Bacteriocin: Dysgalacticin
Accession: BAC186
Classification
Class: Unclassified
Genetics
Gene: dysA
Producer and target organisms
Producer Organism: Streptococcus equisimilis [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeStreptococcus
Target organismsGram positive: Streptococcus pyogenes FF22
Description
Dysgalacticin (Synonym: streptococcin G-2580) is the second plasmid-encoded Sec-dependent bacteriocin. Dysgalacticin is a heat-labile 21.5 kDa anionic protein that kills its target without inducing lysis.
MODE OF ACTION:
Dysgalacticin appears to bind to the glucose and mannose trans-porters of the PTS (ref 3). Dysgalacticin was shown to block the up-take of glucose and the nonmetabolizable analog 2-deoxyglucose;it also perturbs the membrane of the cell, causing the dissipation of membrane potential (ref 3).
MALDI-TOF MS : 21 492.5±19.3 Da
MODE OF ACTION:
Dysgalacticin appears to bind to the glucose and mannose trans-porters of the PTS (ref 3). Dysgalacticin was shown to block the up-take of glucose and the nonmetabolizable analog 2-deoxyglucose;it also perturbs the membrane of the cell, causing the dissipation of membrane potential (ref 3).
MALDI-TOF MS : 21 492.5±19.3 Da
Uniprot and PDB links
UniProt Entry: Q14DY1
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=W2580.
PubMed=16804174;DOI=10.1099/mic.0.28823-0
Heng N.C., Ragland N.L., Swe P.M., Baird H.J., Inglis M.A., Tagg J.R., Jack R.W.
"Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. equisimilis.", Microbiology 152:1991-2001(2006).
PubMed=16804174;DOI=10.1099/mic.0.28823-0
Heng N.C., Ragland N.L., Swe P.M., Baird H.J., Inglis M.A., Tagg J.R., Jack R.W.
"Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. equisimilis.", Microbiology 152:1991-2001(2006).
[Ref. 2]
NUCLEOTIDE SEQUENCE, STRAIN=W2580.
Heng N.C.K.
"", Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
Heng N.C.K.
"", Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
MODE OF ACTION
PubMed=19213799;DOI=10.1093/jac/dkn552
Swe P.M., Cook G.M., Tagg J.R., Jack R.W.
"Mode of action of dysgalacticin: a large heat-labile bacteriocin.", J Antimicrob Chemother 2009, 63 (4):679-86.
PubMed=19213799;DOI=10.1093/jac/dkn552
Swe P.M., Cook G.M., Tagg J.R., Jack R.W.
"Mode of action of dysgalacticin: a large heat-labile bacteriocin.", J Antimicrob Chemother 2009, 63 (4):679-86.
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Gene structure
| Gene id | Name | Description | Location |
| BACGene858 | copG | CopG plasmid copy repressor protein | 86..223 |
| BACGene859 | repB | RepB replication initiation protein | 283..909 |
| BACGene860 | dysI | dysgalacticin immunity factor DysI | 902..1075 |
| BACGene861 | dysA | dysgalacticin preprotein DysA; contains 28-aa Sec-dependent signal peptide | complement(1102..1764) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | NETNNFAETQ KEITTNSEAT LTNEDYTKLT SEVKTIYTNL IQYDQTKNKF YVDEDKTEQY YNYDDESIKG VYLMKDSLND ELNNNNSSNY SEIINQKISE IDYVLQGNDI NNLIPSNTRV KRSADFSWIQ RCLEEAWGYA ISLVTLKGII NLFKAGKFEA AAAKLASATA GRIAGMAALF AFVATCGATT VS |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | H |
| Common amino acids | N |
| Mass (Da) | 21505.82 |
| Net charge | -8 |
| Isoelectric point | 4.38 |
| Basic residues | 18 |
| Acidic residues | 26 |
| Hydrophobic residues | 65 |
| Polar residues | 73 |
| Aliphatic residues | 37 |
| Tiny residues | 41 |
| Boman Index | -352.74 |
| Hydropathy Index | -0.447 |
| Aliphatic Index | 80.89 |
| Instability Index | 24.51 (stable) |
| Half Life |
Mammalian : 1.4 hour Yeast : 3 min E. coli : >10 hour |
| Extinction Coefficient | 27515 M-1 cm-1 |
| Absorbance 280nm | 144.06 |
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