Bacteriocin: Thermophilin 1277

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Bacteriocin: Thermophilin 1277

Accession: BAC155

Classification

Class: Lantibiotic

Genetics

Gene: tepA

Producer and target organisms

Producer Organism: Streptococcus thermophilus [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsStreptomyces thermophilus SBT10137 - Streptomyces thermophilus SBT10814 - Streptomyces thermophilus ATCC19258 - Streptomyces thermophilus SBT10813 - Streptomyces thermophilus SBT10838 - Streptomyces thermophilus SBT10914 - Lactobacillus acidophilus JCM1132 - Lactobacillus delbrueckii subsp. bulgaricus SBT0164 - Lactobacillus helveticus SBT10511 - Lactobacillus helveticus SBT2171 - Lactobacillus helveticus SBT1270 - Lactococcus lactis subsp. lactis ATCC11454 - Microbacterium lacticum SBT3513 - Microbacterium lacticum BT3514 - Enterococcus fecalis BT3515 - Clostridium butylicum SBT3516 - Clostridium sprogenes IFH 493 - Clostridium tyrobutylicum SBT3517 - Bacillus cereus IFO13494 -

Note: not active against Lactobacillus gasseri SBT2055 - Lactobacillus gasseri JCM1131 - Staphylococcus aureus ATCC25923 - Listeria innocua IFH178 - Listeria innocua IFH180 - Listeria monocytogenes IFH181 - Listeria monocytogenes IFH182 - Listeria monocytogenes IFH179

Description

First report on a lantibiotic produced by Strep. thermophilus strain. The DNA sequence of this region is identical to bovicin HJ50 produced by Strep. bovis HJ50, which belongs to the same genus to Strep. thermophilus.

Related Entries

thermophilin A thermophilin 13

Uniprot and PDB links

UniProt Entry: A7M695

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

characterization of thermophilin 1277
PubMed=17381740;DOI=10.1111/j.1365-2672.2006.03159.x
Kabuki T., Uenishi H., Watanabe M., Seto Y., Nakajima H.
"Characterization of a bacteriocin, Thermophilin 1277, produced by Streptococcus thermophilus SBT1277.", J. Appl. Microbiol. 102(4):971-980(2007)

[Ref. 2] | View abstract | Export citation

NUCLEOTIDE SEQUENCE of thermophilin 1277
PubMed=19191960;
Kabuki T., Uenishi H., Seto Y., Yoshioka T., Nakajima H.
"A unique lantibiotic, thermophilin 1277, containing a disulfide bridge and two thioether bridges.", J. Appl. Microbiol. 106(3):853-862(2009)

Gene structure

Gene id	Name	Description	Location
BACGene693		hypothetical protein	1..1050
BACGene694		hypothetical protein	1120..1419
BACGene695		hypothetical protein	1432..1722
BACGene696	tepA	thermophilin 1277	1861..2037
BACGene697	tepM	hypothetical bacteriocin modified enzyme	2118..4631
BACGene698	tepT	hypothetical bacteriocin transporter	4645..6687
BACGene699	tepF	hypothetical bacteriocin immunity protein	6662..7570
BACGene700	tepF	hypothetical protein	7554..8276
BACGene701	tepF	hypothetical protein	8283..8999
BACGene702	tepK	hypothetical bacteriocin immunity protein	8989..10500
BACGene703	tepR	hypothetical bacteriocin immunity protein	10721..11074
BACGene704	tepR	hypothetical protein	11143..11301
BACGene705	tepR	hypothetical protein	11313..11798
BACGene706	tepR	hypothetical protein	12012..12662
BACGene707	tepR	hypothetical protein	12702..13790
BACGene708	tepR	hypothetical protein	13844..14071
BACGene709	tepR	hypothetical protein	14075..14470
BACGene710	tepR	hypothetical protein	complement(14831..15130)
BACGene711	tepR	hypothetical protein	complement(15117..15485)
BACGene712	tepR	hypothetical protein	complement(15482..16402)

Protein sequence

........10 ........20 ........30 ........40
| | | |
ADRGWIKTLT KDCPNVISSI CAGTIITACK NCA

Wheel representation

Composition

Hydrophobicity

Composition

Formula	C₁₄₇ H₂₄₈ N₄₂ O₄₆ S₄
Absent amino acids	EFHMQY
Common amino acids	I
Mass (Da)	3486.66
Net charge	+2
Isoelectric point	8.34
Basic residues	4
Acidic residues	2
Hydrophobic residues	12
Polar residues	14
Aliphatic residues	7
Tiny residues	8
Boman Index	-29.24
Hydropathy Index	0.3
Aliphatic Index	91.82
Instability Index	24.98 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	5500 M^-1 cm^-1
Absorbance 280nm	179.69

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