Bacteriocin: Epilancin 15x
Accession: BAC144
Classification
Class: Unclassified
Genetics
Gene: Unidentified
Producer and target organisms
Producer Organism: Staphylococcus Epidermidis [Gram-positive]
Taxonomy: Bacteria
FirmicutesBacillalesStaphylococcus.
Target organismsUnavailable data
Description
Mode of action:
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Structure:
Lanthionine Linkage Between 12 And 16 Methyl-Lanthionine Linkages Between 20 And 23, And 22 And 25, Respectively.
Lanthionine-containing peptide antibiotic (lantibiotic). The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
Post-translational modification:
Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor. plasmid DNA. The sequences reported are absolutely identical.
Structure:
Lanthionine Linkage Between 12 And 16 Methyl-Lanthionine Linkages Between 20 And 23, And 22 And 25, Respectively.
Uniprot and PDB links
UniProt Entry: P86047
PDB Entry1W9N resolved by NMR.
PROTEIN SEQUENCE AND STRUCTURE, STRAIN=15x150.
PubMed=15792796;DOI=10.1016/j.febslet.2005.01.083
M.Ekkelenkamp, M.G.M.Hanssen, S.-T.D.Hsu, A.De Jong, D.Milatovic, J.Verhoef, N.A.J.Van Nuland.
"Isolation And Characterization Of Epilancin 15x, A Novel Antibiotic From A Clinical Strain Of Staphylococcus Epidermidis", FEBS Letters(2005) 579(9):1917-1922.
PubMed=15792796;DOI=10.1016/j.febslet.2005.01.083
M.Ekkelenkamp, M.G.M.Hanssen, S.-T.D.Hsu, A.De Jong, D.Milatovic, J.Verhoef, N.A.J.Van Nuland.
"Isolation And Characterization Of Epilancin 15x, A Novel Antibiotic From A Clinical Strain Of Staphylococcus Epidermidis", FEBS Letters(2005) 579(9):1917-1922.
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Protein sequence
| ........10 ........20 ........30 ........40 | | | | XAAIVKAAIK AAKKLCRGFA LACGCHFAGK K |
Warning: contain modified amino acids (2OP, DHA, DBU, ABA)
Wheel representation
3D Structure
Protein sequence annotations
| Feature | Position(s) | Length | Description |
| PEPTIDE | 1↔31 | 31 | Lantibiotic epilancin 15X. Feature identifier = PRO_0000379930. |
| Modified residue | 1 | Lactic acid. | |
| Modified residue | 3 | 2,3-didehydroalanine (Ser). | |
| Modified residue | 7 | 2,3-didehydrobutyrine. | |
| Modified residue | 8 | 2,3-didehydrobutyrine. | |
| Modified residue | 28 | 2,3-didehydrobutyrine. | |
| Cross-link | 12↔16 | 5 | Lanthionine (Ser-Cys). |
| Cross-link | 20↔23 | 4 | Beta-methyllanthionine (Thr-Cys). |
| Cross-link | 22↔25 | 4 | Beta-methyllanthionine (Thr-Cys). |
Composition
Hydrophobicity
Composition
| Formula | C137
H231
N41
O30
S3 |
| Absent amino acids | DEMNPQSTWY |
| Common amino acids | A |
| Mass (Da) | 3157.34 |
| Net charge | +8 |
| Isoelectric point | 10.8 |
| Basic residues | 8 |
| Acidic residues | 0 |
| Hydrophobic residues | 16 |
| Polar residues | 6 |
| Aliphatic residues | 5 |
| Tiny residues | 12 |
| Boman Index | -0.25 |
| Hydropathy Index | 0.57 |
| Aliphatic Index | 88.71 |
| Instability Index | 16.08 (stable) |
| Half Life |
Mammalian : 0 Yeast : 0 E. coli : 0 |
| Extinction Coefficient | 0 M-1 cm-1 |
| Absorbance 280nm | 4.17 |
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