Bacteriocin: Colicin-Ib

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Bacteriocin: Colicin-Ib

Accession: BAC173

Classification
Class: Unclassified
Genetics
Gene: cib
Producer and target organisms
Description
This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.

Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
Uniprot and PDB links

UniProt Entry: P04479

PDB EntryUnknown

ERRATUM.
Varley J.M., Boulnois G.J.
"", Nucleic Acids Res. 12:8748-8748(1984)
[3]NUCLEOTIDE SEQUENCE [GENOMIC DNA]
MEDLINE=85014128;PubMed=6091036;DOI=10.1093/nar/12.17.6727
Varley J.M., Boulnois G.J.
"Analysis of a cloned colicin Ib gene: complete nucleotide sequence and implications for regulation of expression.", Nucleic Acids Res. 12:6727-6739(1984)
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40
MEDLINE=84264487;PubMed=6204975;
Mankovich J.A., Lai P.H., Gokul N., Konisky J.
"Organization of the colicin Ib gene. Promoter structure and immunitydomain.", J. Biol. Chem. 259:8764-8768(1984)
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib.", J. Bacteriol. 168:228-236(1986)
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Gene structure
genome of Colicin-Ib

Gene idNameDescriptionLocation
BACGene808ORF11..651
BACGene809colicin Ib structural protein930..2810
BACGene810colicin Ib immunity proteincomplement(2828..3175)
BACGene811ORF23333..3641
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW VDDSPTRSDI EKRDKEITAY
 KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT
 ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL
 GQQTRNDRAI SEARNKLSSV TESLKTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS STNHSIVVSG DPRFAGTIKI
 TTSAVIDNRA NLNYLLTHSG LDYKRNILND RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAINSARNN
 VSARTNEQKH ANDALNALLK EKENIRSQLA DINQKIAEEK RKRDEINMVK DAIKLTSDFY RTIYDEFGKQ ASELAKELAS
 VSQGKQIKSV DDALNAFDKF RNNLNKKYNI QDRMAISKAL EAINQVHMAE NFKLFSKAFG FTGKVIERYD VAVELQKAVK
 TDNWRPFFVK LESLAAGRAA SAVTAWAFSV MLGTPVGILG FAIIMAAVSA LVNDKFIEQV NKLIGI

Protein sequence annotations

FeaturePosition(s)LengthDescription
CHAIN1↔626626
Colicin-Ib.
Feature identifier = PRO_0000218676.
TRANSMEM588↔61225Helical; (Potential).
Composition
Hydrophobicity

Composition
Formula C3049 H4935 N903 O962 S9
Absent amino acids C
Common amino acids A
Mass (Da) 69952.45
Net charge +14
Isoelectric point 9.6
Basic residues 103
Acidic residues 89
Hydrophobic residues 223
Polar residues 161
Aliphatic residues 122
Tiny residues 146
Boman Index -1512.06
Hydropathy Index -0.61
Aliphatic Index 82.88
Instability Index 37.17 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 52370 M-1 cm-1
Absorbance 280nm 83.79

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