Bacteriocin: Lactocyclicin Q

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Bacteriocin: Lactocyclicin Q

Accession: BAC191

Classification
Class: Unclassified
Genetics
Gene: lycQ
Producer and target organisms

Producer Organism: Lactococcus sp. QU 12 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeLactococcus

Target organismsGram-positive: Lactococcus sp. strain QU 12 (MIC=34.3µM) - Lactococcus lactis subsp. lactis JCM 7638 (MIC=0.141µM) - Lactococcus lactis subsp. lactis ATCC 19435 (MIC=0.141µM) - Lactococcus lactis subsp. lactis IL1403 (MIC=0.141µM) - Lactococcus lactis QU 1 (MIC=0.141µM) - Lactococcus lactis subsp. cremoris ATCC 19275 (MIC=0.283µM) - Lactococcus raffinolactis JCM 5706 (MIC=0.71µM) - Lactobacillus sakei subsp. sakei JCM 1157 (MIC=0.015µM) - Lactobacillus casei subsp. casei JCM 1134 (MIC=2.27µM) - Lactobacillus brevis JCM 1059 (MIC=1.14µM) - Lactobacillus acidophilus JCM 1132 (MIC=2.86µM) - Lactobacillus coryniformis subsp. coryniformis JCM 1164 (MIC=2.86µM) - Lactobacillus kimchii JCM 10707 (MIC=0.71µM) - Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 (MIC=1.03µM) - Weissella cibaria JCM 12495 (MIC=0.71µM) - Pediococcus pentosaceus JCM 5885 (MIC=0.55µM) - Pediococcus pentosaceus JCM 5890 (MIC=2.86µM) - Pediococcus acidilactici JCM 8797 (MIC=2.86µM) - Pediococcus dextrinicus JCM 5887 (MIC=0.141µM) - Enterococcus faecium JCM5804 (MIC=0.71µM) - Enterococcus hirae ATCC 10541 (MIC=0.71µM) - Enterococcus durans NBRC 100479 (MIC=0.71µM) - Enterococcus faecalis JCM 5803 (MIC=0.26µM) - Streptococcus salivarius JCM 5707 (MIC=22.9µM) - Streptococcus bovis JCM 5802 (MIC=22.9µM) - Bacillus coagulans JCM 2257 (MIC=0.015µM) - Bacillus circulans JCM 2504 (MIC=0.031µM) - Bacillus subtilis JCM 1465 (MIC=0.064µM) - Bacillus cereus JCM 2152 (MIC=11.4µM) - Micrococcus luteus IFO 12708 (MIC=5.7µM) - Listeria innocua ATCC 33090 (MIC=1.03µM) - Listeria monocytogenes ATCC BAA-679 (MIC=1.03µM) - Staphylococcus aureus subsp. aureus ATCC 12600 (MIC=91.6µM) -

Gram-negative: Escherichia coli JM109 (MIC=34.3µM) - Escherichia coli NBRC 3301 (MIC=17.3µM) -

NOTE: No activity against Salmonella enterica serovar Typhimurium NBRC 13245 (>312 µM).

Description
BIOPHYSICOCHEMICAL PROPERTIES:
Temperature dependence: Thermostable, activity is retained after incubation at 121C for 15 min.
pH dependence:Stable at 3.0 and 4.0.

lactocyclicin Q propeptide contains a leader peptide (dipeptide) sequence.

STRUCTURE: The cyclic structure of lactocyclicin Q is achieved through a dehydration reaction between L1 and W61.
Uniprot and PDB links

UniProt Entry: B9ZZY0

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=QU 12.
PubMed=19139222;DOI=10.1128/AEM.02299-08
Sawa N., Zendo T., Kiyofuji J., Fujita K., Himeno K., Nakayama J., Sonomoto K.
"Identification and Characterization of Lactocyclicin Q, a Novel Cyclic Bacteriocin Produced by Lactococcus sp. Strain QU 12.", Appl. Environ. Microbiol. 75:1152-1158(2009).
PROTEIN SEQUENCE, FUNCTION AND BIOPHYSICOCHEMICAL PROPERTIES
PubMed=21948835;DOI=10.1128/AEM.06348-11
Masuda Y., Ono H., Kitagawa H., Ito H., Mu F., Sawa N., Zendo T., Sonomoto K.
"Identification and Characterization of Leucocyclicin Q, a Novel Cyclic Bacteriocin Produced by Leuconostoc mesenteroides TK41401.", Appl. Environ. Microbiol. (2011).
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End Note
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BibTeX
Gene structure
genome of Lactocyclicin Q

Gene idNameDescriptionLocation
BACGene884lycQlactocyclicin Q55..246
lycQ
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 
          |          |          |          |          |          |          | 
 LIDHLGAPRW AVDTILGAIA VGNLASWVLA LVPGPGWAVK AGLATAAAIV KHQGKAAAAA W

Wheel representation
wheel representation of Lactocyclicin Q
Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids CEFMY
Common amino acids A
Mass (Da) 6
Net charge +4
Isoelectric point 10.5
Basic residues 6
Acidic residues 2
Hydrophobic residues 38
Polar residues 11
Aliphatic residues 17
Tiny residues 25
Boman Index 45.98
Hydropathy Index 0.826
Aliphatic Index 126.72
Instability Index 8.94 (stable)
Half Life Mammalian : 5.5 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 22000 M-1 cm-1
Absorbance 280nm 366.67

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