Bacteriocin: Enterocin EJ97
Accession: BAC203
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Enterococcaceae
Enterococcus
Target organismsBacillus circulans (12µg/ml) - Bacillus coagulans CECT 12 (2µg/ml) - Bacillus megaterium (4µg/ml) - Bacillus stearothermophilus CECT 49 (14µg/ml) - Bacillus stearothermophilus CECT 148 (12µg/ml) - Bacillus subtilis (12µg/ml) - Paenibacillus macerans CECT 19 (4µg/ml) - Enterococcus faecalis ssp. faecalis S-13 (6µg/ml) - Enterococcus faecalis ssp. faecalis S-14 (8µg/ml) - Enterococcus faecalis ssp. faecalis S-32 (12µg/ml) - Enterococcus faecalis ssp. faecalis S-86 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-39 (6µg/ml) - Enterococcus faecalis ssp. liquefaciens S-40 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-47 (12µg/ml) - Enterococcus faecalis ssp. liquefaciens S-48 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-54 (6µg/ml) - Enterococcus faecalis ssp. liquefaciens S-55 (8µg/ml) - Enterococcus faecalis ssp. liquefaciens S-71 (12µg/ml) - Enterococcus faecalis ssp. zymogenes S-50 (6µg/ml) - Enterococcus faecalis ssp. zymogenes S-79 (8µg/ml) - Enterococcus faecalis ssp. zymogenes S-81 (12µg/ml) - Enterococcus faecalis JH2-2 (6µg/ml) - Enterococcus faecalis OG1X (6µg/ml) - Enterococcus faecium S-29 (8µg/ml) - Enterococcus faecium S-60 (6µg/ml) - Listeria grayi CECT 931 (16µg/ml) - Listeria innocua CECT 4030 (16µg/ml) - Listeria ivanovii CECT 913 (8µg/ml) - Listeria monocytogenes CECT 4032 (14µg/ml) - Listeria murrayi CECT 942 (22µg/ml) - Listeria welshimeri CECT 919 (40µg/ml) - Staphylococcus aureus (16µg/ml)
UniProt Entry: Q8KMU4
PDB EntryUnknown
MEDLINE=22507691;PubMed=12620853;DOI=10.1128/AEM.69.3.1633-1641.2003
Sanchez-Hidalgo M., Maqueda M., Galvez A., Abriousel H., Valdivia E., Martinez-Bueno M.
"The genes coding for enterocin EJ97 production by Enterococcus faecalis EJ97 are located on a conjugative plasmid.", Appl. Environ. Microbiol. 69:1633-1641(2003).
PubMed=9871020;DOI=10.1007/s002030050678
Galvez A., Valdivia E., Abriouel H., Camafeita E., Mendez E., Martinez-Bueno M., Maqueda M.
"Isolation and characterization of enterocin EJ97, a bacteriocin produced by Enterococcus faecalis EJ97", Arch Microbiol. 171:59–65(1998).
PubMed=20385607;DOI=10.1093/protein/gzq020
Neira J.L., Contreras L.M., de los Panos O.R., Sanchez-Hidalgo M., Martinez-Bueno M., Maqueda M, Rico M.
"Structural characterisation of the natively unfolded enterocin EJ97", Protein Eng Des Sel. 23(7):507-18(2010).
Gene id | Name | Description | Location |
BACGene918 | tnp | transposase | 510..1199 |
BACGene919 | tnp | hypothetical protein ORFA | complement(1293..1445) |
BACGene920 | ej97a | EJ97 enterocin bacteriocin | 1581..1715 |
BACGene921 | ej97b | Ej97B protein ABC transporter for enterocin EJ97 | 1796..3547 |
BACGene922 | ej97b | hypothetical protein ORFB | complement(3576..3788) |
BACGene923 | ej97c | Ej97C protein | 3604..3804 |
BACGene924 | ej97c | hypothetical protein ORFC | complement(3817..3933) |
BACGene925 | ej97d | Ej97D protein | 4098..4292 |
BACGene926 | res97 | Res97 protein resolvase | 4452..5093 |
BACGene927 | res97 | hypothetical protein ORF7 | 5145..5351 |
BACGene928 | res97 | hypothetical protein ORFD | complement(5335..5550) |
BACGene929 | repB | RepB protein copy control and partitioning | 5492..6238 |
BACGene930 | repB | hypothetical protein ORF9 | 6241..6537 |
BACGene931 | repA | replication-associated protein A replication | 6874..7857 |
BACGene932 | traC | TraC protein Pheromone binding protein | 7877..9514 |
BACGene933 | traB | TraB protein shutdown of endogenous pheromone production | 9525..10679 |
BACGene934 | traA | TraA protein negative regulator | complement(10741..11334) |
........10 ........20 ........30 ........40 ........50 | | | | | MLAKIKAMIK KFPNPYTLAA KLTTYEINWY KQQYGRYPWE RPVA |
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | CDHS |
Common amino acids | K |
Mass (Da) | 5 |
Net charge | +6 |
Isoelectric point | 10.39 |
Basic residues | 8 |
Acidic residues | 2 |
Hydrophobic residues | 15 |
Polar residues | 11 |
Aliphatic residues | 7 |
Tiny residues | 6 |
Boman Index | -53.64 |
Hydropathy Index | -0.589 |
Aliphatic Index | 71.14 |
Instability Index | 19.46 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 18450 M-1 cm-1 |
Absorbance 280nm | 429.07 |
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