Bacteriocin: Enterocin Xbeta
Accession: BAC190
Classification
Class: Class IIb
Genetics
Gene: enxB
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsGram-positive: - Enterococcus faecalis JCM 5803 (MIC=813nM) - Enterococcus faecalis OU510 (MIC=813nM) - Enterococcus faecium TUA 1344L (MIC=813nM) - Enterococcus hirae ATCC 10541 (MIC=406nM) - Lactobacillus plantarum ATCC 14917 (MIC=813nM) - Lactobacillus sakei ssp. sakei JCM 1157 (MIC=102nM) - Bacillus circulans JCM 2504 (MIC=3250nM) - Bacillus coagulans JCM 2257 (MIC=3250nM) - Bacillus subtilis JCM 1465 (MIC=3250nM) - Listeria innocua ATCC 33090 (MIC=3250nM)
Description
entXα and entXβ encode a double-glycine-type prepeptide with an 18-residue leader sequence, representing the typical structure of class II bacteriocin prepeptides.
Uniprot and PDB links
UniProt Entry: D7UP04
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
PubMed=20418437;DOI=10.1128/AEM.02264-09
Hu C.-B., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"Enterocin X, a novel two-peptide bacteriocin from Enterococcus faecium KU-B5, has an antibacterial spectrum entirely different from those of its component peptides.", Appl. Environ. Microbiol. 76:4542-4545(2010).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=KU-B5.
Hu C., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"", Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
Hu C., Malaphan W., Zendo T., Nakayama J., Sonomoto K.
"", Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene878 | hypothetical protein ORFX1; hypothetical protein | 298..444 | |
| BACGene879 | hypothetical protein ORFX2; hypothetical protein | complement(434..766) | |
| BACGene880 | hypothetical protein ORFX3; hypothetical protein | complement(763..978) | |
| BACGene881 | enxA | enterocin Xalpha prepeptide Alpha subunit of enterocin X (two-peptide bacteriocin) | 1255..1431 |
| BACGene882 | enxB | enterocin Xbeta Beta subunit of enterocin X (two-peptide bacteriocin) | 1450..1617 |
| BACGene883 | enxB | hypothetical protein ORFX4; hypothetical protein | 1732..1953 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | IAPIIVAGLG YLVKDAWDHS DQIISGFKKG WNGGRRK |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | CEMT |
| Common amino acids | G |
| Mass (Da) | 4 |
| Net charge | +4 |
| Isoelectric point | 10.48 |
| Basic residues | 7 |
| Acidic residues | 3 |
| Hydrophobic residues | 15 |
| Polar residues | 10 |
| Aliphatic residues | 9 |
| Tiny residues | 11 |
| Boman Index | -40.75 |
| Hydropathy Index | -0.197 |
| Aliphatic Index | 97.57 |
| Instability Index | 24.64 (stable) |
| Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 12490 M-1 cm-1 |
| Absorbance 280nm | 346.94 |
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