Bacteriocin: Colicin-Ib
Accession: BAC173
Classification
Class: Unclassified
Genetics
Gene: cib
Producer and target organisms
Producer Organism: Escherichia coli [Gram-negative]
Taxonomy: BacteriaProteobacteria
Gammaproteobacteria
Enterobacteriales
Enterobacteriaceae
Escherichia
Target organismsUnavailable data
Description
This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
Uniprot and PDB links
UniProt Entry: P04479
PDB EntryUnknown
[Ref. 1]
[3]NUCLEOTIDE SEQUENCE [GENOMIC DNA]
MEDLINE=85014128;PubMed=6091036;DOI=10.1093/nar/12.17.6727
Varley J.M., Boulnois G.J.
"Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.", Nucleic Acids Res. 12:6727-6739(1984)
MEDLINE=85014128;PubMed=6091036;DOI=10.1093/nar/12.17.6727
Varley J.M., Boulnois G.J.
"Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.", Nucleic Acids Res. 12:6727-6739(1984)
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40
MEDLINE=84264487;PubMed=6204975;
Mankovich J.A., Lai P.H., Gokul N., Konisky J.
"Organization of the colicin Ib gene. Promoter structure and immunitydomain.", J. Biol. Chem. 259:8764-8768(1984)
MEDLINE=84264487;PubMed=6204975;
Mankovich J.A., Lai P.H., Gokul N., Konisky J.
"Organization of the colicin Ib gene. Promoter structure and immunitydomain.", J. Biol. Chem. 259:8764-8768(1984)
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib.", J. Bacteriol. 168:228-236(1986)
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib.", J. Bacteriol. 168:228-236(1986)
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Gene structure
Gene id | Name | Description | Location |
BACGene808 | ORF1 | 1..651 | |
BACGene809 | colicin Ib structural protein | 930..2810 | |
BACGene810 | colicin Ib immunity protein | complement(2828..3175) | |
BACGene811 | ORF2 | 3333..3641 |
Protein sequence
........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW VDDSPTRSDI EKRDKEITAY KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL GQQTRNDRAI SEARNKLSSV TESLKTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS STNHSIVVSG DPRFAGTIKI TTSAVIDNRA NLNYLLTHSG LDYKRNILND RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAINSARNN VSARTNEQKH ANDALNALLK EKENIRSQLA DINQKIAEEK RKRDEINMVK DAIKLTSDFY RTIYDEFGKQ ASELAKELAS VSQGKQIKSV DDALNAFDKF RNNLNKKYNI QDRMAISKAL EAINQVHMAE NFKLFSKAFG FTGKVIERYD VAVELQKAVK TDNWRPFFVK LESLAAGRAA SAVTAWAFSV MLGTPVGILG FAIIMAAVSA LVNDKFIEQV NKLIGI |
Protein sequence annotations
Feature | Position(s) | Length | Description |
CHAIN | 1↔626 | 626 | Colicin-Ib. Feature identifier = PRO_0000218676. |
TRANSMEM | 588↔612 | 25 | Helical; (Potential). |
Composition
Hydrophobicity
Composition
Formula | C3049
H4935
N903
O962
S9 |
Absent amino acids | C |
Common amino acids | A |
Mass (Da) | 69952.45 |
Net charge | +14 |
Isoelectric point | 9.6 |
Basic residues | 103 |
Acidic residues | 89 |
Hydrophobic residues | 223 |
Polar residues | 161 |
Aliphatic residues | 122 |
Tiny residues | 146 |
Boman Index | -1512.06 |
Hydropathy Index | -0.61 |
Aliphatic Index | 82.88 |
Instability Index | 37.17 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 52370 M-1 cm-1 |
Absorbance 280nm | 83.79 |
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