Bacteriocin: Reutericin 6
Accession: BAC168
Classification
Class: class II
Genetics
Gene: gaaA
Producer and target organisms
Producer Organism: Lactobacillus reuteri LA6 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Lactobacillaceae
Lactobacillus
Target organismsLactobacillus delbrueckii subsp. bulgaricus JCM1002
Description
STRUCTURE:
Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatography. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacillus gasseri LA39 which was isolated from the faeces of the same human infant at 4 months old. Reutericin 6 was shown to be a class II and cyclic bacteriocin. PCR amplification on the chromosome DNA of L. reuteri LA6 as a template with the primers based on the DNA sequences cloned (gassericin A and acidocin B from Lactobacillus acidophilus M46) and sequencing revealed that L. reuteri LA6 has the structural gene for gassericin A with no variations among those primers. These results indicate that a bacteriocin of the same structure has been produced by different lactobacilli species isolated from the same infant.
ACTIVITY:
gassericin A inhibited the growth of L. reuteri LA6, but reutericin 6, which has a narrower spectrum than gassericin A, did not inhibit the growth of Lactobacillus gasseri LA39.
Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatography. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacillus gasseri LA39 which was isolated from the faeces of the same human infant at 4 months old. Reutericin 6 was shown to be a class II and cyclic bacteriocin. PCR amplification on the chromosome DNA of L. reuteri LA6 as a template with the primers based on the DNA sequences cloned (gassericin A and acidocin B from Lactobacillus acidophilus M46) and sequencing revealed that L. reuteri LA6 has the structural gene for gassericin A with no variations among those primers. These results indicate that a bacteriocin of the same structure has been produced by different lactobacilli species isolated from the same infant.
ACTIVITY:
gassericin A inhibited the growth of L. reuteri LA6, but reutericin 6, which has a narrower spectrum than gassericin A, did not inhibit the growth of Lactobacillus gasseri LA39.
Uniprot and PDB links
UniProt Entry: No entry found
PDB EntryUnknown
[Ref. 1]
NUCLEOTIDE SEQUENCE
DOI=10.1006/fmic.2001.0412
Kawai, Y., Y. Ishii, K. Uemura, H. Kitazawa, T. Saito, and T. Itoh.
"Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from feces of the same human infant produce identical cyclic bacteriocin.", Food Microbiol. 18:407-415 (2001).
DOI=10.1006/fmic.2001.0412
Kawai, Y., Y. Ishii, K. Uemura, H. Kitazawa, T. Saito, and T. Itoh.
"Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from feces of the same human infant produce identical cyclic bacteriocin.", Food Microbiol. 18:407-415 (2001).
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Protein sequence
........10 ........20 ........30 ........40 ........50 ........60 | | | | | | IYWIADQFGI HLATGTARKL LDAMASGASL GTAFAAILGV TLPAWALAAA GALGATAA |
Wheel representation
Composition
Hydrophobicity
Composition
Formula | C261
H411
N67
O72
S1 |
Absent amino acids | CEN |
Common amino acids | A |
Mass (Da) | 5672.18 |
Net charge | +1 |
Isoelectric point | 7.54 |
Basic residues | 3 |
Acidic residues | 2 |
Hydrophobic residues | 35 |
Polar residues | 15 |
Aliphatic residues | 13 |
Tiny residues | 27 |
Boman Index | 47.31 |
Hydropathy Index | 0.997 |
Aliphatic Index | 31.62 |
Instability Index | 3.73 (stable) |
Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
Extinction Coefficient | 19490 M-1 cm-1 |
Absorbance 280nm | 219.12 |
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