Bacteriocin: Thurincin H
Accession: BAC201
Producer Organism: Bacillus thuringiensis SF361 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacillales
Bacillaceae
Bacillus
Bacillus cereus group
Target organismsActive [− (no activity) to ++++ (highest activity)] against : Bacillus subtilis ATCC 6633 (++) - Bacillus subtilis ATCC 6537 (+) - Bacillus subtilis CU1065 5(WT) (+++) - Bacillus subtilis LRB90 (+) - Bacillus subtilis LRB91 (+) - Bacillus cereus F4552 (+++++) - Bacillus cereus F4810 (+++++) - Bacillus cereus Northl - ) - Bacillus cereus Northview P2E018 (+++++) - Geobacillus stearothermophilus ATCC 12980 (+++++) - Bacillus thuringiensis EG10368 (+++++) - Bacillus megaterium LRB89 (+++++) - Listeria monocytogenes F2 586 1053 (++++) - Listeria monocytogenes 2289 (++++) - Listeria innocua ATCC 2283 (+++++) - Listeria ivanovii ATCC 19119 (+++++) - Micrococcus luteus (+) - Staphylococcus aureus ATCC 9144 (+) - Staphylococcus aureus ATCC 8095 (+) - Carnobacterium piscicola CU216 (+++++) -
NOT active against: Bacillus cereus ATCC 11778 - Paenibacillus kobensis M - Staphylococcus aureus ATCC 25923 - Lactobacillus acidophilus L-39 - Lactobacillus plantarum B246 - Lactobacillus plantarum EH22G - Lactobacillus helveticus L-31 - Lactobacillus delbrueckii 4797 - Lactobacillus buchneri ATCC 4005 - Lactobacillus fermentum ATCC14931 - Lactobacillus casei 30SC - Lactobacillus brevis B155 - Leuconostoc mesenteroides C-33 - Leuconostoc mesenteroides 548D - Enterococcus faecalis 8043 - Pediococcus cerevisiae E66 - Escherichia coli BF2 - Escherichia coli O157:H7 ATCC 43889 - Salmonella Typhimurium ATCC 14028 - Salmonella Typhimurium Montevideo - Salmonella Enteritidis
Thurincin H is post-translationally modified, with four S-Cα thioether crosslinks (Ref 3).
UniProt Entry: B5U2V4
PDB Entry2LBZ resolved by NMR
PubMed=19083012;DOI=10.1007/s00425-008-0870-6
Lee K.D., Gray E.J., Mabood F., Jung W.J., Charles T., Clark S.R., Ly A., Souleimanov A., Zhou X., Smith D.L.
"The class IId bacteriocin thuricin-17 increases plant growth.", Planta 229:747-755(2009).
PubMed=19732155;DOI=10.1111/j.1574-6968.2009.01749.x
Lee H., Churey J.J., Worobo R.W.
"Biosynthesis and transcriptional analysis of thurincin H, a tandem repeated bacteriocin genetic locus, produced by Bacillus thuringiensis SF361.", FEMS Microbiol. Lett. 299:205-213(2009).
PubMed=21786372;DOI=10.1002/anie.201102527
Sit C.S., van Belkum M.J., McKay R.T., Worobo R.W., Vederas J.C.
"The 3 D Solution Structure of Thurincin H, a Bacteriocin with Four Sulfur to α-Carbon Crosslinks.", Angew Chem Int Ed Engl. (2011).
Gene id | Name | Description | Location |
BACGene903 | thnP | ThnP putative leader peptide-processing serine protease | complement(1..1398) |
BACGene904 | thnE | ThnE ABC transporter; putative permease protein | complement(1619..2266) |
BACGene905 | thnD | ThnD ABC transporter; putative ATP-binding protein | complement(2256..3122) |
BACGene906 | thnR | ThnR GntR family transcriptional regulator; putative response regulator | complement(3119..3502) |
BACGene907 | thnA1 | thurincin H precursor bacteriocin precursor; thurincin H1 | 3822..3944 |
BACGene908 | thnA2 | thurincin H precursor bacteriocin precursor; thurincin H2 | 4051..4173 |
BACGene909 | thnA3 | thurincin H precursor bacteriocin precursor; thurincin H3 | 4279..4401 |
BACGene910 | thnB | ThnB Fe-S oxidoreductase; radical SAM domain protein; putative bacteriocin processingprotein | 4566..5945 |
BACGene911 | thnT | ThnT ABC transporter; putative ABC-family secretion protein | 6110..7819 |
BACGene912 | thnI | ThnI putative immunity protein | 7854..8141 |
........10 ........20 ........30 ........40 | | | | DWTCWSCLVC AACSVELLNL VTAATGASTA S |
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | FHIKMPQRY |
Common amino acids | A |
Mass (Da) | 3139.51 |
Net charge | -2 |
Isoelectric point | 3.55 |
Basic residues | 0 |
Acidic residues | 2 |
Hydrophobic residues | 15 |
Polar residues | 14 |
Aliphatic residues | 7 |
Tiny residues | 11 |
Boman Index | 7.33 |
Hydropathy Index | 0.965 |
Aliphatic Index | 97.74 |
Instability Index | 35.15 (stable) |
Half Life |
Mammalian : 1.1 hour Yeast : 3 min E. coli : >10 hour |
Extinction Coefficient | 11250 M-1 cm-1 |
Absorbance 280nm | 375 |
Users comments for Thurincin H
2013-02-09 07:54:19
Producer strain is now specified: Bacillus thuringiensis SF361 thanks for your comment
2013-02-08 13:22:33
DOES ANYONE KNOWS WHICH STRAINS OF BT PRODUCE THURINCIN H ? THANKS