Bacteriocin: Aureocin A53

Default view

Bacteriocin: Aureocin A53

Accession: BAC141

Classification
Class: Unclassified
Genetics
Gene: aucA
Producer and target organisms

Producer Organism: Staphylococcus aureus [Gram-positive]

Taxonomy: BacteriaFirmicutesBacillalesStaphylococcus

Target organismsListeria monocytogenes - Micrococcus luteus (MIC=0.15 nM) - Staphylococcus simulans (MIC=100 nM) - Lactococcus lactis ATCC 19257 (MIC=0.25µM) - Brochothrix campestris ATCC 43754 (MIC=2µM) - Carnobacterium divergens LV13 (MIC=8µM) - Lactobacillus sakei UAL1218 (MIC=8µM) - Carnobacterium maltaromaticum UAL26 (MIC=128µM) - Enterococcus faecium BFE900 (MIC=16µM) - Staphylococcus aureus ATCC 29213 (MIC=128µM) -

NOTE: not active against Carnobacterium maltaromaticum UAL26 (pMG36c-cclBITCDA) - Enterococcus faecalis ATCC 7080 - Enterococcus faecalis 710C - Lactobacillus acidophilus M46 - Staphylococcus aureus ATCC 6538

Description
Aureocin A53, is a new tryptophan-rich anti-bacterial peptide that shares some characteristics with class IIa bacteriocins. It's noteworthy, the absence of a leader peptide from aureocin A53.

It does not contain post-translationally modified amino acid residues such as lanthionine or -methyllanthionine, as frequently found in modified bacteriocins (Lantibiotics).

MALDI-TOF analysis revealed a mass of 6012.5(0.5) Da. Presence of an N-terminal formylmethionine residue.

Aureocin A53 showed extraordinary resistance to various proteases, in spite of the presence of suitable cleavage sites e.g. for trypsin.
Uniprot and PDB links

UniProt Entry: Q8GPI4

PDB Entry2N8O resolved by NMR

STRUCTURE
PubMed=26771761;DOI=10.1021/acs.biochem.5b01306
Acedo J.Z., van Belkum M.J., Lohans C.T., Towle K.M., Miskolzie M., Vederas J.C.
"Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity.", Biochemistry 2016, ():.
Loading...
End Note
Reference Manager
BibTeX
Gene structure
genome of Aureocin A53

Gene idNameDescriptionLocation
BACGene615MobC-like protein mobilization-associated protein220..603
BACGene616MobA-like protein mobilization-associated protein585..1592
BACGene617MobB-like protein mobilization-associated protein1294..2109
BACGene618replication protein Rep initiator of replication2808..3665
BACGene619unknown3789..3944
BACGene620SAP029-like protein similar to Staphylococcus aureus subsp. aureus N315 plasmid pN315 SAP029 protein4050..4235
BACGene621YdbS-like protein similar to Bacillus subtilis YdbS protein4472..4969
BACGene622YdbT-like protein similar to Bacillus subtilis YdbT protein4962..6389
BACGene623aucAaureocin A53 AucA6467..6622
BACGene624aucAunknowncomplement(6873..7163)
BACGene625aucAunknown orf11complement(7164..7448)
BACGene626aucASA0191/BacG-like protein similar to Staphylococcus aureus subsp. aureus N315 SA0191 protein and toEnterococcus faecalis plasmid pPD1 BacG protein; contains one transmembranesegment7571..8566
BACGene627aucAputative ABC transporter ATP-binding protein8563..9243
BACGene628aucASA0193/BacI-like protein similar to Staphylococcus aureus subsp. aureus N315 SA0193 protein and toEnterococcus faecalis plasmid pPD1 BacI protein; contains four transmembranesegments9240..10406
aucAaucAaucAaucAaucAaucA
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 
          |          |          |          |          |          | 
 MSWLNFLKYI AKYGKKAVSA AWKYKGKVLE WLNVGPTLEW VWQKLKKIAG L

Wheel representation
wheel representation of Aureocin A53
3D Structure
Protein sequence annotations
Features of Aureocin A53

M
FeaturePosition(s)LengthDescription
CHAIN1↔5151
Bacteriocin aureocin A53.
Feature identifier = PRO_0000356957.
Modified residue1N-formylmethionine.
Composition
Hydrophobicity

Composition
Formula C291 H447 N69 O65 S1
Absent amino acids CDHR
Common amino acids K
Mass (Da) 6012.5
Net charge +8
Isoelectric point 10.73
Basic residues 10
Acidic residues 2
Hydrophobic residues 24
Polar residues 12
Aliphatic residues 13
Tiny residues 11
Boman Index -7.5
Hydropathy Index -0.08
Aliphatic Index 101.37
Instability Index -3.89 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 31970 M-1 cm-1
Absorbance 280nm 639.4

Users comments for Aureocin A53

No comments found

Login or register to submit a comment for Aureocin A53.