Bacteriocin: Zoocin A

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Bacteriocin: Zoocin A

Accession: BAC198

Classification
Class: Unclassified
Genetics
Gene: zooA
Producer and target organisms

Producer Organism: Streptococcus equi subsp. zooepidemicus [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsGram-positive: Streptococcus pyogenes (4/4) - Streptococcus dysgalactiae subsp. equisimilis (1/1) -

NOTE:not active against Micrococcus luteus - Streptococcus anginosus - Streptococcus uberis - Lactococcus lactis subsp. lactis

Description
SEQUENCE: zoocin A is translated as a 285-aa precursor protein and released into the environment by proteolytic cleavage of a 23-aa leader sequence to yield the functional protein of 262 aa.

MODE OF ACTION:
Zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
Uniprot and PDB links

UniProt Entry: O54308

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=4881.
MEDLINE=97311418;PubMed=9168135;DOI=10.1016/S0378-1119(96)00859-1
Simmonds R.S., Simpson W.J., Tagg J.R.
"Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.", Gene 189:255-261(1997).
NUCLEOTIDE SEQUENCE, STRAIN=4881.
Gargis A.S., O'Rourke A.-L.D., Sloan G.L., Simmonds R.S.
"", Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=9g.
PubMed=19357799;DOI=10.1007/s00239-009-9221-x
Gargis A.S., O'Rourke A.L., Sloan G.L., Simmonds R.S.
"Prevalence and acquisition of the genes for zoocin A and zoocin A resistance in Streptococcus equi subsp. zooepidemicus.", J. Mol. Evol. 68:498-505(2009).
FUNCTION, MODE OF ACTION
PubMed=15251198;DOI=10.1016/j.femsle.2004.05.038
Heath L.S., Heath H.E., LeBlanc P.A., Smithberg S.R., Dufour M., Simmonds R.S., Sloan G.L.
"The streptococcolytic enzyme zoocin A is a penicillin-binding protein", FEMS Microbiol Lett. 15;236(2):205-11 (2004).
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End Note
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BibTeX
Gene structure
genome of Zoocin A

Gene idNameDescriptionLocation
BACGene888rggRgg putative transcriptional regulator10662..10940
BACGene889tnpATnpA putative IS200-like transposasecomplement(11160..11624)
BACGene890zifzoocin A immunity factor encodes producer cell immunity for the endopeptidase zoocin Acomplement(11954..13186)
BACGene891zooAzoocin A endopeptidase endopeptidase; bacteriocin-like inhibitory substance; similar to lysostaphin13433..14290
BACGene892tnpBTnpB putative transposasecomplement(14377..15636)
BACGene893tnpBTnpB putative IS204/IS1001/IS1096/IS1165-like transposasecomplement(15758..17017)
BACGene894tnpBputative topology modulation protein FlaR-like protein17334..17708
rggtnpAzifzooAtnpBtnpBtnpB
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 ATYTRPLDTG NITTGFNGYP GHVGVDYAVP VGTPVRAVAN GTVKFAGNGA NHPWMLWMAG NCVLIQHADG MHTGYAHLSK
 ISVSTDSTVK QGQIIGYTGA TGQVTGPHLH FEMLPANPNW QNGFSGRIDP TGYIANAPVF NGTTPTEPTT PTTNLKIYKV
 DDLQKINGIW QVRNNILVPT DFTWVDNGIA ADDVIEVTSN GTRTSDQVLQ KGGYFVINPN NVKSVGTPMK GSGGLSWAQV
 NFTTGGNVWL NTTSKDNLLY GK

Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids
Common amino acids G
Mass (Da) 27998.62
Net charge +7
Isoelectric point 7.79
Basic residues 23
Acidic residues 16
Hydrophobic residues 83
Polar residues 109
Aliphatic residues 52
Tiny residues 60
Boman Index -287.27
Hydropathy Index -0.267
Aliphatic Index 74.35
Instability Index 9.56 (stable)
Half Life Mammalian : 4.4 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 51910 M-1 cm-1
Absorbance 280nm 198.89

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