Bacteriocin: Laterosporulin

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Bacteriocin: Laterosporulin

Accession: BAC211

Classification
Class: IId
Genetics
Gene: laterosporulin
Producer and target organisms

Producer Organism: Brevibacillus sp. GI-9 [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesPaenibacillaceaeBrevibacillus

Target organismsBacillus subtilis MTCC 121 (MIC=1.5µg/ml) - Staphylococcus aureus MTCC 1430 (MIC=1.5µg/ml) - Listeria monocytogenes MTCC 839 (MIC=22.5µg/ml) - Pseudomonas aeruginosa MTCC 1934 (MIC=15µg/ml) - Escherichia coli MTCC 1610 (MIC=7.5µg/ml) - Vibrio cholerae (MIC=15µg/ml) -

NOT active against: tested yeast or fungi

Description
Physicochemical features:
- incubation of laterosporulin with proteolytic enzymes (pepsin, trypsin, chymotrypsin, proteinase K, pronase E) did not show any reduction in its antimicrobial activity.

- fully heat stable as it keeps its antimicrobial activity after exposure to 120C for 15 min.
Uniprot and PDB links

UniProt Entry: H1ZZ98

PDB Entry4OZK resolved by X-RAY

NUCLEOTIDE SEQUENCE, STRAIN=GI-9.
PubMed=22403615;DOI=10.1371/journal.pone.0031498
Singh P.K., Ashish C., Sharma V., Patil P.B., Korpole S.
"Identification, purification and characterization of laterosporulin, a novel bacteriocin produced by Brevibacillus sp. strain GI-9.", PLoS ONE 7:e31498-e31498(2012).
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End Note
Reference Manager
BibTeX
Gene structure
genome of Laterosporulin

Gene idNameDescriptionLocation
BACGene948transcriptional regulator ORF135..727
BACGene949laterosporulinputative bacteriocin982..1134
BACGene950laterosporulinhypothetical protein ORF21223..1708
BACGene951laterosporulinABC transporter ORF31711..3561
BACGene952laterosporulinputative dehydrogenase ORF43566..4000
laterosporulinlaterosporulinlaterosporulinlaterosporulin
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 ACQCPDAISG WTHTDYQCHG LENKMYRHVY AICMNGTQVY CRTEWGSSC

Wheel representation
wheel representation of Laterosporulin
3D Structure
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids F
Common amino acids C
Mass (Da) 5
Net charge +2
Isoelectric point 6.73
Basic residues 6
Acidic residues 4
Hydrophobic residues 10
Polar residues 23
Aliphatic residues 5
Tiny residues 10
Boman Index -82.3
Hydropathy Index -0.49
Aliphatic Index 41.84
Instability Index 45.65 (unstable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 17335 M-1 cm-1
Absorbance 280nm 361.15

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