Bacteriocin: Colicin-M

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Bacteriocin: Colicin-M

Accession: BAC135

Classification

Class: ~~Unclassified~~

Genetics

Gene: cma

Producer and target organisms

Producer Organism: Escherichia coli [Gram-negative]

Taxonomy: BacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Target organismsEnterobacteriaceae - Escherichia coli

Description

colicin M contains a pentapeptide (Glu-Thr-Leu-Thr-Val) in the domain required for uptake into cells.
A similar sequence was found in all colicins which are taken up by a TonB-dependent mechanism and in outer membrane receptor proteins which are constituents of TonB-dependent transport systems.
The structure of colicin M in the carboxy-terminal activity domain had no resemblance to the pore-forming colicins or colicins with endonuclease activity.

Uniprot and PDB links

UniProt Entry: P05820

PDB Entry2XMX resolved by X-RAY 2XTQ resolved by X-RAY 2XTR resolved by X-RAY 3DA3 resolved by X-RAY 3DA4 resolved by X-RAY

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, [GENOMIC DNA].
MEDLINE=87250310;PubMed=3036784;
RA Koeck J., Oelschlaeger T., Kamp R.M., Braun V.
"Primary structure of colicin M, an inhibitor of murein biosynthesis.", J. Bacteriol. 169:3358-3361(1987).

[Ref. 2] | View abstract | Export citation

3D STRUCTURE
PubMed=21149455;DOI=10.1074/jbc.M110.165274
Helbig S., Patzer S.I., Schiene-Fischer C., Zeth K., Braun V.
"Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.", J.Biol.Chem. 286: 6280-6290 (2011).

Gene structure

Gene id	Name	Description	Location
BACGene603		colicin M activity peptide	60..875

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80
| | | | | | | |
METLTVHAPS PSTNLPSYGN GAFSLSAPHV PGAGPLLVQV VYSFFQSPNM CLQALTQLED YIKKHGASNP LTLQIISTNI
GYFCNADRNL VLHPGISVYD AYHFAKPAPS QYDYRSMNMK QMSGNVTTPI VALAHYLWGN GAERSVNIAN IGLKISPMKI
NQIKDIIKSG VVGTFPVSTK FTHATGDYNV ITGAYLGNIT LKTEGTLTIS ANGSWTYNGV VRSYDDKYDF NASTHRGIIG
ESLTRLGAMF SGKEY

3D Structure

Protein sequence annotations

Feature	Position(s)	Length	Description
CHAIN	1↔271	271	Colicin-M. Feature identifier = PRO_0000218689.
MOTIF	2↔9	8	TonB box.
TURN	11↔13	3
HELIX	18↔21	4
HELIX	38↔45	8
HELIX	49↔65	17
STRAND	67↔69	3
HELIX	70↔92	23
HELIX	98↔104	7
HELIX	109↔111	3
HELIX	114↔117	4
STRAND	120↔122	3
HELIX	128↔138	11
STRAND	144↔146	3
HELIX	148↔150	3
HELIX	157↔159	3
HELIX	161↔168	8
STRAND	173↔184	12
HELIX	185↔187	3
HELIX	190↔195	6
STRAND	198↔209	12
STRAND	213↔231	19
TURN	232↔234	3
HELIX	237↔249	13
STRAND	255↔258	4
STRAND	263↔270	8

Composition

Hydrophobicity

Composition

Formula	C₁₃₂₄ H₂₀₅₉ N₃₅₃ O₃₉₂ S₉
Absent amino acids
Common amino acids	GSLIT
Mass (Da)	29505.15
Net charge	+13
Isoelectric point	9.5
Basic residues	30
Acidic residues	17
Hydrophobic residues	89
Polar residues	104
Aliphatic residues	54
Tiny residues	65
Boman Index	-276.94
Hydropathy Index	-0.13
Aliphatic Index	86.01
Instability Index	38.09 (stable)
Half Life	Mammalian : 30 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	33475 M^-1 cm^-1
Absorbance 280nm	123.98

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