Bacteriocin: Lactocyclicin Q
Accession: BAC191
Producer Organism: Lactococcus sp. QU 12 [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismsGram-positive: Lactococcus sp. strain QU 12 (MIC=34.3µM) - Lactococcus lactis subsp. lactis JCM 7638 (MIC=0.141µM) - Lactococcus lactis subsp. lactis ATCC 19435 (MIC=0.141µM) - Lactococcus lactis subsp. lactis IL1403 (MIC=0.141µM) - Lactococcus lactis QU 1 (MIC=0.141µM) - Lactococcus lactis subsp. cremoris ATCC 19275 (MIC=0.283µM) - Lactococcus raffinolactis JCM 5706 (MIC=0.71µM) - Lactobacillus sakei subsp. sakei JCM 1157 (MIC=0.015µM) - Lactobacillus casei subsp. casei JCM 1134 (MIC=2.27µM) - Lactobacillus brevis JCM 1059 (MIC=1.14µM) - Lactobacillus acidophilus JCM 1132 (MIC=2.86µM) - Lactobacillus coryniformis subsp. coryniformis JCM 1164 (MIC=2.86µM) - Lactobacillus kimchii JCM 10707 (MIC=0.71µM) - Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 (MIC=1.03µM) - Weissella cibaria JCM 12495 (MIC=0.71µM) - Pediococcus pentosaceus JCM 5885 (MIC=0.55µM) - Pediococcus pentosaceus JCM 5890 (MIC=2.86µM) - Pediococcus acidilactici JCM 8797 (MIC=2.86µM) - Pediococcus dextrinicus JCM 5887 (MIC=0.141µM) - Enterococcus faecium JCM5804 (MIC=0.71µM) - Enterococcus hirae ATCC 10541 (MIC=0.71µM) - Enterococcus durans NBRC 100479 (MIC=0.71µM) - Enterococcus faecalis JCM 5803 (MIC=0.26µM) - Streptococcus salivarius JCM 5707 (MIC=22.9µM) - Streptococcus bovis JCM 5802 (MIC=22.9µM) - Bacillus coagulans JCM 2257 (MIC=0.015µM) - Bacillus circulans JCM 2504 (MIC=0.031µM) - Bacillus subtilis JCM 1465 (MIC=0.064µM) - Bacillus cereus JCM 2152 (MIC=11.4µM) - Micrococcus luteus IFO 12708 (MIC=5.7µM) - Listeria innocua ATCC 33090 (MIC=1.03µM) - Listeria monocytogenes ATCC BAA-679 (MIC=1.03µM) - Staphylococcus aureus subsp. aureus ATCC 12600 (MIC=91.6µM) -
Gram-negative: Escherichia coli JM109 (MIC=34.3µM) - Escherichia coli NBRC 3301 (MIC=17.3µM) -
NOTE: No activity against Salmonella enterica serovar Typhimurium NBRC 13245 (>312 µM).
Temperature dependence: Thermostable, activity is retained after incubation at 121°C for 15 min.
pH dependence:Stable at 3.0 and 4.0.
lactocyclicin Q propeptide contains a leader peptide (dipeptide) sequence.
STRUCTURE: The cyclic structure of lactocyclicin Q is achieved through a dehydration reaction between L1 and W61.
UniProt Entry: B9ZZY0
PDB EntryUnknown
PubMed=19139222;DOI=10.1128/AEM.02299-08
Sawa N., Zendo T., Kiyofuji J., Fujita K., Himeno K., Nakayama J., Sonomoto K.
"Identification and Characterization of Lactocyclicin Q, a Novel Cyclic Bacteriocin Produced by Lactococcus sp. Strain QU 12.", Appl. Environ. Microbiol. 75:1152-1158(2009).
PubMed=21948835;DOI=10.1128/AEM.06348-11
Masuda Y., Ono H., Kitagawa H., Ito H., Mu F., Sawa N., Zendo T., Sonomoto K.
"Identification and Characterization of Leucocyclicin Q, a Novel Cyclic Bacteriocin Produced by Leuconostoc mesenteroides TK41401.", Appl. Environ. Microbiol. (2011).
| Gene id | Name | Description | Location |
| BACGene884 | lycQ | lactocyclicin Q | 55..246 |
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 | | | | | | | LIDHLGAPRW AVDTILGAIA VGNLASWVLA LVPGPGWAVK AGLATAAAIV KHQGKAAAAA W |
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | CEFMY |
| Common amino acids | A |
| Mass (Da) | 6 |
| Net charge | +4 |
| Isoelectric point | 10.5 |
| Basic residues | 6 |
| Acidic residues | 2 |
| Hydrophobic residues | 38 |
| Polar residues | 11 |
| Aliphatic residues | 17 |
| Tiny residues | 25 |
| Boman Index | 45.98 |
| Hydropathy Index | 0.826 |
| Aliphatic Index | 126.72 |
| Instability Index | 8.94 (stable) |
| Half Life |
Mammalian : 5.5 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 22000 M-1 cm-1 |
| Absorbance 280nm | 366.67 |
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