Bacteriocin: nisin U

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Bacteriocin: nisin U

Accession: BAC147

Classification
Class: Lantibiotic
Genetics
Gene: nsuA
Producer and target organisms

Producer Organism: Streptococcus uberis ATCC 27958 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsActive against Streptococcus pyogenes (10/11) - Streptococcus uberis (20/26) - Streptococcus agalactiae (2/4) - Streptococcus dysgalactiae (3/4) - Staphylococcus simulans (1/1) - Staphylococcus cohnii (1/1) - Lactococcus lactis (1/3) - Lactobacillus acidophilus (1/1) - Streptococcus mitis (2/2) -

NOTE: not active against Lactobacillus casei (0/1) - Streptococcus rattus (0/2) - Enterococcus faecalis (0/3) - Staphylococcus aureus (0/2) - Streptococcus salivarius (0/7) - Streptococcus mutans (0/7) - Escherichia coli (0/4) - Enterobacter aerogenes (0/1) - Salmonella enterica serovar Typhimurium (0/1)

Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Uniprot and PDB links

UniProt Entry: Q2QBT0

PDB EntryUnknown

NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-44, POST-TRANSLATIONAL MODIFICATIONS, FUNCTION, AND MASS SPECTROMETRY, STRAIN=42.
PubMed=16461661;DOI=10.1128/AEM.72.2.1148-1156.2006
Wirawan R.E., Klesse N.A., Jack R.W., Tagg J.R.
"Molecular and genetic characterization of a novel nisin variant produced by Streptococcus uberis.", Appl. Environ. Microbiol. 72:1148-1156(2006).
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Gene structure
genome of nisin U

Gene idNameDescriptionLocation
BACGene654nsuPNsuP nisin U leader peptidase594..1979
BACGene655nsuRNsuR response regulator2007..2705
BACGene656nsuKNsuK histidine kinase2686..4032
BACGene657nsuFNsuF4124..4816
BACGene658nsuENsuE4803..5564
BACGene659nsuGNsuG5533..6225
BACGene660nsuANsuA nisin U lantibiotic6968..7135
BACGene661nsuBNsuB lantibiotic biosynthesis protein7381..10356
BACGene662nsuTNsuT ABC transporter10349..12145
BACGene663nsuCNsuC12138..13415
BACGene664nsuINsuI putative immunity peptide13417..14133
nsuPnsuRnsuKnsuFnsuEnsuGnsuAnsuBnsuTnsuCnsuI
Protein sequence
 ........10 ........20 ........30 ........40 
          |          |          |          | 
 ITSKSLCTPG CKTGILMTCP LKTATCGCHF G

Wheel representation
wheel representation of nisin U
Protein sequence annotations
Features of nisin U

TST
FeaturePosition(s)LengthDescription
PEPTIDE1↔3131
Lantibiotic nisin-U.
Feature identifier = PRO_0000326411.
Modified residue22,3-didehydrobutyrine (Probable).
Modified residue52,3-didehydroalanine (Ser) (Probable).
Modified residue182,3-didehydrobutyrine (Probable).
Cross-link3↔75Lanthionine (Ser-Cys) (Probable).
Cross-link8↔114Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link13↔197Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link23↔264Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link25↔284Beta-methyllanthionine (Thr-Cys)(Probable).
Composition
Hydrophobicity

Composition
Formula C134 H226 N36 O40 S6
Absent amino acids DENQRVWY
Common amino acids T
Mass (Da) 3192.37
Net charge +4
Isoelectric point 8.51
Basic residues 4
Acidic residues 0
Hydrophobic residues 7
Polar residues 17
Aliphatic residues 5
Tiny residues 7
Boman Index -1.63
Hydropathy Index 45
Aliphatic Index 66.13
Instability Index 30.65 (stable)
Half Life Mammalian : 20 hour
Yeast : 30 min
E. coli : >10 hour
Extinction Coefficient 0 M-1 cm-1
Absorbance 280nm 8.33

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