Bacteriocin: LCI
Accession: BAC212
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Bacillus subtilis A14 [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesBacillaceaeBacillus
Target organismsXanthonionas campestris pv. oryzae G - Xanthonionas campestris pv. oryzae X4 - Xanthonionas campestris pv. oryzae X17 - Xanthonionas campestris pv. oryzae X61 - Pseudomonas solanacearum -
NOTE: not active against Erwinia carotovora subsp. carotovora - Escherichia coli
Description
FUNCTION: May bind DNA or mRNA.
SUBCELLULAR LOCATION: Secreted.
MISCELLANEOUS:
- Heat stable.
- Resistant to proteolysis by tryspin and pepsin, but susceptible to pronase E and proteinase K.
SUBCELLULAR LOCATION: Secreted.
MISCELLANEOUS:
- Heat stable.
- Resistant to proteolysis by tryspin and pepsin, but susceptible to pronase E and proteinase K.
Uniprot and PDB links
UniProt Entry: P82243
PDB Entry2B9K resolved by NMR
[Ref. 1]
PROTEIN SEQUENCE OF 1-27, FUNCTION, AND STABILITY, STRAIN=A014.
Liu J.-Y., Pan N.-S., Chen Z.-L.
"Characterization of an anti-rice bacterial blight polypeptide LCI.", Rice Genet. Newsl. 7:151-154(1990).
Liu J.-Y., Pan N.-S., Chen Z.-L.
"Characterization of an anti-rice bacterial blight polypeptide LCI.", Rice Genet. Newsl. 7:151-154(1990).
PROTEIN STRUCTURE, STRAIN=A014.
PubMed=21449609;DOI=10.1021/bi200123w
Xia, B.; Gong, W.; Lu, G.
"Solution Structure of LCI, a Novel Antimicrobial Peptide from Bacillus subtilis", Biochemistry, 50 (18): 3621–3627 (2011).
PubMed=21449609;DOI=10.1021/bi200123w
Xia, B.; Gong, W.; Lu, G.
"Solution Structure of LCI, a Novel Antimicrobial Peptide from Bacillus subtilis", Biochemistry, 50 (18): 3621–3627 (2011).
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Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | AIKLVQSPNG NFAASFVLDG TKWIFKSKYY DSSKGYWVGI YEVWDRK |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔47 | 47 | Antimicrobial peptide LCI. Feature identifier = PRO_0000064937. |
| STRAND | 3↔7 | 5 | |
| STRAND | 15↔18 | 4 | |
| STRAND | 21↔30 | 10 | |
| TURN | 32↔34 | 3 | |
| STRAND | 35↔43 | 9 |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | CHM |
| Common amino acids | K |
| Mass (Da) | 5 |
| Net charge | +3 |
| Isoelectric point | 9.85 |
| Basic residues | 7 |
| Acidic residues | 4 |
| Hydrophobic residues | 18 |
| Polar residues | 16 |
| Aliphatic residues | 9 |
| Tiny residues | 12 |
| Boman Index | -54.26 |
| Hydropathy Index | -0.351 |
| Aliphatic Index | 72.55 |
| Instability Index | 13.08 (stable) |
| Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 22460 M-1 cm-1 |
| Absorbance 280nm | 488.26 |
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