Bacteriocin: Colicin-Ia

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Bacteriocin: Colicin-Ia

Accession: BAC172

Classification
Class: Unclassified
Genetics
Gene: cia
Producer and target organisms
Description
This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.

Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 23-624.PLASMID=ColIa-CA53
MEDLINE=97162210;PubMed=9009197;DOI=10.1038/385461a0
Wiener M., Freymann D., Ghosh P., Stroud R.M.
"Crystal structure of colicin Ia.", Nature 385:461-464(1997)
NUCLEOTIDE SEQUENCE [GENOMIC DNA].PLASMID=ColIa-EC28
MEDLINE=95062249;PubMed=7972047;DOI=10.1073/pnas.91.23.11276
Riley M.A., Tan Y., Wang J.
"Nucleotide polymorphism in colicin E1 and Ia plasmids from naturalisolates of Escherichia coli", Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994)
SEQUENCE REVISION TO 304

Konisky J.
"", Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
NUCLEOTIDE SEQUENCE [GENOMIC DNA].PLASMID=ColIa-CA53
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib", J. Bacteriol. 168:228-236(1986)
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Gene structure
genome of Colicin-Ia

Gene idNameDescriptionLocation
BACGene804unknown ORF11..651
BACGene805colicin Ia structural protein930..2810
BACGene806colicin Ia immunity proteincomplement(2832..3167)
BACGene807unknown ORF23335..3643
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW VDDSPTRSDI EKRDKEITAY
 KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT
 ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL
 GQQTRNDRAI SEARNKLSSV TESLNTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS STNHSIVVSG DPRFAGTIKI
 TTSAVIDNRA NLNYLLSHSG LDYKRNILND RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAVNSARNN
 LSARTNEQKH ANDALNALLK EKENIRNQLS GINQKIAEEK RKQDELKATK DAINFTTEFL KSVSEKYGAK AEQLAREMAG
 QAKGKKIRNV EEALKTYEKY RADINKKINA KDRAAIAAAL ESVKLSDISS NLNRFSRGLG YAGKFTSLAD WITEFGKAVR
 TENWRPLFVK TETIIAGNAA TALVALVFSI LTGSALGIIG YGLLMAVTGA LIDESLVEKA NKFWGI

3D Structure
Protein sequence annotations

FeaturePosition(s)LengthDescription
CHAIN1↔626626
Colicin-Ia.
Feature identifier = PRO_0000218675.
TRANSMEM580↔59415Helical.
TRANSMEM597↔61216Helical.
REGION23↔225203Translocation (T).
REGION282↔385104Receptor-binding (R).
REGION450↔626177Channel (C).
CONFLICT304H -> D (in Ref. 3; AAA59396).
HELIX48↔514
HELIX67↔12155
HELIX126↔16843
STRAND172↔1743
HELIX176↔282107
HELIX285↔2917
TURN293↔2953
STRAND298↔30811
TURN312↔3143
STRAND316↔32611
HELIX329↔3379
HELIX340↔3467
HELIX359↔38325
HELIX470↔48011
TURN481↔4844
HELIX490↔49910
HELIX501↔5044
HELIX511↔52111
HELIX525↔53915
HELIX548↔56013
HELIX565↔5717
TURN572↔5743
HELIX580↔59112
HELIX597↔61216
HELIX614↔6218
Composition
Hydrophobicity

Composition
Formula C3019 H4909 N901 O963 S6
Absent amino acids C
Common amino acids A
Mass (Da) 69457.7
Net charge +15
Isoelectric point 9.72
Basic residues 103
Acidic residues 88
Hydrophobic residues 219
Polar residues 174
Aliphatic residues 120
Tiny residues 155
Boman Index -1515.11
Hydropathy Index -0.64
Aliphatic Index 82.91
Instability Index 34 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 59360 M-1 cm-1
Absorbance 280nm 94.98

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