Bacteriocin: Laterosporulin
Accession: BAC211
Classification
Class: IId
Genetics
Gene: laterosporulin
Producer and target organisms
Producer Organism: Brevibacillus sp. GI-9 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacillales
Paenibacillaceae
Brevibacillus
Target organismsBacillus subtilis MTCC 121 (MIC=1.5µg/ml) - Staphylococcus aureus MTCC 1430 (MIC=1.5µg/ml) - Listeria monocytogenes MTCC 839 (MIC=22.5µg/ml) - Pseudomonas aeruginosa MTCC 1934 (MIC=15µg/ml) - Escherichia coli MTCC 1610 (MIC=7.5µg/ml) - Vibrio cholerae (MIC=15µg/ml) -
NOT active against: tested yeast or fungi
Description
Physicochemical features:
- incubation of laterosporulin with proteolytic enzymes (pepsin, trypsin, chymotrypsin, proteinase K, pronase E) did not show any reduction in its antimicrobial activity.
- fully heat stable as it keeps its antimicrobial activity after exposure to 120°C for 15 min.
- incubation of laterosporulin with proteolytic enzymes (pepsin, trypsin, chymotrypsin, proteinase K, pronase E) did not show any reduction in its antimicrobial activity.
- fully heat stable as it keeps its antimicrobial activity after exposure to 120°C for 15 min.
Uniprot and PDB links
UniProt Entry: H1ZZ98
PDB Entry4OZK resolved by X-RAY
NUCLEOTIDE SEQUENCE, STRAIN=GI-9.
PubMed=22403615;DOI=10.1371/journal.pone.0031498
Singh P.K., Ashish C., Sharma V., Patil P.B., Korpole S.
"Identification, purification and characterization of laterosporulin, a novel bacteriocin produced by Brevibacillus sp. strain GI-9.", PLoS ONE 7:e31498-e31498(2012).
PubMed=22403615;DOI=10.1371/journal.pone.0031498
Singh P.K., Ashish C., Sharma V., Patil P.B., Korpole S.
"Identification, purification and characterization of laterosporulin, a novel bacteriocin produced by Brevibacillus sp. strain GI-9.", PLoS ONE 7:e31498-e31498(2012).
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Gene structure
Gene id | Name | Description | Location |
BACGene948 | transcriptional regulator ORF1 | 35..727 | |
BACGene949 | laterosporulin | putative bacteriocin | 982..1134 |
BACGene950 | laterosporulin | hypothetical protein ORF2 | 1223..1708 |
BACGene951 | laterosporulin | ABC transporter ORF3 | 1711..3561 |
BACGene952 | laterosporulin | putative dehydrogenase ORF4 | 3566..4000 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | ACQCPDAISG WTHTDYQCHG LENKMYRHVY AICMNGTQVY CRTEWGSSC |
Wheel representation
3D Structure
Composition
Hydrophobicity
Composition
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | F |
Common amino acids | C |
Mass (Da) | 5 |
Net charge | +2 |
Isoelectric point | 6.73 |
Basic residues | 6 |
Acidic residues | 4 |
Hydrophobic residues | 10 |
Polar residues | 23 |
Aliphatic residues | 5 |
Tiny residues | 10 |
Boman Index | -82.3 |
Hydropathy Index | -0.49 |
Aliphatic Index | 41.84 |
Instability Index | 45.65 (unstable) |
Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 17335 M-1 cm-1 |
Absorbance 280nm | 361.15 |
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