Bacteriocin: Dysgalacticin

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Bacteriocin: Dysgalacticin

Accession: BAC186

Classification
Class: Unclassified
Genetics
Gene: dysA
Producer and target organisms
Description
Dysgalacticin (Synonym: streptococcin G-2580) is the second plasmid-encoded Sec-dependent bacteriocin. Dysgalacticin is a heat-labile 21.5 kDa anionic protein that kills its target without inducing lysis.

MODE OF ACTION:
Dysgalacticin appears to bind to the glucose and mannose trans-porters of the PTS (ref 3). Dysgalacticin was shown to block the up-take of glucose and the nonmetabolizable analog 2-deoxyglucose;it also perturbs the membrane of the cell, causing the dissipation of membrane potential (ref 3).

MALDI-TOF MS : 21 492.519.3 Da
Uniprot and PDB links

UniProt Entry: Q14DY1

PDB EntryUnknown

NUCLEOTIDE SEQUENCE, STRAIN=W2580.
PubMed=16804174;DOI=10.1099/mic.0.28823-0
Heng N.C., Ragland N.L., Swe P.M., Baird H.J., Inglis M.A., Tagg J.R., Jack R.W.
"Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. equisimilis.", Microbiology 152:1991-2001(2006).
NUCLEOTIDE SEQUENCE, STRAIN=W2580.
Heng N.C.K.
"", Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
MODE OF ACTION
PubMed=19213799;DOI=10.1093/jac/dkn552
Swe P.M., Cook G.M., Tagg J.R., Jack R.W.
"Mode of action of dysgalacticin: a large heat-labile bacteriocin.", J Antimicrob Chemother 2009, 63 (4):679-86.
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End Note
Reference Manager
BibTeX
Gene structure
genome of Dysgalacticin

Gene idNameDescriptionLocation
BACGene858copGCopG plasmid copy repressor protein86..223
BACGene859repBRepB replication initiation protein283..909
BACGene860dysIdysgalacticin immunity factor DysI902..1075
BACGene861dysAdysgalacticin preprotein DysA; contains 28-aa Sec-dependent signal peptidecomplement(1102..1764)
copGrepBdysIdysA
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 NETNNFAETQ KEITTNSEAT LTNEDYTKLT SEVKTIYTNL IQYDQTKNKF YVDEDKTEQY YNYDDESIKG VYLMKDSLND
 ELNNNNSSNY SEIINQKISE IDYVLQGNDI NNLIPSNTRV KRSADFSWIQ RCLEEAWGYA ISLVTLKGII NLFKAGKFEA
 AAAKLASATA GRIAGMAALF AFVATCGATT VS

Composition
Hydrophobicity

Composition
Formula C0 H0 N0 O0 S0
Absent amino acids H
Common amino acids N
Mass (Da) 21505.82
Net charge -8
Isoelectric point 4.38
Basic residues 18
Acidic residues 26
Hydrophobic residues 65
Polar residues 73
Aliphatic residues 37
Tiny residues 41
Boman Index -352.74
Hydropathy Index -0.447
Aliphatic Index 80.89
Instability Index 24.51 (stable)
Half Life Mammalian : 1.4 hour
Yeast : 3 min
E. coli : >10 hour
Extinction Coefficient 27515 M-1 cm-1
Absorbance 280nm 144.06

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