Bacteriocin: Propionicin SM1
Accession: BAC136
Classification
Class: Unclassified
Genetics
Gene: PpnA
Producer and target organisms
Producer Organism: Propionibacterium jensenii [Gram-positive]
Taxonomy: Bacteria
ActinobacteriaActinobacteridaeActinomycetalesPropionibacterineaePropionibacteriaceaePropionibacterium
Target organismsPropionibacterium jensenii DSM20274
Description
Heat-stable protein solated from Swiss raw milk.
Uniprot and PDB links
UniProt Entry: Q9L454
PDB EntryUnknown
NUCLEOTIDE SEQUENCE., STRAIN=DF1.
MEDLINE=20383893;PubMed=10930068;
Miescher S., Stierli M.P., Teuber M., Meile L.
"Propionicin SM1, a bacteriocin from Propionibacterium jensenii DF1: isolation and characterization of the protein and its gene.", Syst. Appl. Microbiol. 23:174-184(2000).
MEDLINE=20383893;PubMed=10930068;
Miescher S., Stierli M.P., Teuber M., Meile L.
"Propionicin SM1, a bacteriocin from Propionibacterium jensenii DF1: isolation and characterization of the protein and its gene.", Syst. Appl. Microbiol. 23:174-184(2000).
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Gene structure
| Gene id | Name | Description | Location |
| BACGene604 | hypothetical protein ORF8 | 155..388 | |
| BACGene605 | hypothetical protein ORF10 | 372..635 | |
| BACGene606 | hypothetical protein ORF9 | complement(700..1338) | |
| BACGene607 | putative theta replicase ORF1 | 1776..2684 | |
| BACGene608 | hypothetical protein ORF2 | 2684..3028 | |
| BACGene609 | hypothetical protein ORF3 | 3025..3552 | |
| BACGene610 | Propionicin SM1 PpnA | 4051..4674 | |
| BACGene611 | hypothetical protein ORF5 | 4727..5053 | |
| BACGene612 | putative invertase-like enzyme ORF6 | 5212..5757 | |
| BACGene613 | hypothetical protein ORF7 | complement(5966..6571) |
Protein sequence
| ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 | | | | | | | | MNKTHKMATL VIAAILAAGM TAPTAYADSP GNTRITASEQ SVLTQILGHK PTQTEYNRYV ETYGSVPTEA DINAYIEASE SEGSSSQTAA HDDSTSPGTS TEIYTQAAPA RFSMFFLSGT WITRSGVVSL SLKPRKGGIG NEGDERTWKT VYDKFHNAGQ WTRYKNNGVD ASMKKQYMCH FKYGMVKTPW NLEPHKKAAD VSPVKCN |
Composition
Hydrophobicity
Composition
| Formula | C995
H1544
N276
O314
S9 |
| Absent amino acids | |
| Common amino acids | TAS |
| Mass (Da) | 22706.84 |
| Net charge | +9 |
| Isoelectric point | 8.94 |
| Basic residues | 28 |
| Acidic residues | 19 |
| Hydrophobic residues | 58 |
| Polar residues | 78 |
| Aliphatic residues | 28 |
| Tiny residues | 55 |
| Boman Index | -364.62 |
| Hydropathy Index | -0.57 |
| Aliphatic Index | 57.58 |
| Instability Index | 43.75 (unstable) |
| Half Life |
Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour |
| Extinction Coefficient | 37025 M-1 cm-1 |
| Absorbance 280nm | 179.73 |
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