Bacteriocin: Aureocin A53
Accession: BAC141
Producer Organism: Staphylococcus aureus [Gram-positive]
Taxonomy: BacteriaFirmicutes
Bacillales
Staphylococcus
Target organismsListeria monocytogenes - Micrococcus luteus (MIC=0.15 nM) - Staphylococcus simulans (MIC=100 nM) - Lactococcus lactis ATCC 19257 (MIC=0.25µM) - Brochothrix campestris ATCC 43754 (MIC=2µM) - Carnobacterium divergens LV13 (MIC=8µM) - Lactobacillus sakei UAL1218 (MIC=8µM) - Carnobacterium maltaromaticum UAL26 (MIC=128µM) - Enterococcus faecium BFE900 (MIC=16µM) - Staphylococcus aureus ATCC 29213 (MIC=128µM) -
NOTE: not active against Carnobacterium maltaromaticum UAL26 (pMG36c-cclBITCDA) - Enterococcus faecalis ATCC 7080 - Enterococcus faecalis 710C - Lactobacillus acidophilus M46 - Staphylococcus aureus ATCC 6538
It does not contain post-translationally modified amino acid residues such as lanthionine or â-methyllanthionine, as frequently found in modified bacteriocins (Lantibiotics).
MALDI-TOF analysis revealed a mass of 6012.5(±0.5) Da. Presence of an N-terminal formylmethionine residue.
Aureocin A53 showed extraordinary resistance to various proteases, in spite of the presence of suitable cleavage sites e.g. for trypsin.
UniProt Entry: Q8GPI4
PDB Entry2N8O resolved by NMR
PubMed=12054867;DOI=10.1016/S0022-2836(02)00368-6
Daili Jacqueline Aguilar Netz, Regula Pohl, Annette G. Beck-Sickinger, Thorsten Selmer, Antonio J. Pierik, Maria do Carmo de Freire Bastos and Hans-Georg Sahl.
"Biochemical Characterisation and Genetic Analysis of Aureocin A53, a New, Atypical Bacteriocin from Staphylococcus aureus", Journal of Molecular Biology 2002, 319 (3): 745-756.
PubMed=26771761;DOI=10.1021/acs.biochem.5b01306
Acedo J.Z., van Belkum M.J., Lohans C.T., Towle K.M., Miskolzie M., Vederas J.C.
"Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.", Biochemistry 2016, ():.
Gene id | Name | Description | Location |
BACGene615 | MobC-like protein mobilization-associated protein | 220..603 | |
BACGene616 | MobA-like protein mobilization-associated protein | 585..1592 | |
BACGene617 | MobB-like protein mobilization-associated protein | 1294..2109 | |
BACGene618 | replication protein Rep initiator of replication | 2808..3665 | |
BACGene619 | unknown | 3789..3944 | |
BACGene620 | SAP029-like protein similar to Staphylococcus aureus subsp. aureus N315 plasmid pN315 SAP029 protein | 4050..4235 | |
BACGene621 | YdbS-like protein similar to Bacillus subtilis YdbS protein | 4472..4969 | |
BACGene622 | YdbT-like protein similar to Bacillus subtilis YdbT protein | 4962..6389 | |
BACGene623 | aucA | aureocin A53 AucA | 6467..6622 |
BACGene624 | aucA | unknown | complement(6873..7163) |
BACGene625 | aucA | unknown orf11 | complement(7164..7448) |
BACGene626 | aucA | SA0191/BacG-like protein similar to Staphylococcus aureus subsp. aureus N315 SA0191 protein and toEnterococcus faecalis plasmid pPD1 BacG protein; contains one transmembranesegment | 7571..8566 |
BACGene627 | aucA | putative ABC transporter ATP-binding protein | 8563..9243 |
BACGene628 | aucA | SA0193/BacI-like protein similar to Staphylococcus aureus subsp. aureus N315 SA0193 protein and toEnterococcus faecalis plasmid pPD1 BacI protein; contains four transmembranesegments | 9240..10406 |
........10 ........20 ........30 ........40 ........50 ........60 | | | | | | MSWLNFLKYI AKYGKKAVSA AWKYKGKVLE WLNVGPTLEW VWQKLKKIAG L |
Feature | Position(s) | Length | Description |
CHAIN | 1↔51 | 51 | Bacteriocin aureocin A53. Feature identifier = PRO_0000356957.
|
Modified residue | 1 | N-formylmethionine. |
Formula | C291
H447
N69
O65
S1 |
Absent amino acids | CDHR |
Common amino acids | K |
Mass (Da) | 6012.5 |
Net charge | +8 |
Isoelectric point | 10.73 |
Basic residues | 10 |
Acidic residues | 2 |
Hydrophobic residues | 24 |
Polar residues | 12 |
Aliphatic residues | 13 |
Tiny residues | 11 |
Boman Index | -7.5 |
Hydropathy Index | -0.08 |
Aliphatic Index | 101.37 |
Instability Index | -3.89 (stable) |
Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
Extinction Coefficient | 31970 M-1 cm-1 |
Absorbance 280nm | 639.4 |
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