Bacteriocin: Zoocin A
Accession: BAC198
Classification
Class: Unclassified
Genetics
Gene: zooA
Producer and target organisms
Producer Organism: Streptococcus equi subsp. zooepidemicus [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Streptococcaceae
Streptococcus
Target organismsGram-positive: Streptococcus pyogenes (4/4) - Streptococcus dysgalactiae subsp. equisimilis (1/1) -
NOTE:not active against Micrococcus luteus - Streptococcus anginosus - Streptococcus uberis - Lactococcus lactis subsp. lactis
Description
SEQUENCE: zoocin A is translated as a 285-aa precursor protein and released into the environment by proteolytic cleavage of a 23-aa leader sequence to yield the functional protein of 262 aa.
MODE OF ACTION:
Zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
MODE OF ACTION:
Zoocin A had weak β-lactamase activity, bound radioactive penicillin covalently, and its streptococcolytic activity was inhibited by penicillin. Thus, zoocin A is a penicillin-binding protein and presumably is a D-alanyl endopeptidase.
Uniprot and PDB links
UniProt Entry: O54308
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=4881.
MEDLINE=97311418;PubMed=9168135;DOI=10.1016/S0378-1119(96)00859-1
Simmonds R.S., Simpson W.J., Tagg J.R.
"Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.", Gene 189:255-261(1997).
MEDLINE=97311418;PubMed=9168135;DOI=10.1016/S0378-1119(96)00859-1
Simmonds R.S., Simpson W.J., Tagg J.R.
"Cloning and sequence analysis of zooA, a Streptococcus zooepidemicus gene encoding a bacteriocin-like inhibitory substance having a domain structure similar to that of lysostaphin.", Gene 189:255-261(1997).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=4881.
Gargis A.S., O'Rourke A.-L.D., Sloan G.L., Simmonds R.S.
"", Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
Gargis A.S., O'Rourke A.-L.D., Sloan G.L., Simmonds R.S.
"", Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=9g.
PubMed=19357799;DOI=10.1007/s00239-009-9221-x
Gargis A.S., O'Rourke A.L., Sloan G.L., Simmonds R.S.
"Prevalence and acquisition of the genes for zoocin A and zoocin A resistance in Streptococcus equi subsp. zooepidemicus.", J. Mol. Evol. 68:498-505(2009).
PubMed=19357799;DOI=10.1007/s00239-009-9221-x
Gargis A.S., O'Rourke A.L., Sloan G.L., Simmonds R.S.
"Prevalence and acquisition of the genes for zoocin A and zoocin A resistance in Streptococcus equi subsp. zooepidemicus.", J. Mol. Evol. 68:498-505(2009).
FUNCTION, MODE OF ACTION
PubMed=15251198;DOI=10.1016/j.femsle.2004.05.038
Heath L.S., Heath H.E., LeBlanc P.A., Smithberg S.R., Dufour M., Simmonds R.S., Sloan G.L.
"The streptococcolytic enzyme zoocin A is a penicillin-binding protein", FEMS Microbiol Lett. 15;236(2):205-11 (2004).
PubMed=15251198;DOI=10.1016/j.femsle.2004.05.038
Heath L.S., Heath H.E., LeBlanc P.A., Smithberg S.R., Dufour M., Simmonds R.S., Sloan G.L.
"The streptococcolytic enzyme zoocin A is a penicillin-binding protein", FEMS Microbiol Lett. 15;236(2):205-11 (2004).
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Gene structure
Gene id | Name | Description | Location |
BACGene888 | rgg | Rgg putative transcriptional regulator | 10662..10940 |
BACGene889 | tnpA | TnpA putative IS200-like transposase | complement(11160..11624) |
BACGene890 | zif | zoocin A immunity factor encodes producer cell immunity for the endopeptidase zoocin A | complement(11954..13186) |
BACGene891 | zooA | zoocin A endopeptidase endopeptidase; bacteriocin-like inhibitory substance; similar to lysostaphin | 13433..14290 |
BACGene892 | tnpB | TnpB putative transposase | complement(14377..15636) |
BACGene893 | tnpB | TnpB putative IS204/IS1001/IS1096/IS1165-like transposase | complement(15758..17017) |
BACGene894 | tnpB | putative topology modulation protein FlaR-like protein | 17334..17708 |
Protein sequence
........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | ATYTRPLDTG NITTGFNGYP GHVGVDYAVP VGTPVRAVAN GTVKFAGNGA NHPWMLWMAG NCVLIQHADG MHTGYAHLSK ISVSTDSTVK QGQIIGYTGA TGQVTGPHLH FEMLPANPNW QNGFSGRIDP TGYIANAPVF NGTTPTEPTT PTTNLKIYKV DDLQKINGIW QVRNNILVPT DFTWVDNGIA ADDVIEVTSN GTRTSDQVLQ KGGYFVINPN NVKSVGTPMK GSGGLSWAQV NFTTGGNVWL NTTSKDNLLY GK |
Composition
Hydrophobicity
Composition
Formula | C0
H0
N0
O0
S0 |
Absent amino acids | |
Common amino acids | G |
Mass (Da) | 27998.62 |
Net charge | +7 |
Isoelectric point | 7.79 |
Basic residues | 23 |
Acidic residues | 16 |
Hydrophobic residues | 83 |
Polar residues | 109 |
Aliphatic residues | 52 |
Tiny residues | 60 |
Boman Index | -287.27 |
Hydropathy Index | -0.267 |
Aliphatic Index | 74.35 |
Instability Index | 9.56 (stable) |
Half Life |
Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 51910 M-1 cm-1 |
Absorbance 280nm | 198.89 |
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