Bacteriocin: Colicin-Ib

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Bacteriocin: Colicin-Ib

Accession: BAC173

Classification

Class: ~~Unclassified~~

Genetics

Gene: cib

Producer and target organisms

Producer Organism: Escherichia coli [Gram-negative]

Taxonomy: BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Target organisms~~Unavailable data~~

Description

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.

Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.

Uniprot and PDB links

UniProt Entry: P04479

PDB Entry~~Unknown~~

[Ref. 1]

ERRATUM.
Varley J.M., Boulnois G.J.
"", Nucleic Acids Res. 12:8748-8748(1984)

[Ref. 2] | View abstract | Export citation

[3]NUCLEOTIDE SEQUENCE [GENOMIC DNA]
MEDLINE=85014128;PubMed=6091036;DOI=10.1093/nar/12.17.6727
Varley J.M., Boulnois G.J.
"Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.", Nucleic Acids Res. 12:6727-6739(1984)

[Ref. 3] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40
MEDLINE=84264487;PubMed=6204975;
Mankovich J.A., Lai P.H., Gokul N., Konisky J.
"Organization of the colicin Ib gene. Promoter structure and immunitydomain.", J. Biol. Chem. 259:8764-8768(1984)

[Ref. 4] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib.", J. Bacteriol. 168:228-236(1986)

Gene structure

Gene id	Name	Description	Location
BACGene808		ORF1	1..651
BACGene809		colicin Ib structural protein	930..2810
BACGene810		colicin Ib immunity protein	complement(2828..3175)
BACGene811		ORF2	3333..3641

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80
| | | | | | | |
MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW VDDSPTRSDI EKRDKEITAY
KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT
ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL
GQQTRNDRAI SEARNKLSSV TESLKTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS STNHSIVVSG DPRFAGTIKI
TTSAVIDNRA NLNYLLTHSG LDYKRNILND RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAINSARNN
VSARTNEQKH ANDALNALLK EKENIRSQLA DINQKIAEEK RKRDEINMVK DAIKLTSDFY RTIYDEFGKQ ASELAKELAS
VSQGKQIKSV DDALNAFDKF RNNLNKKYNI QDRMAISKAL EAINQVHMAE NFKLFSKAFG FTGKVIERYD VAVELQKAVK
TDNWRPFFVK LESLAAGRAA SAVTAWAFSV MLGTPVGILG FAIIMAAVSA LVNDKFIEQV NKLIGI

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔626	626	Colicin-Ib. Feature identifier = PRO_0000218676.
TRANSMEM	588↔612	25	Helical; (Potential).

Composition

Hydrophobicity

Composition

Formula	C₃₀₄₉ H₄₉₃₅ N₉₀₃ O₉₆₂ S₉
Absent amino acids	C
Common amino acids	A
Mass (Da)	69952.45
Net charge	+14
Isoelectric point	9.6
Basic residues	103
Acidic residues	89
Hydrophobic residues	223
Polar residues	161
Aliphatic residues	122
Tiny residues	146
Boman Index	-1512.06
Hydropathy Index	-0.61
Aliphatic Index	82.88
Instability Index	37.17 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	52370 M^-1 cm^-1
Absorbance 280nm	83.79

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