Bacteriocin: Nisin Q
Accession: BAC145
Classification
Class: Lantibiotic
Genetics
Gene: nisQ
Producer and target organisms
Producer Organism: Lactococcus lactis [Gram-positive]
Taxonomy: Bacteria
FirmicutesLactobacillalesStreptococcaceaeLactococcus
Target organismswide range of Gram-positive bacteria including LAB - Bacillus sp - Listeria sp - Micrococcus sp -
Note:The antibacterial spectrum is similar to that of nisins A & Z(not completely identical)
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Uniprot and PDB links
UniProt Entry: Q76HP8
PDB EntryUnknown
NUCLEOTIDE SEQUENCE, STRAIN=61-14
MEDLINE=22793497;PubMed=12913315;DOI=10.1271/bbb.67.1616
Zendo T., Fukao M., Ueda K., Higuchi T., Nakayama J., Sonomoto K.
"Identification of the lantibiotic nisin Q, a new natural nisinvariant produced by Lactococcus lactis 61-14 isolated from a river in Japan.", Biosci. Biotechnol. Biochem. 67:1616-1619 (2003).
MEDLINE=22793497;PubMed=12913315;DOI=10.1271/bbb.67.1616
Zendo T., Fukao M., Ueda K., Higuchi T., Nakayama J., Sonomoto K.
"Identification of the lantibiotic nisin Q, a new natural nisinvariant produced by Lactococcus lactis 61-14 isolated from a river in Japan.", Biosci. Biotechnol. Biochem. 67:1616-1619 (2003).
NUCLEOTIDE SEQUENCE, STRAIN=61-14
PubMed=19120645;DOI=10.1111/j.1365-2672.2008.03958.x
Yoneyama F., Fukao M., Zendo T., Nakayama J., Sonomoto K.
"Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14.", J. Appl. Microbiol. 105:1982-1990 (2008).
PubMed=19120645;DOI=10.1111/j.1365-2672.2008.03958.x
Yoneyama F., Fukao M., Zendo T., Nakayama J., Sonomoto K.
"Biosynthetic characterization and biochemical features of the third natural nisin variant, nisin Q, produced by Lactococcus lactis 61-14.", J. Appl. Microbiol. 105:1982-1990 (2008).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene642 | nisQ | nisin Q precursor | 173..346 |
| BACGene643 | niqB | lantibiotic dehydratase | 453..3434 |
| BACGene644 | niqT | ATP-binding cassette transporter | 3445..5247 |
| BACGene645 | niqC | lantibiotic cyclase | 5240..6481 |
| BACGene646 | niqI | immunity protein self-protection | 6478..7215 |
| BACGene647 | niqP | leader peptidase | 7217..9304 |
| BACGene648 | niqR | response regulator | 9370..10056 |
| BACGene649 | niqK | histidine kinase | 10049..11392 |
| BACGene650 | niqF | immunity protein self-protection | 11490..12167 |
| BACGene651 | niqE | immunity protein self-protection | 12169..12897 |
| BACGene652 | niqG | immunity protein self-protection | 12884..13528 |
Protein sequence
| ........10 ........20 ........30 ........40 | | | | ITSISLCTPG CKTGVLMGCN LKTATCNCSV HVSK |
Wheel representation
Composition
Hydrophobicity
Composition
| Formula | C143
H249
N41
O46
S6 |
| Absent amino acids | DEFQRWY |
| Common amino acids | CT |
| Mass (Da) | 3489.73 |
| Net charge | +4 |
| Isoelectric point | 8.51 |
| Basic residues | 4 |
| Acidic residues | 0 |
| Hydrophobic residues | 9 |
| Polar residues | 19 |
| Aliphatic residues | 8 |
| Tiny residues | 8 |
| Boman Index | -10.94 |
| Hydropathy Index | 0.52 |
| Aliphatic Index | 85.88 |
| Instability Index | 13.45 (stable) |
| Half Life |
Mammalian : 20 hour Yeast : 30 min E. coli : >10 hour |
| Extinction Coefficient | 0 M-1 cm-1 |
| Absorbance 280nm | 7.68 |
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