Bacteriocin: nisin U
Accession: BAC147
Producer Organism: Streptococcus uberis ATCC 27958 [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Streptococcaceae
Streptococcus
Target organismsActive against Streptococcus pyogenes (10/11) - Streptococcus uberis (20/26) - Streptococcus agalactiae (2/4) - Streptococcus dysgalactiae (3/4) - Staphylococcus simulans (1/1) - Staphylococcus cohnii (1/1) - Lactococcus lactis (1/3) - Lactobacillus acidophilus (1/1) - Streptococcus mitis (2/2) -
NOTE: not active against Lactobacillus casei (0/1) - Streptococcus rattus (0/2) - Enterococcus faecalis (0/3) - Staphylococcus aureus (0/2) - Streptococcus salivarius (0/7) - Streptococcus mutans (0/7) - Escherichia coli (0/4) - Enterobacter aerogenes (0/1) - Salmonella enterica serovar Typhimurium (0/1)
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
UniProt Entry: Q2QBT0
PDB EntryUnknown
PubMed=16461661;DOI=10.1128/AEM.72.2.1148-1156.2006
Wirawan R.E., Klesse N.A., Jack R.W., Tagg J.R.
"Molecular and genetic characterization of a novel nisin variant produced by Streptococcus uberis.", Appl. Environ. Microbiol. 72:1148-1156(2006).
Gene id | Name | Description | Location |
BACGene654 | nsuP | NsuP nisin U leader peptidase | 594..1979 |
BACGene655 | nsuR | NsuR response regulator | 2007..2705 |
BACGene656 | nsuK | NsuK histidine kinase | 2686..4032 |
BACGene657 | nsuF | NsuF | 4124..4816 |
BACGene658 | nsuE | NsuE | 4803..5564 |
BACGene659 | nsuG | NsuG | 5533..6225 |
BACGene660 | nsuA | NsuA nisin U lantibiotic | 6968..7135 |
BACGene661 | nsuB | NsuB lantibiotic biosynthesis protein | 7381..10356 |
BACGene662 | nsuT | NsuT ABC transporter | 10349..12145 |
BACGene663 | nsuC | NsuC | 12138..13415 |
BACGene664 | nsuI | NsuI putative immunity peptide | 13417..14133 |
........10 ........20 ........30 ........40 | | | | ITSKSLCTPG CKTGILMTCP LKTATCGCHF G |
Feature | Position(s) | Length | Description |
PEPTIDE | 1↔31 | 31 | Lantibiotic nisin-U. Feature identifier = PRO_0000326411.
|
Modified residue | 2 | 2,3-didehydrobutyrine (Probable). | |
Modified residue | 5 | 2,3-didehydroalanine (Ser) (Probable). | |
Modified residue | 18 | 2,3-didehydrobutyrine (Probable). | |
Cross-link | 3↔7 | 5 | Lanthionine (Ser-Cys) (Probable). |
Cross-link | 8↔11 | 4 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Cross-link | 13↔19 | 7 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Cross-link | 23↔26 | 4 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Cross-link | 25↔28 | 4 | Beta-methyllanthionine (Thr-Cys)(Probable). |
Formula | C134
H226
N36
O40
S6 |
Absent amino acids | DENQRVWY |
Common amino acids | T |
Mass (Da) | 3192.37 |
Net charge | +4 |
Isoelectric point | 8.51 |
Basic residues | 4 |
Acidic residues | 0 |
Hydrophobic residues | 7 |
Polar residues | 17 |
Aliphatic residues | 5 |
Tiny residues | 7 |
Boman Index | -1.63 |
Hydropathy Index | 45 |
Aliphatic Index | 66.13 |
Instability Index | 30.65 (stable) |
Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
Extinction Coefficient | 0 M-1 cm-1 |
Absorbance 280nm | 8.33 |
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