Bacteriocin: Enterocin HF
Accession: BAC175
Classification
Class: class IIa
Genetics
Gene: EntHF
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacilli
Lactobacillales
Enterococcaceae
Enterococcus
Target organismsUnavailable data
Uniprot and PDB links
PDB Entry2N4K resolved by NMR
[Ref. 1]
PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.STRAIN=HS, and TA29;
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocinproduced by E. faecium HS and E. faecium TA29 isolated from humans andfish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtK
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocinproduced by E. faecium HS and E. faecium TA29 isolated from humans andfish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtK
[Ref. 2]
PROTEIN SEQUENCE OF 16-58, FUNCTION, AND MASS SPECTROMETRY, STRAIN=HS, and TA29.
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin produced by E. faecium HS and E. faecium TA29 isolated from humans and fish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtKB.
Almeida T., Brandao A., Campanero C., Igrejas G., Torres C., Herandez P.E., Poeta P., Cintas L.M., Herranz C.
"Amino acid sequence of Enterocin-HF, a new pediocin-like bacteriocin produced by E. faecium HS and E. faecium TA29 isolated from humans and fish (thin-lipped grey mullet; Liza ramada) in Portugal.", Submitted (JAN-2009) to UniProtKB.
SOLUTION STRUCTURE
PubMed=26585399;DOI=10.1021/acs.jafc.5b03882
Arbulu S., Lohans C.T., Van Belkum M.J., Cintas L.M., Herranz C., Vederas J.C., Hernández P.E.
"Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.", J Agric Food Chem 2015, ():.
PubMed=26585399;DOI=10.1021/acs.jafc.5b03882
Arbulu S., Lohans C.T., Van Belkum M.J., Cintas L.M., Herranz C., Vederas J.C., Hernández P.E.
"Solution Structure of Enterocin HF, an Antilisterial Bacteriocin Produced by Enterococcus faecium M3K31.", J Agric Food Chem 2015, ():.
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Gene structure
Gene id | Name | Description | Location |
BACGene812 | entHF | enterocin HF pediocin-like bacteriocin; EntHF | 198..374 |
BACGene813 | entHF | putative EntHF ABC-transporter ORF2 | 518..2536 |
BACGene814 | entHF | putative EntHF immunity protein ORF3 | 2596..2892 |
Protein sequence
........10 ........20 ........30 ........40 ........50 | | | | | KYYGNGVSCN KKGCSVDWGK AIGIIGNNAA ANLTTGGKAG WKG |
Wheel representation
3D Structure
Protein sequence annotations
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔43 | 43 | Enterocin HF.
Feature identifier = |
DISULFID | 9↔14 | 6 | |
STRAND | 2 | ||
STRAND | 6↔9 | 4 | |
STRAND | 13↔16 | 4 | |
HELIX | 19↔30 | 12 |
Composition
Hydrophobicity
Composition
Formula | C188
H295
N55
O57
S3 |
Absent amino acids | EFHMPQR |
Common amino acids | G |
Mass (Da) | 4328.1 |
Net charge | +5 |
Isoelectric point | 10.12 |
Basic residues | 6 |
Acidic residues | 1 |
Hydrophobic residues | 13 |
Polar residues | 23 |
Aliphatic residues | 6 |
Tiny residues | 17 |
Boman Index | -34.01 |
Hydropathy Index | -0.374 |
Aliphatic Index | 61.4 |
Instability Index | 4.29 (stable) |
Half Life |
Mammalian : 1.3 hour Yeast : 3 min E. coli : 2 min |
Extinction Coefficient | 14105 M-1 cm-1 |
Absorbance 280nm | 335.83 |
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