Bacteriocin: nisin U

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Bacteriocin: nisin U

Accession: BAC147

Classification

Class: Lantibiotic

Genetics

Gene: nsuA

Producer and target organisms

Producer Organism: Streptococcus uberis ATCC 27958 [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesStreptococcaceaeStreptococcus

Target organismsActive against Streptococcus pyogenes (10/11) - Streptococcus uberis (20/26) - Streptococcus agalactiae (2/4) - Streptococcus dysgalactiae (3/4) - Staphylococcus simulans (1/1) - Staphylococcus cohnii (1/1) - Lactococcus lactis (1/3) - Lactobacillus acidophilus (1/1) - Streptococcus mitis (2/2) -

NOTE: not active against Lactobacillus casei (0/1) - Streptococcus rattus (0/2) - Enterococcus faecalis (0/3) - Staphylococcus aureus (0/2) - Streptococcus salivarius (0/7) - Streptococcus mutans (0/7) - Escherichia coli (0/4) - Enterobacter aerogenes (0/1) - Salmonella enterica serovar Typhimurium (0/1)

Description

Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.

Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.

Related Entries

Nisin A Nisin Z Nisin Q Nisin F

Uniprot and PDB links

UniProt Entry: Q2QBT0

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 25-44, POST-TRANSLATIONAL MODIFICATIONS, FUNCTION, AND MASS SPECTROMETRY, STRAIN=42.
PubMed=16461661;DOI=10.1128/AEM.72.2.1148-1156.2006
Wirawan R.E., Klesse N.A., Jack R.W., Tagg J.R.
"Molecular and genetic characterization of a novel nisin variant produced by Streptococcus uberis.", Appl. Environ. Microbiol. 72:1148-1156(2006).

Gene structure

Gene id	Name	Description	Location
BACGene654	nsuP	NsuP nisin U leader peptidase	594..1979
BACGene655	nsuR	NsuR response regulator	2007..2705
BACGene656	nsuK	NsuK histidine kinase	2686..4032
BACGene657	nsuF	NsuF	4124..4816
BACGene658	nsuE	NsuE	4803..5564
BACGene659	nsuG	NsuG	5533..6225
BACGene660	nsuA	NsuA nisin U lantibiotic	6968..7135
BACGene661	nsuB	NsuB lantibiotic biosynthesis protein	7381..10356
BACGene662	nsuT	NsuT ABC transporter	10349..12145
BACGene663	nsuC	NsuC	12138..13415
BACGene664	nsuI	NsuI putative immunity peptide	13417..14133

Protein sequence

........10 ........20 ........30 ........40
| | | |
ITSKSLCTPG CKTGILMTCP LKTATCGCHF G

Wheel representation

Protein sequence annotations

Feature	Position(s)	Length	Description
PEPTIDE	1↔31	31	Lantibiotic nisin-U. Feature identifier = PRO_0000326411.
Modified residue	2		2,3-didehydrobutyrine (Probable).
Modified residue	5		2,3-didehydroalanine (Ser) (Probable).
Modified residue	18		2,3-didehydrobutyrine (Probable).
Cross-link	3↔7	5	Lanthionine (Ser-Cys) (Probable).
Cross-link	8↔11	4	Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link	13↔19	7	Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link	23↔26	4	Beta-methyllanthionine (Thr-Cys)(Probable).
Cross-link	25↔28	4	Beta-methyllanthionine (Thr-Cys)(Probable).

Composition

Hydrophobicity

Composition

Formula	C₁₃₄ H₂₂₆ N₃₆ O₄₀ S₆
Absent amino acids	DENQRVWY
Common amino acids	T
Mass (Da)	3192.37
Net charge	+4
Isoelectric point	8.51
Basic residues	4
Acidic residues	0
Hydrophobic residues	7
Polar residues	17
Aliphatic residues	5
Tiny residues	7
Boman Index	-1.63
Hydropathy Index	45
Aliphatic Index	66.13
Instability Index	30.65 (stable)
Half Life	Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour
Extinction Coefficient	0 M^-1 cm^-1
Absorbance 280nm	8.33

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