Bacteriocin: Uberolysin

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Bacteriocin: Uberolysin

Accession: BAC162

Classification
Class: Unclassified
Genetics
Gene: ublA
Uniprot and PDB links

UniProt Entry: A5H1G9

PDB EntryUnknown

NUCLEOTIDE SEQUENCE
PubMed=17464077;DOI=10.1099/mic.0.2006/005967-0
Wirawan R.E., Swanson K.M., Kleffmann T., Jack R.W., Tagg J.R.
"Uberolysin: a novel cyclic bacteriocin produced by Streptococcus uberis.", Microbiology, 153(5):1619-30(2007).
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End Note
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Gene structure
genome of Uberolysin

Gene idNameDescriptionLocation
BACGene750PurH-like protein ORF3170..1330
BACGene751possible orphan response regulator ORF1; ComE-like; may be involved in regulation of ublAcomplement(1383..2138)
BACGene752ublAUblA circular bacteriocin uberolysin2438..2668
BACGene753ublBUblB putative maturation factor; putative protein involved in uberolysin maturation2758..4365
BACGene754ublCUblC integral membrane protein; possible accessory factor in uberolysin maturation4346..4891
BACGene755ublDUblD ABC-transporter; possible exporter of uberolysin4876..5532
BACGene756ublEUblE ORF2; possible immunity protein5529..5795
ublAublBublCublDublE
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 LAGYTGIASG TAKKVVDAID KGAAAFVIIS IISTVISAGA LGAVSASADF IILTVKNYIS RNLKAQAVIW

Wheel representation
wheel representation of Uberolysin
Protein sequence annotations
Features of Uberolysin


FeaturePosition(s)LengthDescription
PEPTIDE1↔7070
Bacteriocin uberolysin.
Feature identifier = PRO_0000326413.
Cross-link1↔7070Cyclopeptide (Leu-Trp).
Composition
Hydrophobicity

Composition
Formula C324 H534 N82 O93
Absent amino acids CEHMP
Common amino acids A
Mass (Da) 7085.54
Net charge +3
Isoelectric point 10.17
Basic residues 6
Acidic residues 3
Hydrophobic residues 39
Polar residues 21
Aliphatic residues 22
Tiny residues 27
Boman Index 19.34
Hydropathy Index 0.94
Aliphatic Index 132.57
Instability Index -2.28 (stable)
Half Life Mammalian : 5.5 hour
Yeast : 3 min
E. coli : 2 min
Extinction Coefficient 8480 M-1 cm-1
Absorbance 280nm 122.9

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