Bacteriocin: Enterocin EJ97

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Bacteriocin: Enterocin EJ97

Accession: BAC203

Classification
Class: Unclassified
Genetics
Gene: ej97a
Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsBacillus circulans (12µg/ml) - Bacillus coagulans CECT 12 (2µg/ml) - Bacillus megaterium (4µg/ml) - Bacillus stearothermophilus CECT 49 (14µg/ml) - Bacillus stearothermophilus CECT 148 (12µg/ml) - Bacillus subtilis (12µg/ml) - Paenibacillus macerans CECT 19 (4µg/ml) - Enterococcus faecalis ssp. faecalis S-13 (6µg/ml) - Enterococcus faecalis ssp. faecalis S-14 (8µg/ml) - Enterococcus faecalis ssp. faecalis S-32 (12µg/ml) - Enterococcus faecalis ssp. faecalis S-86 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-39 (6µg/ml) - Enterococcus faecalis ssp. liquefaciens S-40 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-47 (12µg/ml) - Enterococcus faecalis ssp. liquefaciens S-48 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-54 (6µg/ml) - Enterococcus faecalis ssp. liquefaciens S-55 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-71 (12µg/ml) - Enterococcus faecalis ssp. zymogenes S-50 (6µg/ml) - Enterococcus faecalis ssp. zymogenes S-79 (8µg/ml) - Enterococcus faecalis ssp. zymogenes S-81 (12µg/ml) - Enterococcus faecalis JH2-2 (6µg/ml) - Enterococcus faecalis OG1X (6µg/ml) - Enterococcus faecium S-29 (8µg/ml) - Enterococcus faecium S-60 (6µg/ml) - Listeria grayi CECT 931 (16µg/ml) - Listeria innocua CECT 4030 (16µg/ml) - Listeria ivanovii CECT 913 (8µg/ml) - Listeria monocytogenes CECT 4032 (14µg/ml) - Listeria murrayi CECT 942 (22µg/ml) - Listeria welshimeri CECT 919 (40µg/ml) - Staphylococcus aureus (16µg/ml)

NUCLEOTIDE SEQUENCE.
MEDLINE=22507691;PubMed=12620853;DOI=10.1128/AEM.69.3.1633-1641.2003
Sanchez-Hidalgo M., Maqueda M., Galvez A., Abriousel H., Valdivia E., Martinez-Bueno M.
"The genes coding for enterocin EJ97 production by Enterococcus faecalis EJ97 are located on a conjugative plasmid.", Appl. Environ. Microbiol. 69:1633-1641(2003).
PURIFICATION AND ACTIVITY.
PubMed=9871020;DOI=10.1007/s002030050678
Galvez A., Valdivia E., Abriouel H., Camafeita E., Mendez E., Martinez-Bueno M., Maqueda M.
"Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97", Arch Microbiol. 171:5965(1998).
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End Note
Reference Manager
BibTeX
Gene structure
genome of Enterocin EJ97

Gene idNameDescriptionLocation
BACGene918tnptransposase510..1199
BACGene919tnphypothetical protein ORFAcomplement(1293..1445)
BACGene920ej97aEJ97 enterocin bacteriocin1581..1715
BACGene921ej97bEj97B protein ABC transporter for enterocin EJ971796..3547
BACGene922ej97bhypothetical protein ORFBcomplement(3576..3788)
BACGene923ej97cEj97C protein3604..3804
BACGene924ej97chypothetical protein ORFCcomplement(3817..3933)
BACGene925ej97dEj97D protein4098..4292
BACGene926res97Res97 protein resolvase4452..5093
BACGene927res97hypothetical protein ORF75145..5351
BACGene928res97hypothetical protein ORFDcomplement(5335..5550)
BACGene929repBRepB protein copy control and partitioning5492..6238
BACGene930repBhypothetical protein ORF96241..6537
BACGene931repAreplication-associated protein A replication6874..7857
BACGene932traCTraC protein Pheromone binding protein7877..9514
BACGene933traBTraB protein shutdown of endogenous pheromone production9525..10679
BACGene934traATraA protein negative regulatorcomplement(10741..11334)
tnptnpej97aej97bej97bej97cej97cej97dres97res97res97repBrepBrepAtraCtraBtraA
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 MLAKIKAMIK KFPNPYTLAA KLTTYEINWY KQQYGRYPWE RPVA

Wheel representation
wheel representation of Enterocin EJ97
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids CDHS
Common amino acids K
Mass (Da) 5
Net charge +6
Isoelectric point 10.39
Basic residues 8
Acidic residues 2
Hydrophobic residues 15
Polar residues 11
Aliphatic residues 7
Tiny residues 6
Boman Index -53.64
Hydropathy Index -0.589
Aliphatic Index 71.14
Instability Index 19.46 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 18450 M-1 cm-1
Absorbance 280nm 429.07

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