Bacteriocin: Enterolysin A

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Bacteriocin: Enterolysin A

Accession: BAC133

Classification

Class: class III

Genetics

Gene: enlA

Producer and target organisms

Producer Organism: Enterococcus faecalis (Streptococcus faecalis) [Gram-positive]

Taxonomy: BacteriaFirmicutesLactobacillalesEnterococcaceaeEnterococcus

Target organismsLactobacillus sakei - Lactobacillus brevis - Lactobacillus curvatus - Lactococcus cremoris - Lactococcus lactis - Pediococcus pentosaceus - Pediococcus acidilactici - Enterococcus faecium - Enterococcus faecalis - Listeria innocua - Listeria ivanovii - Bacillus subtilis - Bacillus cereus - Staphylococcus carnosus - Propionibacterium jensenii -

NOTE:not active against Lactobacillus fermentum - Lactobacillus plantarum - Lactobacillus acidophilus - Leuconostoc lactis - Carnobacterium piscicola - Propionibacterium freudenreichii - Propionibacterium acidipropionici - Propionibacterium thoenii - Clostridium butyricum - Clostridium sporogenes - Clostridium bifermentans - Clostridium tyrobutyricum

Description

enterolysin A is a heat-labile protein with a broad inhibitory spectrum, which breaks down the cell walls of sensitive bacteria

Uniprot and PDB links

UniProt Entry: Q9F8B0

PDB Entry~~Unknown~~

[Ref. 1] | View abstract | Export citation

NUCLEOTIDE SEQUENCE, STRAIN=LMG 2333.
PubMed=12732574;DOI=10.1128/AEM.69.5.2975-2984.2003
Nilsen T., Nes I.F., Holo H.
"Enterolysin A, a cell wall-degrading bacteriocin from Enterococcus faecalis LMG 2333.", Appl. Environ. Microbiol. 69:2975-2984(2003).

Gene structure

Gene id	Name	Description	Location
BACGene594	scpE	staphopain peptidase virulence factor	271..1767
BACGene595	scpE	hypothetical protein ORF1	2056..2238
BACGene596	scpE	hypothetical protein ORF2	2533..2790
BACGene597	entL	enterolysin A peptidase M23 family	3041..4072
BACGene598	entL	hypothetical protein conserved domain protein ORF3	4243..4773

Protein sequence

........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80
| | | | | | | |
ASNEWSWPLG KPYAGRYEEG QQFGNTAFNR GGTYFHDGFD FGSAIYGNGS VYAVHDGKIL YAGWDPVGGG SLGAFIVLQA
GNTNVIYQEF SRNVGDIKVS TGQTVKKGQL IGKFTSSHLH LGMTKKEWRS AHSSWNKDDG TWFNPIPILQ GGSTPTPPNP
GPKNFTTNVR YGLRVLGGSW LPEVTNFNNT NDGFAGYPNR QHDMLYIKVD KGQMKYRVHT AQSGWLPWVS KGDKSDTVNG
AAGMPGQAID GVQLNYITPK GEKLSQAYYR SQTTKRSGWL KVSADNGSIP GLDSYAGIFG EPLDRLQIGI SQSNPF

Composition

Hydrophobicity

Composition

Formula	C₁₅₅₂ H₂₃₂₉ N₄₂₉ O₄₆₁ S₄
Absent amino acids	C
Common amino acids	G
Mass (Da)	34524.69
Net charge	+13
Isoelectric point	9.64
Basic residues	37
Acidic residues	24
Hydrophobic residues	92
Polar residues	125
Aliphatic residues	51
Tiny residues	87
Boman Index	-476.75
Hydropathy Index	-0.57
Aliphatic Index	62.94
Instability Index	23.5 (stable)
Half Life	Mammalian : 4.4 hour Yeast : >20 hour E. coli : >10 hour
Extinction Coefficient	77350 M^-1 cm^-1
Absorbance 280nm	245.56

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