Bacteriocin: Reutericin 6

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Bacteriocin: Reutericin 6

Accession: BAC168

Class: class II
Gene: gaaA
Producer and target organisms

Producer Organism: Lactobacillus reuteri LA6 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesLactobacillaceaeLactobacillus

Target organismsLactobacillus delbrueckii subsp. bulgaricus JCM1002

Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatography. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacillus gasseri LA39 which was isolated from the faeces of the same human infant at 4 months old. Reutericin 6 was shown to be a class II and cyclic bacteriocin. PCR amplification on the chromosome DNA of L. reuteri LA6 as a template with the primers based on the DNA sequences cloned (gassericin A and acidocin B from Lactobacillus acidophilus M46) and sequencing revealed that L. reuteri LA6 has the structural gene for gassericin A with no variations among those primers. These results indicate that a bacteriocin of the same structure has been produced by different lactobacilli species isolated from the same infant.

gassericin A inhibited the growth of L. reuteri LA6, but reutericin 6, which has a narrower spectrum than gassericin A, did not inhibit the growth of Lactobacillus gasseri LA39.
Uniprot and PDB links

UniProt Entry: No entry found

PDB EntryUnknown

Kawai, Y., Y. Ishii, K. Uemura, H. Kitazawa, T. Saito, and T. Itoh.
"Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from feces of the same human infant produce identical cyclic bacteriocin.", Food Microbiol. 18:407-415 (2001).
End Note
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Protein sequence
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Wheel representation
wheel representation of Reutericin 6

Formula C261 H411 N67 O72 S1
Absent amino acids CEN
Common amino acids A
Mass (Da) 5672.18
Net charge +1
Isoelectric point 7.54
Basic residues 3
Acidic residues 2
Hydrophobic residues 35
Polar residues 15
Aliphatic residues 13
Tiny residues 27
Boman Index 47.31
Hydropathy Index 0.997
Aliphatic Index 31.62
Instability Index 3.73 (stable)
Half Life Mammalian : 20 hour
Yeast : 30 min
E. coli : >10 hour
Extinction Coefficient 19490 M-1 cm-1
Absorbance 280nm 219.12

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