Bacteriocin: Enterocin W beta

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Bacteriocin: Enterocin W beta

Accession: BAC214

Classification
Class: Class IIb
Genetics
Gene: enwB
Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsBacillus coagulans JCM 2257 (MIC=9.7µM) - Bacillus circulans JCM 2504 (MIC=77.9µM) - Listeria innocua ATCC 33090 (MIC=311.6µM) - Pediococcus pentosaceus JCM 5885 (MIC=77.9µM) - Enterococcus faecalis JCM 5803 (MIC=19.7µM) - Lactococcus lactis subsp. lactis JCM 7638 (MIC=4.8µM) - Lactococcus lactis subsp. lactis ATCC 19435 (MIC=36.7µM) - Lactobacillus sakeisubsp. sakei JCM 1157 (MIC=77µM) -

NOTE: not active against Bacillus subtilis JCM 146 NOTE: not active against Bacillus subtilis JCM 1465 - Kocuria rhizophila NBRC 12708 (>3.2 μM)}

NUCLEOTIDE SEQUENCE, STRAIN=NKR-4-1.
PubMed=22138996;DOI=10.1128/AEM.06497-11
Sawa N., Wilaipun P., Kinoshita S., Zendo T., Leelawatcharamas V., Nakayama J., Sonomoto K.
"Isolation and Characterization of Enterocin W, a Novel Two-Peptide Lantibiotic Produced by Enterococcus faecalis NKR-4-1.", Appl. Environ. Microbiol. 78:900-903(2012).
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End Note
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Gene structure
genome of Enterocin W beta

Gene idNameDescriptionLocation
BACGene955enwAenterocin W alfa enw of two-peptide lantibiotic55..237
BACGene956enwBenterocin W beta enw of two-peptide lantibiotic256..441
enwAenwB
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 VTTSIPCTVM VSAAVCPTLV CSNKCGGRG

Wheel representation
wheel representation of Enterocin W beta
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids DEFHQWY
Common amino acids V
Mass (Da) 2
Net charge +2
Isoelectric point 8.34
Basic residues 2
Acidic residues 0
Hydrophobic residues 9
Polar residues 15
Aliphatic residues 7
Tiny residues 8
Boman Index -3.64
Hydropathy Index 0.803
Aliphatic Index 83.79
Instability Index 30.51 (stable)
Half Life Mammalian : 100 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 250 M-1 cm-1
Absorbance 280nm 8.93

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