Bacteriocin: Colicin-10

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Bacteriocin: Colicin-10

Accession: BAC131

Class: Unclassified
Gene: cta
The ion-channel forming colicins A, B, E1, Ia, Ib and N all kill bacterial cells selectively by co-opting bacterial active-transport pathways and forming voltage-gated ion conducting channels across the plasma membrane of the target bacterium. The crystal structure of colicin Ia reveals a molecule 210 A long with three distinct functional domains arranged along a backbone of two extraordinarily long alpha-helices. A central domain at the bend of the hairpin-like structure mediates specific recognition and binding to an outer-membrane receptor. A second domain mediates translocation across the outer membrane via the TonB transport pathway; the TonB-box recognition element of colicin Ia is on one side of three 80 A-long helices arranged as a helical sheet. A third domain is made up of 10 alpha-helices which form a voltage-activated and voltage-gated ion conducting channel across the plasma membrane of the target cell. The two 160 A-long alpha-helices that link the receptor-binding domain to the other domains enable the colicin Ia molecule to span the periplasmic space and contact both the outer and plasma membranes simultaneously during function.
Uniprot and PDB links

UniProt Entry: Q47125

PDB EntryUnknown

End Note
Reference Manager
Gene structure
genome of Colicin-10

Gene idNameDescriptionLocation
BACGene590ctacolicin 10446..1918
BACGene591cticolicin 10 immunity proteincomplement(1915..2205)
BACGene592ctlcolicin 10 lysis protein2230..2361
Protein sequence
 ........10 ........20 ........30 ........40 ........50 ........60 ........70 ........80 
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Protein sequence annotations

Feature identifier = PRO_0000218673.
TRANSMEM447↔46721Helical; (Potential).

Formula C2333 H3753 N657 O759 S6
Absent amino acids C
Common amino acids A
Mass (Da) 53369.26
Net charge -6
Isoelectric point 5.1
Basic residues 74
Acidic residues 80
Hydrophobic residues 187
Polar residues 115
Aliphatic residues 36
Tiny residues 86
Boman Index -971.84
Hydropathy Index -0.49
Aliphatic Index 81.2
Instability Index 20.98 (stable)
Half Life Mammalian : 30 hour
Yeast : >20 hour
E. coli : >10 hour
Extinction Coefficient 47900 M-1 cm-1
Absorbance 280nm 97.96

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