Bacteriocin: Bacteriocin E50-52
Accession: BAC153
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Enterococcus faecium (Streptococcus faecium) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesEnterococcaceaeEnterococcus
Target organismsCampylobacter jejuni (n=98; MIC=0.025-6.4 μg/mL) - Yersinia enterocolitica (n=24) - Yersinia pseudotuberculosis (n=2) - Staphylococcus aureus (n=26; MIC=0.2-0.8μg/mL) - Staphylococcus epidermidis (n=4; MIC=0.2-0.8μg/mL) - Listeria monocytogenes (n=10; MIC=2-8 μg/mL) - Listeria grayi - Listeria innocua - Listeria ivanovii - Listeria Listeria denitrificans (MIC=32 μg/mL) - Escherichia coli O157:H7 (MIC<1.6 μg/mL) - Morganella morganii - Salmonella spp. (n=4) - Shigella dysenteriae
Description
Bacteriocin active against the Gram-negative bacteria C.jejuni, Y.enterocolitica and Y.pseudotuberculosis, and the Gram-positive bacteria S.aureus, S.epidermidis, Listeria monocytogenes and Listeria spp. When added to the drinking water of chickens, causes a decrease in the levels of C.jejuni and S.enteritidis in the ceca, and in the levels of S.enteritidis in the liver and spleen
Uniprot and PDB links
UniProt Entry: P85148
PDB EntryUnknown
PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASSSPECTROMETRY.STRAIN=50-52
PubMed=18293921;DOI=10.1021/jf073284g
Svetoch E.A., Eruslanov B.V., Perelygin V.V., Mitsevich E.V., Mitsevich I.P., Borzenkov V.N., Levchuk V.P., Svetoch O.E., Kovalev Y.N., Stepanshin Y.G., Siragusa G.R., Seal B.S., Stern N.J.
"Diverse antimicrobial killing by Enterococcus faecium E 50-52bacteriocin.", J. Agric. Food Chem. 56:1942-1948(2008)
PubMed=18293921;DOI=10.1021/jf073284g
Svetoch E.A., Eruslanov B.V., Perelygin V.V., Mitsevich E.V., Mitsevich I.P., Borzenkov V.N., Levchuk V.P., Svetoch O.E., Kovalev Y.N., Stepanshin Y.G., Siragusa G.R., Seal B.S., Stern N.J.
"Diverse antimicrobial killing by Enterococcus faecium E 50-52bacteriocin.", J. Agric. Food Chem. 56:1942-1948(2008)
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | TTKNYGNGVC NSVNWCQCGN VWASCNLATG CAAWLCKLA |
Wheel representation
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| CHAIN | 1↔39 | 39 | Bacteriocin E50-52. Feature identifier = PRO_0000341451. |
Composition
Hydrophobicity
Composition
| Formula | C175
H267
N51
O53
S6 |
| Absent amino acids | DEFHIMPR |
| Common amino acids | CN |
| Mass (Da) | 4144.4 |
| Net charge | +2 |
| Isoelectric point | 8.11 |
| Basic residues | 2 |
| Acidic residues | 0 |
| Hydrophobic residues | 14 |
| Polar residues | 22 |
| Aliphatic residues | 6 |
| Tiny residues | 11 |
| Boman Index | -16.77 |
| Hydropathy Index | 0.16 |
| Aliphatic Index | 65.13 |
| Instability Index | -3.85 (stable) |
| Half Life |
Mammalian : 7.2 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 17990 M-1 cm-1 |
| Absorbance 280nm | 483.29 |
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