Bacteriocin: Carocin D
Accession: BAC193
Classification
Class: Unclassified
Genetics
Gene: caroDK
Producer and target organisms
Producer Organism: Pectobacterium carotovorum subsp. carotovorum [Gram-]
Taxonomy: Bacteria
ProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
Target organismsUnavailable data
Uniprot and PDB links
UniProt Entry: D8WUT9
PDB EntryUnknown
[Ref. 1]
NUCLEOTIDE SEQUENCE, STRAIN=Pcc21.
Roh E., Park T.-H., Lee S., Ryu S., Oh C.-S., Kim D.-H., Park B.-S., Heu S.
"Characterization of a new bacteriocin, carocin D, from Pectobacterium carotovorum subsp. carotovorum.", Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
Roh E., Park T.-H., Lee S., Ryu S., Oh C.-S., Kim D.-H., Park B.-S., Heu S.
"Characterization of a new bacteriocin, carocin D, from Pectobacterium carotovorum subsp. carotovorum.", Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE, STRAIN=Pcc21.
PubMed=20870796;DOI=10.1128/AEM.03103-09
Roh, E., Park, T.H., Kim, M.I., Lee, S., Ryu, S., Oh, C.S., Rhee, S., Kim, D.H., Park, B.S. and Heu, S.
"Characterization of a New Bacteriocin, Carocin D, from Pectobacterium carotovorum subsp. carotovorum Pcc21", Appl. Environ. Microbiol. 76 (22), 7541-7549 (2010).
PubMed=20870796;DOI=10.1128/AEM.03103-09
Roh, E., Park, T.H., Kim, M.I., Lee, S., Ryu, S., Oh, C.S., Rhee, S., Kim, D.H., Park, B.S. and Heu, S.
"Characterization of a New Bacteriocin, Carocin D, from Pectobacterium carotovorum subsp. carotovorum Pcc21", Appl. Environ. Microbiol. 76 (22), 7541-7549 (2010).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene886 | caroDK | carocin D bacteriocin; killer protein | 2005..4515 |
| BACGene887 | caroDI | immunity protein | 4508..4765 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | PNDGDTMTVS GGGGWVSNDD RKGGNDRDNG KGGSAVDFSK NPEKQAIVNP YLAIAIPMPV YPLYGKLGFT INTTAIETEL ANVRAAINTK LATLSAVIGR SLPVVGRVFG VTAAGMWPSS TAPSSLDSIY NQAHQQALAQ LAAQQGVLNK GYNVTAMPAG FVSSLPVSEI KSLPTAPASL LAQSVINTEL SQRQLALTQP TTNAPVANIP VVKAEKTAMP GVYSAKIIAG EPAFQIKVDN TKPALAQNPP KVKDDIQVSS FLSSPVADTH HAFIDFGSDH EPVYVSLSKI VTAEEEKKQV EEAKRREQEW LLRHPITAAE RKLTEIRQVI SFAQQLKESS VATISEKTKT VAVYQEQVNT AAKNRDNFYN QNRGLLSAGI TGGPGYPIYL ALWQTMNNFH QAYFRANNAL EQESHVLNLA RSDLAKAEQL LAENNRLQVE TERTLAEEKE IKRNRVNVST FGTVQTQLSK LLSDFYAVTS LSQSVPSGAL ASFSYNPQGM IGSGKIVGKD VDVLFSIPVK DIPGYKSPIN LDDLAKKNGS LDLPIRLAFS DENGERVLRA FKADSLRIPS SVRGVAGSYD KNTGIFSAEI DGVSSRLVLE NPAFPPTGNV GNTGNTAPDY KALLNTGVDV KPVDKITVTV TPVADPVDID DYIIWLPTAS GSGVEPIYVV FNSNPYGGTE KGKYSKRYYN PDKAGGPILE LDWKNVKIDH AGVDNVKLHT GRFKASVENK VMIERLENIL NGQITATDTD KRFYTHELRE LNRYRNLGIK DGEVPSSIQE ESAVWNDTHT ATLEDYKINE KEQPLYTDAA LQAAYEQELK DALGGKHG |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | C |
| Common amino acids | A |
| Mass (Da) | 89915.84 |
| Net charge | +4 |
| Isoelectric point | 6.28 |
| Basic residues | 98 |
| Acidic residues | 94 |
| Hydrophobic residues | 290 |
| Polar residues | 255 |
| Aliphatic residues | 181 |
| Tiny residues | 200 |
| Boman Index | -1327.78 |
| Hydropathy Index | -0.354 |
| Aliphatic Index | 86.7 |
| Instability Index | 33.39 (stable) |
| Half Life |
Mammalian : >20 hour Yeast : >20 hour E. coli : ? |
| Extinction Coefficient | 80220 M-1 cm-1 |
| Absorbance 280nm | 97 |
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