Bacteriocin: Carnocyclin-A
Accession: BAC148
Producer Organism: Carnobacterium maltaromaticum (Carnobacterium piscicola) [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesCarnobacteriaceaeCarnobacterium
Target organismsGram-positive: Carnobacterium maltaromaticum UAL26 - Carnobacterium maltaromaticum LV17A - Carnobacterium divergens LV13 - Brochothrix campestris ATCC 43754 - Brochothrix thermosphacta ATCC 11509 - Enterococcus faecalis ATCC 7080 - Enterococcus faecium ATCC 19434 - Enterococcus faecium BFE900 - Lactococcus lactis subsp. lactis ATCC 11454 - Lactococcus lactis subsp. cremoris ATCC 11602 - Lactococcus lactis subsp. lactis DPC3147 - Lactobacillus sakei 706 - Lactobacillus sakei UAL1218 - Leuconostoc mesenteroides Y105 - Pediococcus acidilactici PAC1.0 - Listeria monocytogenes ATCC 15313 - Listeria monocytogenes H7762 - Listeria monocytogenes ATCC 43256 - Listeria monocytogenes HPB 642 - Listeria monocytogenes UAFM 1 - Listeria monocytogenes UAFM 15 - Staphylococcus aureus ATCC 25923 - Staphylococcus aureus ATCC 6538 - Staphylococcus aureus ATCC 29213 -
Note: No activity was observed against the gram-negative strains that were tested.
UniProt Entry: B2MVM5
PDB Entry2KJF resolved by NMR
PubMed=18552180;DOI=10.1128/AEM.00817-08
Martin-Visscher L.A., van Belkum M.J., Garneau-Tsodikova S., Whittal R.M., Zheng J., McMullen L.M., Vederas J.C.;
"Isolation and characterization of carnocyclin a, a novel circularbacteriocin produced by Carnobacterium maltaromaticum UAL307.", Appl. Environ. Microbiol. 74:4756-4763 (2008).
| Gene id | Name | Description | Location |
| BACGene665 | hypothetical protein Orf1 | 83..262 | |
| BACGene666 | hypothetical protein Orf2 | 570..821 | |
| BACGene667 | cclB | CclB | 1223..2674 |
| BACGene668 | cclI | CclI immunity protein | 2747..2929 |
| BACGene669 | cclT | CclT ATP-binding protein | 2958..3584 |
| BACGene670 | cclC | CclC DUF95 family of membrane proteins | 3562..4140 |
| BACGene671 | cclD | CclD | 4239..4721 |
| BACGene672 | cclA | carnocyclin A precursor circular bacteriocin; anti-bacterial peptide | 4780..4974 |
| BACGene673 | cclE | CclE | 5402..5854 |
| BACGene674 | cclF | CclF putative HlyD-type family of accessory proteins | 5885..7087 |
| BACGene675 | cclG | CclG ATP-binding protein | 7084..7767 |
| BACGene676 | cclH | CclH putative ABC transporter permease | 7764..8960 |
| BACGene677 | cclH | hypothetical protein Orf3 | 9197..9319 |
| BACGene678 | cclH | hypothetical protein Orf4 | complement(10211..10642) |
| BACGene679 | trxA | TrxA TRX family of thioredoxin proteins | 11267..11698 |
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 | | | | | | | LVAYGIAQGT AEKVVSLINA GLTVGSIISI LGGVTVGLSG VFTAVKAAIA KQGIKKAIQL |
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔60 | 60 | Carnocyclin-A. Feature identifier = PRO_0000361697. |
| Cross-link | 1↔60 | 60 | Cyclopeptide (Leu-Leu). |
| CONFLICT | 28 | I -> L (in Ref. 1; AA sequence). | |
| TURN | 2↔5 | 4 | |
| HELIX | 8↔18 | 11 | |
| TURN | 19↔21 | 3 | |
| HELIX | 24↔31 | 8 | |
| TURN | 36↔38 | 3 | |
| HELIX | 39↔51 | 13 | |
| HELIX | 54↔59 | 6 |
| Formula | C269
H463
N69
O76
|
| Absent amino acids | CDHMPRW |
| Common amino acids | AGIV |
| Mass (Da) | 5862 |
| Net charge | +4 |
| Isoelectric point | 10.7 |
| Basic residues | 5 |
| Acidic residues | 1 |
| Hydrophobic residues | 32 |
| Polar residues | 19 |
| Aliphatic residues | 22 |
| Tiny residues | 22 |
| Boman Index | 47.09 |
| Hydropathy Index | 1.06 |
| Aliphatic Index | 144.67 |
| Instability Index | 5.83 (stable) |
| Half Life |
Mammalian : 5.5 hour Yeast : 3 min E. coli : 2 min |
| Extinction Coefficient | 1490 M-1 cm-1 |
| Absorbance 280nm | 25.25 |
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