Bacteriocin: Enterocin HF

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PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.STRAIN=HS, and TA29;
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocinproduced by E. faecium HS and E. faecium TA29 isolated from humans andfish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtK
PROTEIN SEQUENCE OF 16-58, FUNCTION, AND MASS SPECTROMETRY, STRAIN=HS, and TA29.
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin produced by E. faecium HS and E. faecium TA29 isolated from humans and fish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtKB.
SOLUTION STRUCTURE
PubMed=26585399;DOI=10.1021/acs.jafc.5b03882
Arbulu S., Lohans C.T., Van Belkum M.J., Cintas L.M., Herranz C., Vederas J.C., Hernández P.E.
"Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.", J Agric Food Chem 2015, ():.
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BibTeX
Gene structure
genome of Enterocin HF

Gene idNameDescriptionLocation
BACGene812entHFenterocin HF pediocin-like bacteriocin; EntHF198..374
BACGene813entHFputative EntHF ABC-transporter ORF2518..2536
BACGene814entHFputative EntHF immunity protein ORF32596..2892
entHFentHFentHF
Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 KYYGNGVSCN KKGCSVDWGK AIGIIGNNAA ANLTTGGKAG WKG

Wheel representation
wheel representation of Enterocin HF
3D Structure
Protein sequence annotations
Features of Enterocin HF


FeaturePosition(s)LengthDescription
PEPTIDE1↔4343
Enterocin HF.
Feature identifier =
DISULFID9↔14 6
STRAND2
STRAND6↔9 4
STRAND13↔16 4
HELIX19↔3012
Composition
Hydrophobicity

Composition
Formula C188 H295 N55 O57 S3
Absent amino acids EFHMPQR
Common amino acids G
Mass (Da) 4328.1
Net charge +5
Isoelectric point 10.12
Basic residues 6
Acidic residues 1
Hydrophobic residues 13
Polar residues 23
Aliphatic residues 6
Tiny residues 17
Boman Index -34.01
Hydropathy Index -0.374
Aliphatic Index 61.4
Instability Index 4.29 (stable)
Half Life Mammalian : 1.3 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 14105 M-1 cm-1
Absorbance 280nm 335.83

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