Bacteriocin: Thiocillin (Micrococcin P1) (Micrococcin P2) (Thiocillin I) (Thiocillin II) (Thiocillin III) (Thiocillin IV) (Antibiotic YM-266183) (Antibiotic YM-266184)

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Bacteriocin: Thiocillin (Micrococcin P1) (Micrococcin P2) (Thiocillin I) (Thiocillin II) (Thiocillin III) (Thiocillin IV) (Antibiotic YM-266183) (Antibiotic YM-266184)

Accession: BAC171

Classification

Class: ~~Unclassified~~

Genetics

Gene: ~~Unidentified~~

Producer and target organisms

Producer Organism: Bacillus cereus (strain ATCC 14579 / DSM 31) [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Target organismsBacillus subtilis PCI219 - Bacillus anthracis - Staphylococcus aureus FDA209P - Staphylococcus aureus Smith - Staphylococcus aureus 80257 - Streptococcus pyogenes C-203 - Streptococcus pneumoniae type D -

note: not active against Gram-negative bacteria

Uniprot and PDB links

UniProt Entry: Q812G9

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

POST-TRANSLATIONAL MODIFICATIONS.
PubMed=19196969;DOI=10.1073/pnas.0900008106
Brown L.C.W., Acker M.G., Clardy J., Walsh C.T., Fischbach M.A.;"
"Thirteen posttranslational modifications convert a 14-residue peptideinto the antibiotic thiocillin.";", Proc. Natl. Acad. Sci. U.S.A. 106:2549-2553(2009)

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
MEDLINE=22608415;PubMed=12721630;DOI=10.1038/nature01582
Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V., Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M., Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G., Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.
"Genome sequence of Bacillus cereus and comparative analysis withBacillus anthracis", Nature 423:87-91(2003)

[Ref. 3] | View abstract | Export citation

PubMed=819410;
Shoji, J., Hinoo, H., Wakisaka, Y., Koizumi, K., and Mayama, M.
"Isolation of three new antibiotics, thiocillins I, II and III, related to micrococcin P. Studies on antibiotics from the genus Bacillus.", J. Antibiot. (Tokyo) 29,366-374(1976)

Protein sequence

........10 ........20
| |
SCTTCVCTCS CCTT

Wheel representation

Protein sequence annotations

Features of Thiocillin (Micrococcin P1) (Micrococcin P2) (Thiocillin I) (Thiocillin II) (Thiocillin III) (Thiocillin IV) (Antibiotic YM-266183) (Antibiotic YM-266184)

Feature	Position(s)	Length	Description
PEPTIDE	1↔14	14	Thiocillin. Feature identifier = PRO_0000363166.
Modified residue	4		(Z)-2,3-didehydrobutyrine.
Modified residue	6		3-hydroxyvaline; partial.
Modified residue	8		O-methylthreonine; partial.
Modified residue	13		(Z)-2,3-didehydrobutyrine.
Modified residue	14		1-amino-2-propanone; alternate.
Modified residue	14		Decarboxylated threonine; alternate.
Cross-link	1↔10	10	Pyridine-2,5-dicarboxylic acid (Ser-Ser)(with C-47).
Cross-link	1↔9	9	Pyridine-2,5-dicarboxylic acid (Ser-Cys)(with S-48).
Cross-link	1↔2	2	Thiazole-4-carboxylic acid (Ser-Cys).
Cross-link	4↔5	2	Thiazole-4-carboxylic acid (Thr-Cys).
Cross-link	6↔7	2	Thiazole-4-carboxylic acid (Val-Cys).
Cross-link	8↔9	2	Thiazole-4-carboxylic acid (Thr-Cys).
Cross-link	10↔11	2	Thiazole-4-carboxylic acid (Ser-Cys).
Cross-link	11↔12	2	Thiazole-4-carboxylic acid (Cys-Cys).

Composition

Hydrophobicity

Composition

Formula	C₄₉ H₈₆ N₁₄ O₂₂ S₆
Absent amino acids	ADEFGHIKLMNPQRWY
Common amino acids	C
Mass (Da)	1433.89
Net charge	0
Isoelectric point	5.61
Basic residues	0
Acidic residues	0
Hydrophobic residues	1
Polar residues	13
Aliphatic residues	1
Tiny residues	2
Boman Index	-7.93
Hydropathy Index	1.01
Aliphatic Index	20.71
Instability Index	120.33 (unstable)
Half Life	Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	0 M^-1 cm^-1
Absorbance 280nm	28.85

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