Bacteriocin: Colicin-M

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Bacteriocin: Colicin-M

Accession: BAC135

Classification
Class: Unclassified
Genetics
Gene: cma
Description
colicin M contains a pentapeptide (Glu-Thr-Leu-Thr-Val) in the domain required for uptake into cells.
A similar sequence was found in all colicins which are taken up by a TonB-dependent mechanism and in outer membrane receptor proteins which are constituents of TonB-dependent transport systems.
The structure of colicin M in the carboxy-terminal activity domain had no resemblance to the pore-forming colicins or colicins with endonuclease activity.
NUCLEOTIDE SEQUENCE, [GENOMIC DNA].
MEDLINE=87250310;PubMed=3036784;
RA Koeck J., Oelschlaeger T., Kamp R.M., Braun V.
"Primary structure of colicin M, an inhibitor of murein biosynthesis.", J. Bacteriol. 169:3358-3361(1987).
3D STRUCTURE
PubMed=21149455;DOI=10.1074/jbc.M110.165274
Helbig S., Patzer S.I., Schiene-Fischer C., Zeth K., Braun V.
"Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.", J.Biol.Chem. 286: 6280-6290 (2011).
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Gene structure
genome of Colicin-M

Gene idNameDescriptionLocation
BACGene603colicin M activity peptide60..875
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
          |          |          |          |          |          |          |          | 
 METLTVHAPS PSTNLPSYGN GAFSLSAPHV PGAGPLLVQV VYSFFQSPNM CLQALTQLED YIKKHGASNP LTLQIISTNI
 GYFCNADRNL VLHPGISVYD AYHFAKPAPS QYDYRSMNMK QMSGNVTTPI VALAHYLWGN GAERSVNIAN IGLKISPMKI
 NQIKDIIKSG VVGTFPVSTK FTHATGDYNV ITGAYLGNIT LKTEGTLTIS ANGSWTYNGV VRSYDDKYDF NASTHRGIIG
 ESLTRLGAMF SGKEY

3D Structure
Protein sequence annotations

FeaturePosition(s)LengthDescription
CHAIN1↔271271
Colicin-M.
Feature identifier = PRO_0000218689.
MOTIF2↔98TonB box.
TURN11↔133
HELIX18↔214
HELIX38↔458
HELIX49↔6517
STRAND67↔693
HELIX70↔9223
HELIX98↔1047
HELIX109↔1113
HELIX114↔1174
STRAND120↔1223
HELIX128↔13811
STRAND144↔1463
HELIX148↔1503
HELIX157↔1593
HELIX161↔1688
STRAND173↔18412
HELIX185↔1873
HELIX190↔1956
STRAND198↔20912
STRAND213↔23119
TURN232↔2343
HELIX237↔24913
STRAND255↔2584
STRAND263↔2708
Composition
Hydrophobicity

Composition
Formula C1324 H2059 N353 O392 S9
Absent amino acids
Common amino acids GSLIT
Mass (Da) 29505.15
Net charge +13
Isoelectric point 9.5
Basic residues 30
Acidic residues 17
Hydrophobic residues 89
Polar residues 104
Aliphatic residues 54
Tiny residues 65
Boman Index -276.94
Hydropathy Index -0.13
Aliphatic Index 86.01
Instability Index 38.09 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 33475 M-1 cm-1
Absorbance 280nm 123.98

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