Bacteriocin: Closticin 574
Accession: BAC187
Classification
Class: Unclassified
Genetics
Gene: cloA
Producer and target organisms
Producer Organism: Clostridium tyrobutyricum [Gram-positive]
Taxonomy: Bacteria
FirmicutesClostridiaClostridialesClostridiaceaeClostridium
Target organismsUnavailable data
Description
closticin 574 is concluded to be initially produced as a preproprotein of 310 amino acid residues. It is subsequently processed to a bacteriocin of 82 amino acids. The protein has an unusually long preproprotein sequence of 228 amino acids.
Uniprot and PDB links
UniProt Entry: Q8GCU9
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=ADRIAT 932.
MEDLINE=22507685;PubMed=12620847;DOI=10.1128/AEM.69.3.1589-1597.2003
Kemperman R., Kuipers A., Karsens H., Nauta A., Kuipers O., Kok J.
"Identification and characterization of two novel clostridial bacteriocins, circularin A and closticin 574.", Appl. Environ. Microbiol. 69:1589-1597(2003).
MEDLINE=22507685;PubMed=12620847;DOI=10.1128/AEM.69.3.1589-1597.2003
Kemperman R., Kuipers A., Karsens H., Nauta A., Kuipers O., Kok J.
"Identification and characterization of two novel clostridial bacteriocins, circularin A and closticin 574.", Appl. Environ. Microbiol. 69:1589-1597(2003).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=ADRIAT 932.
Kemperman R.A., Kuipers A., Karsens H., Nauta A., Kuipers O.P., Kok J.
"", Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
Kemperman R.A., Kuipers A., Karsens H., Nauta A., Kuipers O.P., Kok J.
"", Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene862 | cloA | closticin 574 CloA; bacteriocin precursor | 374..1303 |
| BACGene863 | cloB | CloB | 1388..1675 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | PNWTKIGKCA GSIAWAIGSG LFGGAKLIKI KKYIAELGGL QKAAKLLVGA TTWEEKLHAG GYALINLAAE LTGVAGIQAN CF |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | DMR |
| Common amino acids | A |
| Mass (Da) | 8 |
| Net charge | +6 |
| Isoelectric point | 10 |
| Basic residues | 10 |
| Acidic residues | 4 |
| Hydrophobic residues | 39 |
| Polar residues | 26 |
| Aliphatic residues | 20 |
| Tiny residues | 29 |
| Boman Index | 19.5 |
| Hydropathy Index | 0.389 |
| Aliphatic Index | 109.76 |
| Instability Index | 3.62 (stable) |
| Half Life |
Mammalian : >20 hour Yeast : >20 hour E. coli : ? |
| Extinction Coefficient | 19605 M-1 cm-1 |
| Absorbance 280nm | 242.04 |
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