Bacteriocin: Salivaricin 9

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Bacteriocin: Salivaricin 9

Accession: BAC182

Classification

Class: lantibiotic

Genetics

Gene: sboB; SivA

Producer and target organisms

Producer Organism: Streptococcus salivarius [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsMicrococcus luteus ATCC10240 (MIC= 4 µg/ml) - Micrococcus luteus GAB13 (MIC= 16 µg/ml) - Streptococcus equisimilis ATCC12388 (MIC= 64 µg/ml) - Corynebacterium spp GH17 Corynebacterium spp GH17 (MIC= 8 µg/ml) - Lactococcus lactis ATCC11454 (MIC= 128 µg/ml) - Streptococcus pyogenes ATCC12344 (MIC= 32 µg/ml) - Streptococcus pyogenes ATCC12384 (MIC= 32 µg/ml) -

NOTE: not active against Haemophilus parainfluenza TONEJ11 - Actinomyces naeslundii TG2 - Staphylococcus aureus RF122 - Streptococcus mutans GEJ11 - Bacillus cereus ATCC14579

Description

NOTE: The Sal9 genetic determinants appear generally (but not always) to be megaplasmid-associated, and production seems often to occur in strains that also produce SalA. As is the case for SalA, the production of Sal9 appears to be autoinducible. (Ref 1)

Uniprot and PDB links

UniProt Entry: Q09I51

PDB Entry~~Unknown~~

[Ref. 1]

PROTEIN SEQUENCE, FUNCTION AND MASS SPECTROMETRY
DOI=10.1007/s12602-009-9026-7
Wescombe P.A., Heng N.C.K., Burton J.P., Tagg J.R.
"Something Old and Something New: An Update on the Amazing Repertoire of Bacteriocins Produced by Streptococcus salivarius", Probiotics and Antimicrobial Proteins, 2(1):37-45.

[Ref. 2] | View abstract | Export citation

PROTEIN SEQUENCE, FUNCTION AND MASS SPECTROMETRY
PubMed=21310787;DOI=10.1099/mic.0.044719-0
Wescombe P.A., Upton M., Renault P., Wirawan R.E., Power D., Burton J.P., Chilcott C.N., Tagg J.R.
"Salivaricin 9, a new lantibiotic produced by Streptococcus salivarius.", Microbiology. 157 (5):1290-1299 (2011).

[Ref. 3] | View abstract | Export citation

FUNCTION AND MECHANISM OF ACTION
PubMed=24147072;DOI=10.1371/journal.pone.0077751
Barbour A., Philip K., Muniandy S.
"Enhanced production, purification, characterization and mechanism of action of salivaricin 9 lantibiotic produced by Streptococcus salivarius NU10.", PLoS One 2013, 8 (10):e77751.

Gene structure

Gene id	Name	Description	Location
BACGene843	sivK	SivK putative histidine kinase	complement(1..1210)
BACGene844	sivR	SivR putative response regulator	complement(1230..1919)
BACGene845	sivA	SivA lantibiotic salivaricin 9 prepropeptide	2116..2286
BACGene846	sivM	SivM putative salivaricin 9 modification enzyme	2452..5235
BACGene847	sivT	SivT putative salivaricin 9 ABC transporter; contains an N-terminal protease domainwith motifs characteristic of lantibiotic proteases	5252..6277

Protein sequence

........10 ........20 ........30
| | |
GNGVVLTLTH ECNLATWTKK LKCC

Wheel representation

Composition

Hydrophobicity

Composition

Formula	C₁₁₄ H₁₉₀ N₃₂ O₃₃ S₃
Absent amino acids	DFIMPQRSY
Common amino acids	LT
Mass (Da)	2560
Net charge	+3
Isoelectric point	8.52
Basic residues	4
Acidic residues	1
Hydrophobic residues	8
Polar residues	11
Aliphatic residues	6
Tiny residues	3
Boman Index	-14.06
Hydropathy Index	0.13
Aliphatic Index	93.33
Instability Index	-1.51 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	5625 M^-1 cm^-1
Absorbance 280nm	244.57

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