 9584 - Enterobacter sakazaki ATCC 12869 - Enterobacter sakazaki NCTC 8155 - Enterococcus casseliflavus LMG 10745T - Enterococcus durans LMG 10746T - Enterococcus faecalis LMG 7973T - Enterococcus faecium LMG 7973T - Enterococcus hirae LMG 6399T - Eschericia coli 3786 - Lactobacillus acidopuilus NCDO 1697 - Lactobacillus bulgaricis LMG 6901t - Lactobacillus casei ATCC 334 - Lactobacillus curvatus LMG 12009 - Lactobacillus gallinarum LMG 9435T - Lactobacillus helveticus LH1 - Lactobacillus paracasei 338 - Lactobacillus reuteri NCIMB 11951 - Lactobacillus rhamnosus GG - Lactobacillus ruminis DSM 20403T - Lactobacillus salivarius UCC 118 - Lactococcus lactis HP - Leuconostoc DPC 139 - Leuconostoc DPC 240 - Micrococcus luteus DPC 6275 - Micrococcus luteus LMG 3293 - Pediococcus pentasaceus LMG 11488 - Propionibacterium avidium LMG - Proprionibacterium acne LMG - Propionibacterium jensenii NCFB 565 - Propionibacterium jensenii NCFB 850T - Pseudomonas putida ATCC 33015 - Pseudomonas putida ATCC 17522 - Salmonella enterica serovar Typhimurium DPC 6046 - Salmonella enterica serovar Derby DPC 6049 - Staphylococcus aureus ATCC 25923 - Staphylococcus aureus DPC 3767 - Staphylococcus saphrophyticus DPC 6289 - Streptococcus algalactiae LMG 14694t - Streptococcus bovis DPC 5244 - Streptococcus dysgalactia DPC 5345 - Steptococcus mutans 6159 DPC 6159 - Steptococcus mutans 6155 DPC 6155 - Steptococcus mutans 6143 DPC 6143.}
Thuricin CD is a two-component antimicrobial peptide system with sulfur to α-carbon linkages. NMR analysis of Trn-α and Trn-β sheds light on the nature of the posttranslational modifications at residues 21, 25, and 28.
UniProt Entry: C2TQ80
PDB Entry2L9X resolved by NMR
PubMed=21526839;DOI=10.1021/ja201802f
Sit C.S., McKay R.T., Hill C., Ross R.P., Vederas J.C.
"The 3D structure of thuricin CD, a two-component bacteriocin with cysteine sulfur to ?-carbon cross-links.", J. Am. Chem. Soc. 133:7680-7683(2011).
PubMed=22645259;DOI=10.1101/gr.134437.111
Zwick M.E., Joseph S.J., Didelot X., Chen P.E., Bishop-Lilly K.A., Stewart A.C., Willner K., Nolan N., Lentz S., Thomason M.K., Sozhamannan S., Mateczun A.J., Du L., Read T.D.
"Genomic characterization of the Bacillus cereus sensu lato species: Backdrop to the evolution of Bacillus anthracis.", Genome Res. 22:1512-1524(2012).
Gene id | Name | Description | Location |
BACGene964 | ABC transporter, permease protein similar to ABC-type transport system involved in multi-copper enzyme maturation,permease component COG1277; similar to Putative ABC transporter, permeaseprotein of Clostridium difficile UniRef RepID=Q187Z2_CLOD6 | 49..564 | |
BACGene965 | hypothetical protein | 609..758 | |
BACGene966 | hypothetical protein | 806..949 | |
BACGene967 | Radical SAM domain protein similar to Arylsulfatase regulator (Fe-S oxidoreductase) COG0641; similar toRadical SAM domain protein of Petrotoga mobilis SJ95 UniRef RepID=A9BJJ9_PETMO | 1081..2580 | |
BACGene968 | Radical SAM domain protein similar to Arylsulfatase regulator (Fe-S oxidoreductase) COG0641; similar toRadical SAM domain protein of Thermosinus carboxydivorans Nor1 UniRefRepID=A1HP80_9FIRM | 2595..3821 | |
BACGene969 | Carboxyl-terminal protease similar to Carboxyl-terminal protease of Thermotogaceae UniRefRepID=A8F4J5_THELT | 4020..5045 | |
BACGene970 | Recombinase similar to Recombinase of Bacillaceae UniRef RepID=A7GL27_BACCN | complement(5669..6040) | |
BACGene971 | IS3-family transposase, OrfB similar to IS3-family transposase, OrfB of root UniRef RepID=A3I710_9BACI | 6143..6289 | |
BACGene972 | hypothetical protein similar to Putative uncharacterized protein of Bacillus cereus G9241 UniRefRepID=Q4MUZ2_BACCE | complement(6316..6765) | |
BACGene973 | hypothetical protein similar to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase/S1 RNA-bindingdomain protein PRK00087; similar to Possible ribosomal protein S1 of Bacilluscereus UniRef RepID=Q4V1L4_BACCZ | 6983..7894 | |
BACGene974 | hypothetical protein similar to Putative uncharacterized protein of Bacillus cereus UniRefRepID=Q4MUZ4_BACCE | 7894..8865 | |
BACGene975 | hypothetical protein | 9203..9535 | |
BACGene976 | hypothetical protein similar to Putative uncharacterized protein of Listeria monocytogenes str. 1/2aF6854 UniRef RepID=Q4ESH9_LISMO | 9601..10020 | |
BACGene977 | DEAD/DEAH box helicase domain protein similar to Superfamily II helicase COG1204; similar to DEAD/DEAH box helicasedomain protein of Listeria monocytogenes str. 1/2a F6854 UniRefRepID=Q4ESI0_LISMO | 10124..13372 | |
BACGene978 | hypothetical protein similar to Putative uncharacterized protein of Desulfitobacterium hafniense Y51UniRef RepID=Q24ZE7_DESHY | complement(13529..14044) | |
BACGene979 | hypothetical protein | complement(14068..14370) | |
BACGene980 | hypothetical protein | complement(14446..15459) |
........10 ........20 ........30 ........40 | | | | GNAACVIGCI GSCVISEGIG SLVGTAFTLG |
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔30 | 30 | Lantibiotic Thuricin CD Trn-α.
Feature identifier = |
Modified residue | 21 | dehydro-Ser. | |
Modified residue | 25 | dehydro-Thr. | |
Modified residue | 28 | dehydro-Thr. | |
Cross-link | 5↔28 | 24 | α-carbon thioether (Cys-Ser). |
Cross-link | 9↔25 | 17 | α-carbon thioether (Cys-Thr). |
Cross-link | 13↔21 | 9 | α-carbon thioether (Cys-Thr). |
HELIX | 3↔9 | 7 | |
HELIX | 12↔16 | 5 | |
HELIX | 21↔24 | 4 |
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | DHKMPQRWY |
Common amino acids | G |
Mass (Da) | 2763 |
Net charge | -1 |
Isoelectric point | 3.85 |
Basic residues | 0 |
Acidic residues | 1 |
Hydrophobic residues | 13 |
Polar residues | 16 |
Aliphatic residues | 9 |
Tiny residues | 13 |
Boman Index | 31.68 |
Hydropathy Index | 1.343 |
Aliphatic Index | 117 |
Instability Index | 25.25 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 125 M-1 cm-1 |
Absorbance 280nm | 4.31 |
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