Bacteriocin: michiganin-A
Accession: BAC163
Classification
Class: Lantibiotic
Genetics
Gene: micA
Producer and target organisms
Producer Organism: Clavibacter michiganensis subsp. michiganensis [Gram-positive]
Taxonomy: Bacteria
ActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter
Target organismsUnavailable data
Description
Lanthionine-containing peptide antibiotic (lantibiotic) active aganist S.michiganensis subspecies sepedonicus strain 2136 (MIC=30 pmol/ml). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor
Uniprot and PDB links
UniProt Entry: Q09T02
PDB EntryUnknown
[1]NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 48-68,FUNCTION, POST-TRANSLATIONAL MODIFICATIONS, AND MASS SPECTROMETRY.STRAIN=NCPPB 1468
PubMed=16957199;DOI=10.1128/AEM.00639-06
Holtsmark I., Mantzilas D., Eijsink V.G., Brurberg M.B.
"Purification, characterization, and gene sequence of michiganin A, anactagardine-like lantibiotic produced by the tomato pathogenClavibacter michiganensis subsp. michiganensis.", Appl. Environ. Microbiol. 72:5814-5821(2006)
PubMed=16957199;DOI=10.1128/AEM.00639-06
Holtsmark I., Mantzilas D., Eijsink V.G., Brurberg M.B.
"Purification, characterization, and gene sequence of michiganin A, anactagardine-like lantibiotic produced by the tomato pathogenClavibacter michiganensis subsp. michiganensis.", Appl. Environ. Microbiol. 72:5814-5821(2006)
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Gene structure
| Gene id | Name | Description | Location |
| BACGene757 | micA | michiganin A precursor lantibiotic precursor | 164..370 |
Protein sequence
| ........10 ........20 ........30 | | | SSSGWLCTLT IECGTIICAC R |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔21 | 21 | Lantibiotic michiganin-A. Feature identifier = PRO_5000141526. |
| Modified residue | 8 | 2,3-didehydrobutyrine (Probable). | |
| Cross-link | 2↔7 | 6 | Lanthionine (Ser-Cys) (Probable). |
| Cross-link | 10↔18 | 9 | Beta-methyllanthionine (Thr-Cys)(Probable). |
| Cross-link | 15↔20 | 6 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Composition
Hydrophobicity
Composition
| Formula | C92
H153
N25
O30
S4 |
| Absent amino acids | DFHKMNPQVY |
| Common amino acids | C |
| Mass (Da) | 2235.97 |
| Net charge | 0 |
| Isoelectric point | 6.02 |
| Basic residues | 1 |
| Acidic residues | 1 |
| Hydrophobic residues | 7 |
| Polar residues | 12 |
| Aliphatic residues | 5 |
| Tiny residues | 6 |
| Boman Index | -3.9 |
| Hydropathy Index | 0.89 |
| Aliphatic Index | 97.62 |
| Instability Index | 113.87 (unstable) |
| Half Life |
Mammalian : 1.9 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 5500 M-1 cm-1 |
| Absorbance 280nm | 287.5 |
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