Bacteriocin: thuricin-17

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Bacteriocin: thuricin-17

Accession: BAC177

Classification

Class: Class IId

Genetics

Gene: tucA1

Producer and target organisms

Producer Organism: Bacillus thuringiensis NEB17 [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Target organismsEscherichia coli MM294 4(pBS42) - Bacillus thuringiensis ssp. thuringiensis HD2 - Bacillus thuringiensis ssp. sotto 4-1 - Bacillus thuringiensis ssp. galleriae HD29 - Bacillus thuringiensis ssp. canadensis HD224 - Bacillus thuringiensis ssp. entomocidus HD10 - Bacillus thuringiensis ssp. morrisoni HD12 - Bacillus thuringiensis ssp. darmstadiensis HD146 (103) - Bacillus thuringiensis ssp. pakistani HD395 - Bacillus thuringiensis ssp. indiana HD521 - Bacillus thuringiensis ssp. tochigiensis HD868 (117-72) - Bacillus thuringiensis ssp. cameroun 273B - Bacillus thuringiensis serovar xiaguangiensis 3397 - Bacillus thuringiensis serovar asturiensis EA 34594 - Bacillus thuringiensis serovar rongseni Scg04-02 - Bacillus thuringiensis ssp. thuringiensis Bt1627 - Bacillus thuringiensis ssp. alesti HD4 - Bacillus cereus T-HT - Bacillus cereus T-HW3 - Bacillus cereus 6A3 StrepR - Bacillus cereus ATCC 14579 - Bacillus licheniformis 9945A - Bacillus licheniformis 749 - Bacillus megaterium ATCC 19213 - Bacillus megaterium QM B1551 - Bacillus pumilus ATCC 7061 - Bacillus KTCC B1 - Bacillus KTCC B2 - Brevibacillus brevis ATCC 8246 - Geobacillus stearothermophilus 10 - Paenibacillus polymyxa ATCC 842 - Paenibacillus dendritiformis C168 - note: not active against Bacillus thuringiensis NEB17 - Bradyrhizobium japonicum 532C - Bradyrhizobium japonicum USDA 3 - Bradyrhizobium japonicum USDA 110 - Escherichia coli JM83 3(pMK3) - Pseudomonas putida NRLL-B-14688 - Ralstonia spp. H16 ATCC 17699 - Serratia proteomaculans 1-102 - Serratia proteomaculans 2-68R - Stenotrophomoas meiltophilia Alfa-nod - Staphylococcus epidermidis ATCC 12228 - Bacillus thuringiensis ssp. kurstaki HD1 - Bacillus thuringiensis ssp. entomocidus HD9 - Bacillus thuringiensis ssp. subtoxicus HD109 - Bacillus licheniformis Alfa-Rhiz - Bacillus pumilus Biosubtyl - Bacillus sphaericus 1593 - Bacillus subtilis NEB 5 - Bacillus subtilis NEB 4 - Bacillus subtilis ssp. subtilis 168 - Aneurinibacillus migulanus NRS-1137T

Uniprot and PDB links

UniProt Entry: B5U2V4

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE.
PubMed=19083012;DOI=10.1007/s00425-008-0870-6
Lee K.D., Gray E.J., Mabood F., Jung W.J., Charles T., Clark S.R., Ly A., Souleimanov A., Zhou X., Smith D.L.
"The class IId bacteriocin thuricin-17 increases plant growth.";", Planta 229:747-755(2009)

[Ref. 2] | View abstract | Export citation

PubMed=16478494;
Gray EJ, Lee KD, Souleimanov AM, Di Falco MR, Zhou X, Ly A, Charles TC, Driscoll BT, Smith DL.
"A novel bacteriocin, thuricin 17, produced by plant growth promoting rhizobacteria strain Bacillus thuringiensis NEB17: isolation and classification.", J Appl Microbiol. 100(3):545-54(2006)

Gene structure

Gene id	Name	Description	Location
BACGene820	thnP	ThnP putative leader peptide-processing serine protease	complement(1..1398)
BACGene821	thnE	ThnE ABC transporter; putative permease protein	complement(1619..2266)
BACGene822	thnD	ThnD ABC transporter; putative ATP-binding protein	complement(2256..3122)
BACGene823	thnR	ThnR GntR family transcriptional regulator; putative response regulator	complement(3119..3502)
BACGene824	thnA1	thurincin H precursor bacteriocin precursor; thurincin H1	3822..3944
BACGene825	thnA2	thurincin H precursor bacteriocin precursor; thurincin H2	4051..4173
BACGene826	thnA3	thurincin H precursor bacteriocin precursor; thurincin H3	4279..4401
BACGene827	thnB	ThnB Fe-S oxidoreductase; radical SAM domain protein; putative bacteriocin processingprotein	4566..5945
BACGene828	thnT	ThnT ABC transporter; putative ABC-family secretion protein	6110..7819
BACGene829	thnI	ThnI putative immunity protein	7854..8141

Protein sequence

........10 ........20 ........30 ........40
| | | |
DWTCWSCLVC AACSVELLNL VTAATGASTA S

Wheel representation

Composition

Hydrophobicity

Composition

Formula	C₁₃₄ H₂₁₂ N₃₄ O₄₅ S₄
Absent amino acids	FHIKMPQRY
Common amino acids	A
Mass (Da)	3166.14
Net charge	-2
Isoelectric point	3.55
Basic residues	0
Acidic residues	2
Hydrophobic residues	15
Polar residues	14
Aliphatic residues	7
Tiny residues	11
Boman Index	7.33
Hydropathy Index	0.96
Aliphatic Index	97.74
Instability Index	35.15 (stable)
Half Life	Mammalian : 1.1 hour Yeast : 3 min E. coli : >10 hour
Extinction Coefficient	11000 M^-1 cm^-1
Absorbance 280nm	375

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