Bacteriocin: Plantaricin ASM1
Accession: BAC185
Classification
Class: Unclassified
Genetics
Gene: bactA1
Producer and target organisms
Producer Organism: Lactobacillus plantarum [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesLactobacillaceaeLactobacillus
Target organismsUnavailable data
Description
FUNCTION: Bacteriocin with a narrow antibacterial spectrum. Antibacterial activity against the Gram-positive bacteria
L.plantarun, L.pentosus, L.curvatus, L.lindneri, L.mesenteroides and E.faecilis. Lacks antibacterial activity against the Gram-positive bacteria L.brevis, L.sakei, L. lactis, P.acidilactici, B. subtilis, B. cereus, L. monocytogenes and S.aureus, and against the Gram-negative bacteria E.coli and S.typhimurium.
BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:Stable from pH 5.5-8.5
Temperature dependence: Thermostable, activity is retained after incubation at 90degrees Celsius for 15 minutes
SUBCELLULAR LOCATION: Secreted.
PTM: Contains 2 disulfide bonds (Probable).
MASS SPECTROMETRY: Mass=5045.72; Method=MALDI; Range=22-64;
L.plantarun, L.pentosus, L.curvatus, L.lindneri, L.mesenteroides and E.faecilis. Lacks antibacterial activity against the Gram-positive bacteria L.brevis, L.sakei, L. lactis, P.acidilactici, B. subtilis, B. cereus, L. monocytogenes and S.aureus, and against the Gram-negative bacteria E.coli and S.typhimurium.
BIOPHYSICOCHEMICAL PROPERTIES:
pH dependence:Stable from pH 5.5-8.5
Temperature dependence: Thermostable, activity is retained after incubation at 90degrees Celsius for 15 minutes
SUBCELLULAR LOCATION: Secreted.
PTM: Contains 2 disulfide bonds (Probable).
MASS SPECTROMETRY: Mass=5045.72; Method=MALDI; Range=22-64;
Uniprot and PDB links
UniProt Entry: C7G1H4
PDB Entry2MVI resolved by NMR
NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 22-63, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, DISULFIDE BONDS, AND MASS SPECTROMETRY, STRAIN=A-1.
PubMed=19939484;DOI=10.1016/j.ijfoodmicro.2009.10.021
Hata T., Tanaka R., Ohmomo S.
"Isolation and characterization of plantaricin ASM1: a new bacteriocin produced by Lactobacillus plantarum A-1.", Int. J. Food Microbiol. 137:94-99(2010).
PubMed=19939484;DOI=10.1016/j.ijfoodmicro.2009.10.021
Hata T., Tanaka R., Ohmomo S.
"Isolation and characterization of plantaricin ASM1: a new bacteriocin produced by Lactobacillus plantarum A-1.", Int. J. Food Microbiol. 137:94-99(2010).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene852 | hypothetical protein | 352..828 | |
| BACGene853 | hypothetical protein | 692..775 | |
| BACGene854 | hypothetical protein | 788..1636 | |
| BACGene855 | hypothetical protein | 1518..1625 | |
| BACGene856 | bactA1 | bacteriocin A1 | 1718..1912 |
| BACGene857 | bactA1 | hypothetical protein | 1749..1874 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 | | | | | KPAWCWYTLA MCGAGYDSGT CDYMYSHCFG VKHSSGGGGS YHC |
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔43 | 43 | Bacteriocin plantarican ASM1. Feature identifier = PRO_0000394801.
|
| Cross-link | 40↔43 | 4 | Lanthionine (Ser-Cys) (Probable). |
Composition
Hydrophobicity
Composition
| Formula | C0
H0
N0
O0
S0 |
| Absent amino acids | EINQR |
| Common amino acids | G |
| Mass (Da) | 4 |
| Net charge | +3 |
| Isoelectric point | 7.34 |
| Basic residues | 5 |
| Acidic residues | 2 |
| Hydrophobic residues | 8 |
| Polar residues | 25 |
| Aliphatic residues | 2 |
| Tiny residues | 16 |
| Boman Index | -24.71 |
| Hydropathy Index | -0.242 |
| Aliphatic Index | 22.79 |
| Instability Index | 55.58 (unstable) |
| Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 18700 M-1 cm-1 |
| Absorbance 280nm | 445.24 |
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