Bacteriocin: michiganin-A

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Bacteriocin: michiganin-A

Accession: BAC163

Classification
Class: Lantibiotic
Genetics
Gene: micA
Producer and target organisms

Producer Organism: Clavibacter michiganensis subsp. michiganensis [Gram-positive]

Taxonomy: BacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrobacteriaceaeClavibacter

Target organismsUnavailable data

Description
Lanthionine-containing peptide antibiotic (lantibiotic) active aganist S.michiganensis subspecies sepedonicus strain 2136 (MIC=30 pmol/ml). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. The 62-67 beta-methyllanthionine thioether bond is oxidized to a sulfoxide. This is followed by membrane translocation and cleavage of the modified precursor
Uniprot and PDB links

UniProt Entry: Q09T02

PDB EntryUnknown

[1]NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 48-68,FUNCTION, POST-TRANSLATIONAL MODIFICATIONS, AND MASS SPECTROMETRY.STRAIN=NCPPB 1468
PubMed=16957199;DOI=10.1128/AEM.00639-06
Holtsmark I., Mantzilas D., Eijsink V.G., Brurberg M.B.
"Purification, characterization, and gene sequence of michiganin A, anactagardine-like lantibiotic produced by the tomato pathogenClavibacter michiganensis subsp. michiganensis.", Appl. Environ. Microbiol. 72:5814-5821(2006)
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Gene structure
genome of michiganin-A

Gene idNameDescriptionLocation
BACGene757micAmichiganin A precursor lantibiotic precursor164..370
micA
Protein sequence
 ........10 ........20 ........30 
          |          |          | 
 SSSGWLCTLT IECGTIICAC R

Wheel representation
wheel representation of michiganin-A
Protein sequence annotations
Features of michiganin-A

T
FeaturePosition(s)LengthDescription
PEPTIDE1↔2121
Lantibiotic michiganin-A.
Feature identifier = PRO_5000141526.
Modified residue82,3-didehydrobutyrine (Probable).
Cross-link2↔76Lanthionine (Ser-Cys) (Probable).
Cross-link10↔189Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link15↔206Beta-methyllanthionine (Thr-Cys)(Probable).
Composition
Hydrophobicity

Composition
Formula C92 H153 N25 O30 S4
Absent amino acids DFHKMNPQVY
Common amino acids C
Mass (Da) 2235.97
Net charge 0
Isoelectric point 6.02
Basic residues 1
Acidic residues 1
Hydrophobic residues 7
Polar residues 12
Aliphatic residues 5
Tiny residues 6
Boman Index -3.9
Hydropathy Index 0.89
Aliphatic Index 97.62
Instability Index 113.87 (unstable)
Half Life Mammalian : 1.9 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 5500 M-1 cm-1
Absorbance 280nm 287.5

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