Bacteriocin: Colicin-Ia
Accession: BAC172
Classification
Class: Unclassified
Genetics
Gene: cia
Producer and target organisms
Producer Organism: Escherichia coli [Gram-negative]
Taxonomy: Bacteria
ProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Target organismsUnavailable data
Description
This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
Colicins are polypeptide toxins produced by and active against Escherichia coli and closely related bacteria.
Uniprot and PDB links
UniProt Entry: P06716
X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 23-624.PLASMID=ColIa-CA53
MEDLINE=97162210;PubMed=9009197;DOI=10.1038/385461a0
Wiener M., Freymann D., Ghosh P., Stroud R.M.
"Crystal structure of colicin Ia.", Nature 385:461-464(1997)
MEDLINE=97162210;PubMed=9009197;DOI=10.1038/385461a0
Wiener M., Freymann D., Ghosh P., Stroud R.M.
"Crystal structure of colicin Ia.", Nature 385:461-464(1997)
NUCLEOTIDE SEQUENCE [GENOMIC DNA].PLASMID=ColIa-EC28
MEDLINE=95062249;PubMed=7972047;DOI=10.1073/pnas.91.23.11276
Riley M.A., Tan Y., Wang J.
"Nucleotide polymorphism in colicin E1 and Ia plasmids from naturalisolates of Escherichia coli", Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994)
MEDLINE=95062249;PubMed=7972047;DOI=10.1073/pnas.91.23.11276
Riley M.A., Tan Y., Wang J.
"Nucleotide polymorphism in colicin E1 and Ia plasmids from naturalisolates of Escherichia coli", Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994)
[Ref. 3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].PLASMID=ColIa-CA53
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib", J. Bacteriol. 168:228-236(1986)
MEDLINE=87008385;PubMed=3531169;
Mankovich J.A., Hsu C.-H., Konisky J.
"DNA and amino acid sequence analysis of structural and immunity genesof colicins Ia and Ib", J. Bacteriol. 168:228-236(1986)
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Gene structure
| Gene id | Name | Description | Location |
| BACGene804 | unknown ORF1 | 1..651 | |
| BACGene805 | colicin Ia structural protein | 930..2810 | |
| BACGene806 | colicin Ia immunity protein | complement(2832..3167) | |
| BACGene807 | unknown ORF2 | 3335..3643 |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | MSDPVRITNP GAESLGYDSD GHEIMAVDIY VNPPRVDVFH GTPPAWSSFG NKTIWGGNEW VDDSPTRSDI EKRDKEITAY KNTLSAQQKE NENKRTEAGK RLSAAIAARE KDENTLKTLR AGNADAADIT RQEFRLLQAE LREYGFRTEI AGYDALRLHT ESRMLFADAD SLRISPREAR SLIEQAEKRQ KDAQNADKKA ADMLAEYERR KGILDTRLSE LEKNGGAALA VLDAQQARLL GQQTRNDRAI SEARNKLSSV TESLNTARNA LTRAEQQLTQ QKNTPDGKTI VSPEKFPGRS STNHSIVVSG DPRFAGTIKI TTSAVIDNRA NLNYLLSHSG LDYKRNILND RNPVVTEDVE GDKKIYNAEV AEWDKLRQRL LDARNKITSA ESAVNSARNN LSARTNEQKH ANDALNALLK EKENIRNQLS GINQKIAEEK RKQDELKATK DAINFTTEFL KSVSEKYGAK AEQLAREMAG QAKGKKIRNV EEALKTYEKY RADINKKINA KDRAAIAAAL ESVKLSDISS NLNRFSRGLG YAGKFTSLAD WITEFGKAVR TENWRPLFVK TETIIAGNAA TALVALVFSI LTGSALGIIG YGLLMAVTGA LIDESLVEKA NKFWGI |
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔626 | 626 | Colicin-Ia. Feature identifier = PRO_0000218675. |
| TRANSMEM | 580↔594 | 15 | Helical. |
| TRANSMEM | 597↔612 | 16 | Helical. |
| REGION | 23↔225 | 203 | Translocation (T). |
| REGION | 282↔385 | 104 | Receptor-binding (R). |
| REGION | 450↔626 | 177 | Channel (C). |
| CONFLICT | 304 | H -> D (in Ref. 3; AAA59396). | |
| HELIX | 48↔51 | 4 | |
| HELIX | 67↔121 | 55 | |
| HELIX | 126↔168 | 43 | |
| STRAND | 172↔174 | 3 | |
| HELIX | 176↔282 | 107 | |
| HELIX | 285↔291 | 7 | |
| TURN | 293↔295 | 3 | |
| STRAND | 298↔308 | 11 | |
| TURN | 312↔314 | 3 | |
| STRAND | 316↔326 | 11 | |
| HELIX | 329↔337 | 9 | |
| HELIX | 340↔346 | 7 | |
| HELIX | 359↔383 | 25 | |
| HELIX | 470↔480 | 11 | |
| TURN | 481↔484 | 4 | |
| HELIX | 490↔499 | 10 | |
| HELIX | 501↔504 | 4 | |
| HELIX | 511↔521 | 11 | |
| HELIX | 525↔539 | 15 | |
| HELIX | 548↔560 | 13 | |
| HELIX | 565↔571 | 7 | |
| TURN | 572↔574 | 3 | |
| HELIX | 580↔591 | 12 | |
| HELIX | 597↔612 | 16 | |
| HELIX | 614↔621 | 8 |
Composition
Hydrophobicity
Composition
| Formula | C3019
H4909
N901
O963
S6 |
| Absent amino acids | C |
| Common amino acids | A |
| Mass (Da) | 69457.7 |
| Net charge | +15 |
| Isoelectric point | 9.72 |
| Basic residues | 103 |
| Acidic residues | 88 |
| Hydrophobic residues | 219 |
| Polar residues | 174 |
| Aliphatic residues | 120 |
| Tiny residues | 155 |
| Boman Index | -1515.11 |
| Hydropathy Index | -0.64 |
| Aliphatic Index | 82.91 |
| Instability Index | 34 (stable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 59360 M-1 cm-1 |
| Absorbance 280nm | 94.98 |
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