Bacteriocin: Enterocin 96

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Bacteriocin: Enterocin 96

Accession: BAC149

Classification
Class: Class II
Genetics
Gene: Unidentified
Description
search in protein databases revealed that enterocin 96 was identical to the C-terminal part of a putative uncharacterized protein from Enterococcus faecalis V583 (accession number EF_B0056) identified after complete genome sequencing of this strain. The amino acid sequence of this protein contains an N-terminal extension of 26 amino acids with two C-terminal 1 glycyl residues. The likely presence of such an extension for enterocin 96 was investigated by sequencing its structural gene.

PubMed=19411428;DOI=10.1128/AEM.02772-08
Izquierdo, Esther, Wagner, Camille, Marchioni, Eric, Aoude-Werner, Dalal, Ennahar, Said.
"Enterocin 96, a novel class II bacteriocin produced by Enterococcus faecalis WHE 96, isolated from Munster cheese", Appl. Environ. Microbiol. 2009 0: AEM.02772-08.
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Protein sequence
 ........10 ........20 ........30 ........40 ........50 
          |          |          |          |          | 
 MSKRDCNLMK ACCAGQAVTY AIHSLLNRLG GDSSDPAGCN DIVRKYCK

Wheel representation
wheel representation of Enterocin 96
Composition
Hydrophobicity

Composition
Formula C214 H355 N67 O68 S7
Absent amino acids EFW
Common amino acids AC
Mass (Da) 5493.92
Net charge +4
Isoelectric point 8.5
Basic residues 8
Acidic residues 4
Hydrophobic residues 13
Polar residues 19
Aliphatic residues 8
Tiny residues 13
Boman Index -86.9
Hydropathy Index -0.25
Aliphatic Index 71.25
Instability Index 53.47 (unstable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 2980 M-1 cm-1
Absorbance 280nm 68.72

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