Bacteriocin: Enterocin AS-48 (BACTERIOCIN AS-48)
Accession: BAC143
Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]
Taxonomy: BacteriaFirmicutes
Lactobacillales
Enterococcaceae
Enterococcus.
Target organismsGram-positive species: - Bacillus subtilus - Bacillus cereus - Bacillus circulans - Bacillus megaterium - Corynebacterium glutamicum - Corynebacterium bovis - Mycobacterium phlei - Nocardia corrallina - Micrococcus luteus - Micrococcus lysodeikticus - Staphylococcus aureus - Streptococcus faecalis - Streptococcus faecium -
Gram-negative species: - Enterobacter cloacae - Escherichia coli - Klebsiella pneumoniae - Proteus inconstans - Salmonella typhimurium - Shigella sonnei - Pseudomonas fluorescens - Pseudomonas aeruginosa - Pseudomonas reptilivora -
note: not active against Alcaligenes faecalis - Proteus sp - Pasteurella sp
UniProt Entry: Q47765
PDB Entry1E68 resolved by NMR 1O82 resolved by X-ray 1O83 resolved by X-ray 1O84 resolved by X-ray 4RGD resolved by X-ray
MEDLINE=95014079;PubMed=7929005;
Martinez-Bueno M., Maqueda M., Galvez A., Samyn B., Van Beeumen J., Coyette J., Valdivia E.
"Determination of the gene sequence and the molecular structure of the enterococcal peptide antibiotic AS-48.", J. Bacteriol. 176:6334-6339(1994).
MEDLINE=95010683;PubMed=7925951;DOI=10.1016/0014-5793(94)00925-2
Samyn B., Martinez-Bueno M., Devreese B., Maqueda M., Galvez A., Valdivia E., Coyette J., Van Beeumen J.
"The cyclic structure of the enterococcal peptide antibiotic AS-48.", FEBS Lett. 352:87-90(1994).
PubMed=3098396;
Gálvez A., Maqueda M., Valdivia E., Quesada A., Montoya E.
"Characterization and partial purification of a broad spectrum antibiotic AS-48 produced by Streptococcus faecalis.", Can. j. Microbiol. 32(10):765-771(1986)
Gene id | Name | Description | Location |
BACGene634 | as-48 | AS-48 protein | 70..387 |
BACGene635 | as-48B | AS-48B protein AS-48 maturation and biosynthesis | 461..2152 |
BACGene636 | as-48C | AS-48C protein putative accessory factor in AS-48 secretion | 2133..2669 |
BACGene637 | as-48C1 | AS-48C1 protein putative ABC transporter | 3041..3535 |
BACGene638 | as-48D | AS-48D protein putative ABC transporter | 3535..4194 |
BACGene639 | as-48D1 | AS-48D1 protein as-48 immunity gene | 4181..4351 |
BACGene640 | as-48D1 | hypothetical protein ORF6 | 4556..5065 |
BACGene641 | as-48D1 | hypothetical protein ORF7 | 5043..6266 |
........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | MAKEFGIPAA VAGTVLNVVE AGGWVTTIVS ILTAVGSGGL SLLAAAGRES IKAYLKKEIK KKGKRAVIAW |
Feature | Position(s) | Length | Description |
CHAIN | 1↔70 | 70 | peptide antibiotic AS-48. Feature identifier = PRO_5000146287.
|
Formula | C328
H549
N87
O89
S1 |
Absent amino acids | CDHQ |
Common amino acids | A |
Mass (Da) | 7186.77 |
Net charge | +6 |
Isoelectric point | 10.83 |
Basic residues | 10 |
Acidic residues | 4 |
Hydrophobic residues | 35 |
Polar residues | 19 |
Aliphatic residues | 20 |
Tiny residues | 25 |
Boman Index | -0.61 |
Hydropathy Index | 0.54 |
Aliphatic Index | 117.14 |
Instability Index | 18.82 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 12490 M-1 cm-1 |
Absorbance 280nm | 181.01 |
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