Bacteriocin: Garvicin ML

Default view

Bacteriocin: Garvicin ML

Accession: BAC192

Classification

Class: ~~Unclassified~~

Genetics

Gene: garML

Producer and target organisms

Producer Organism: Lactococcus garvieae [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsAntimicrobial activity by the agar diffusion test (ADT) & area of the halos of inhibition in mm²: -

Gram-positive : Lactobacillus reuteri 20016 (378) - Lactobacillus helveticus 15009 (492) - Lactobacillus curvatus 2739 (177) - Lactobacillus casei 334 (1 - 174) - Lactobacillus acidophilus 4356 (236) - Lactobacillus sakei 2714 (1 - 445) - Lactococcus lactis BB24 (928) - Lactococcus lactis NZ9000 (163) - Lactococcus lactis DPC5598 (1 - 541) - Lactococcus garvieae 5274 (617) - Lactococcus garvieae 5806 (2 - 807) - Lactococcus garvieae 5807 (2 - 705) - Lactococcus raffinolactis 988 (228) - Pediococcus acidilactici 347 (1 - 398) - Pediococcus pentosaceus FBB61 (1 - 131) - Enterococcus faecium P13 (1 - 291) - Enterococcus faecium L50 (719) - Enterococcus faecialis DBH18 (25) - Enterococcus faecalis P4 (123) - Propionibacterium sp. P6 (190) - Propionibacterium acidipropionici 563 (864) - Clostridium tyrobutiricum 3.5CT (651) - Clostridium tyrobutiricum 1754 (839) - Clostridium perfringens 376 (352) - Clostridium botulinum 551 (574) - Listeria monocytogenes 4032 (662) - Listeria ivanovii 913 (1 - 020) - Listeria seeligeri 917 (684) - Listeria grayi 931 (742) - Listeria welshimeri 919 (452) - Brochothrix thermosphacta 847 (283) - Streptococcus pneumoniae FQ26 (590) - Streptococcus pneumoniae 67620 (596) - Streptococcus pneumoniae 15M-1047 (790) -

NOTE: Not active against Pseudomonas fluorescens 378 - Escherichia coli JM109 - Escherichia coli MC1000 - Salmonella paratyphi 554 - Salmonella typhimurium 443 - Salmonella enteritidis 4396

Description

Garvicin ML is synthesized as a 63-amino-acid precursor with a 3-amino-acid leader peptide that is removed by cleavage. Subsequently, a covalent linkage between the N- and C-terminus forms the mature version of this novel 60-amino-acid circular bacteriocin.

Related Entries

Garvicin A

Uniprot and PDB links

UniProt Entry: D2KC49

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=DCC43.
PubMed=21057028;DOI=10.1128/AEM.01173-10
Borrero J., Brede D.A., Skaugen M., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Characterization of Garvicin ML, a Novel Circular Bacteriocin Produced by Lactococcus garvieae DCC43, Isolated from Mallard Ducks (Anas platyrhynchos).", Appl Environ Microbiol. (2010).

[Ref. 2]

NUCLEOTIDE SEQUENCE, STRAIN=DCC43.
Borrero J., Brede D.A., Skaugen M., Diep D.B., Herranz C., Nes I.F., Cintas L.M., Hernandez P.E.
"Characterization of garvicin ML, a novel circular bacteriocin produced by Lactococcus garvieae DCC43, isolated from Mallard ducks (Anas platyrhynchos).", Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.

Gene structure

Gene id	Name	Description	Location
BACGene885	garML	garvicin ML precursor circular bacteriocin; antimicrobial peptide	32..223

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70
| | | | | | |
LVATGMAAGV AKTIVNAVSA GMDIATALSL FSGAFTAAGG IMALIKKYAQ KKLWKQLIAA

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔60	60	garvicin ML. Feature identifier = PRO_5000556423.

Composition

Hydrophobicity

Composition

Formula	C₀ H₀ N₀ O₀ S₀
Absent amino acids	CEHPR
Common amino acids	A
Mass (Da)	6
Net charge	+5
Isoelectric point	10.85
Basic residues	6
Acidic residues	1
Hydrophobic residues	33
Polar residues	15
Aliphatic residues	15
Tiny residues	24
Boman Index	38.05
Hydropathy Index	0.887
Aliphatic Index	115.83
Instability Index	19.35 (stable)
Half Life	Mammalian : 5.5 hour Yeast : 3 min E. coli : 2 min
Extinction Coefficient	6990 M^-1 cm^-1
Absorbance 280nm	118.47

Users comments for Garvicin ML

No comments found